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Laval-Gilly P Falla J Klestadt D Henryon M 《Journal of pharmacological and toxicological methods》2000,44(3):483-488
A novel technique is described that measures the degree of toxicity of short-term exposure to gaseous pollutants or other chemical compounds on cultured cells, in 30 min. This technique, based on the study of the mobility properties of activated macrophages, consists of an image analysis procedure incorporating a specific exposure chamber (EC). The EC, which is developed from commercial culture flasks (50 ml, 25 cm(2) of culture surface), was first used to maintain cells in culture conditions, overnight, prior to the assay. In order to measure toxicity, it was then connected to the gaseous pollutant or chemical source. After exposing the culture medium and cells to the gas stream for 10 min, fMLP, a chemotactic factor, was added and the mobility of the macrophages measured by superimposing sequential analogue images captured by a CCD camera that were digitised and analysed using a software developed for this purpose. For example, the effect of ozone on macrophage-like cell (THP-1) was investigated. After exposure to 0.1 and 0.5 ppm, cells lost, respectively 79% and 90% of their mobility, compared to the control sample. 相似文献
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Ziemke P. Fraenckel Panse Weimann Jendralski Vorschütz Wachholz B. Mueller Giese Schwarz Hans Strauss Hecht Wietrich Urechia Kurt Mendel F. Stern Ostertag Tietze Kehrer Einar Sjövall G. Michelsson v. Neureiter Klestadt Ruge Kappus Zillmer Reisner Otte H. Merkel 《International journal of legal medicine》1933,21(2-3):85-100
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Mller-Hess Ziemke Gutzeit R. Riebeling Stern F. Klieneberger Bratz Gierlich Mendel Kurt Flatau G. Wiethold Kockel Heinz Binswanger H. Klestadt Oblath Koch Lurz L. Buhtz Brieger Schellenberg Duschl L. Strassmann Gg. Reich Liguori-Hohenauer Baruch v. Neureiter Schwarz O. A. Zavka Ruge Ullmann K. 《International journal of legal medicine》1932,18(6):271-283
International Journal of Legal Medicine - 相似文献
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Reich Wortmann Ö. Fischer Walter Lehmann Ritter Schmincke Marx Mendel F. Stern Hiller Breitenecker Weimann Klestadt Otto Dyes Breitländer Goebel Burghard Wachholz Eisner Scheuer Weimann H. Brach Heinz Lossen Colmers Kempf Hecht Josef Wilder Adolf Friedemann Else Petri Schrader Hans Goldmann Foerster H. Merkel Kurt Mendel 《International journal of legal medicine》1932,19(5):296-307
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Nippe Klestadt Henneberg Jendralski Hans Kloiber Heidemann Foerster Trendtel Schrader Panse Schönberg G. Strassmann Hans Baumm Esser Endre Makai Arno Warstadt Hallervorden Steck Walcher Schlomka Franz Baniecki Spiecker Ernst A. Mueller P. Fraenckel Schrader Giese S. Fuhs Autoreferat Mayser Lamers Kalmus S. FuΒ Révész Panse Estler H. Scholz Schwarzacher Wierig H. E. Lorenz Lochte 《International journal of legal medicine》1935,24(2-3):168-189
Ohne Zusammenfassung 相似文献
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Gollwitzer Merkel Ziemke E. Küppers Arthur Schüller Taterka A. Hoche Küppers E. Braun Bálint Kurt Mendel H. -V. Wagner E. Loewy-Hattendorf E. Heymann Fr. Wohlwill Klestadt V. Tanturri F. Jendralski Merz Oblath Buhtz Trendtel Weimann J. Prissmann Glogauer Eisenlauer Helmut Schmidt Werner Block Heller 《International journal of legal medicine》1930,14(2):57-66
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Schönberg Kurt Mendel Miescher Koch Keβler Klestadt F. Stern Arthur Stern Pfister Reichardt M. Seyfarth Karl M. Walthard Zingerle Krambach A. Freund Ziemke Plenz Schellenberg F. Wiethold S. Isaac H. Steinitz L. Duschl Nagel Baruch Panse Böhmer Gustav Rosenburg A. Meyer 《International journal of legal medicine》1932,19(2):119-128
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Walcher Schnberg Timm Giese Ziemke Mendel Kurt Gewel Taterka Hans Hiller Pfister Stern F. Urechia C. I. Killmann Pflimlin Panse Klestadt Lochte Eisner Foerster Ruge Hellner Strassmann G. Scholz H. Vorschtz Siegert F. Lindemann 《International journal of legal medicine》1933,22(1):159-170
Ohne Zusammenfassung 相似文献
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