Tetrahydroaminoacridine improves passive avoidance retention defects induced by aging and medial septal lesion but not by fimbria-fornix lesion.

The present study examines whether tetrahydroaminoacridine (THA) can improve the deterioration in passive avoidance (PA) retention performance induced by medial septal (MS) and fimbria-fornix (FF) lesions in young rats or by aging. Retention of young MS-lesioned rats was improved by pretraining injection of THA at 3 mg/kg, but not by THA at 1 mg/kg or by either of the posttraining doses of THA (1 and 3 mg/kg). Pretraining injections of THA at 1 or 3 mg/kg had no effect on the PA retention performance of FF-lesioned rats. Age-induced PA failure was alleviated by pretraining administration of THA at 1 and 3 mg/kg. Posttraining injections of THA (1 or 3 mg/kg) had no effect on PA retention performance of aged rats. These results demonstrate that 1) THA may improve hippocampal cholinergic denervation-induced functional deficits and 2) some of the age-related PA deficits may be due to a cholinergic deficit and can be reversed with THA.     

Spatial Learning and Noradrenaline Content in the Brain and Periphery of Young and Aged Rats

The present experiment studied whether a dysfunction of the noradrenergic neurons is related to spatial learning impairment by investigating the levels of noradrenaline in the brain and periphery as well as the acquisition of water maze task in saline-pretreated young rats, in noradrenergic neurotoxin (DSP-4)-pretreated young rats and in saline-pretreated aged rats. Aged rats, which had an increased escape latency onto the hidden platform, revealed a decreased noradrenaline content in the heart, but not in the hippocampus, striatum, or hypothalamus, whereas DSP-4-pretreated rats had decreased noradrenaline content in the brain; the acquisition of water maze task was not impaired. These results suggest that the peripheral noradrenergic system can show age-related changes different from those in the central noradrenergic system, and they failed to provide support for the hypothesis that decreased activity of the central noradrenergic nerves is related to impairment in the acquisition of the water maze task.     

Age-dependent stimulation by atrium explants or nerve growth factor of nerve fibre outgrowth from cocultured embryonic rat sympathetic ganglia

Whole sympathetic superior cervical ganglia of 14- and 15-day-old rat embryos were cultured in a collagen gel medium for 24 h with or without explants of heart atrium from the same animals or from newborn rats. The extent of nerve fibre outgrowth was estimated by counting the number of nerve fibres crossing each arc of a sector drawn in the microscope ocular. Only few nerve fibres extended from the ganglia of the 14-day-old embryos cultured in the pure control medium. Addition of exogenous nerve growth factor did not stimulate the poor growth. While the growth of nerve fibres in the presence of atrium explants of newborn rats did not significantly differ from that in cultures without any target tissue, the presence of atrium explants of the same, 14-day-old, embryo resulted in clearly enhanced fibre outgrowth from the ganglia, which was inhibited by antiserum to the nerve growth factor. The ganglia of 15-day-old embryos cultured without target tissue in the control medium produced only sparse fibre outgrowth which was excessively increased by exogenous nerve growth factor. Coculture with atrium explants of 15-day-old embryos or newborn rats produced an increased overall nerve fibre growth, as well as predominance of those fibres growing toward the target tissue. The growth-promoting effect of the atrium of 15-day-old embryos was only partially prevented by antiserum to the nerve growth factor, while that elicited by newborn rat atrium was totally inhibited.(ABSTRACT TRUNCATED AT 250 WORDS)