全文获取类型
收费全文 | 1807篇 |
免费 | 106篇 |
国内免费 | 7篇 |
专业分类
耳鼻咽喉 | 30篇 |
儿科学 | 38篇 |
妇产科学 | 33篇 |
基础医学 | 217篇 |
口腔科学 | 43篇 |
临床医学 | 147篇 |
内科学 | 523篇 |
皮肤病学 | 27篇 |
神经病学 | 55篇 |
特种医学 | 91篇 |
外科学 | 313篇 |
综合类 | 46篇 |
一般理论 | 2篇 |
预防医学 | 84篇 |
眼科学 | 28篇 |
药学 | 158篇 |
中国医学 | 12篇 |
肿瘤学 | 73篇 |
出版年
2024年 | 5篇 |
2023年 | 24篇 |
2022年 | 86篇 |
2021年 | 92篇 |
2020年 | 42篇 |
2019年 | 66篇 |
2018年 | 83篇 |
2017年 | 52篇 |
2016年 | 59篇 |
2015年 | 56篇 |
2014年 | 84篇 |
2013年 | 65篇 |
2012年 | 168篇 |
2011年 | 176篇 |
2010年 | 105篇 |
2009年 | 85篇 |
2008年 | 87篇 |
2007年 | 96篇 |
2006年 | 90篇 |
2005年 | 90篇 |
2004年 | 87篇 |
2003年 | 62篇 |
2002年 | 44篇 |
2001年 | 16篇 |
2000年 | 23篇 |
1999年 | 18篇 |
1998年 | 8篇 |
1997年 | 7篇 |
1996年 | 7篇 |
1995年 | 5篇 |
1994年 | 4篇 |
1993年 | 5篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1968年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有1920条查询结果,搜索用时 0 毫秒
1.
G?khan Tun?bilek Serdar Nasir Omer Ozkan Aycan Kayik?io?lu Emin Mavili 《Nordisk plastikkirurgisk forening [and] Nordisk klubb for handkirurgi》2004,38(2):94-99
Defects in the sacrococcygeal and ischial soft tissues can be treated with gluteus maximus and posterior thigh V-Y advancement flaps. However, late complications include recurrence and dehiscence of the suture line. Increasing the amount of the soft tissues over the bony prominences and multilayered closure may have an advantage for long-term durability. We modified the V-Y advancement technique by de-epithelialising the medial parts of the flap and burying them under the opposing edge of the wound or the flap. Sixteen patients with various defects of the sacrococcygeal and ischial soft tissues were operated on using this technique. All the flaps healed well with no partial or complete loss of the flap. Three patients developed complications. The main advantage of our technique is the use of healthy tissues to obliterate the dead spaces under the edges of the wound or the opposing flap. In this way, not only the defect in the skin but the defect in the subcutaneous tissue, with its iceberg tip at the surface, is treated effectively. To have an additional layer of tissue between the bone and the superficial tissues provides an extra cushion of soft tissue and avoids putting the suture line directly over the bony prominences. We used this modification safely for both unilateral and bilateral flaps. It could also be used successfully in other parts of the body. 相似文献
2.
3.
Nasir J 《Clinical genetics》2001,60(5):332-333
4.
5.
Random minicircle DNA molecules were released from isolated kinetoplast network DNA of Trypanosoma congolense by BamHI digestion and cloned into plasmid pUC19. The sequences of two cloned minicircles (958 bp and 964 bp) were determined. Both minicircles contain the 13 bp sequence, 5'-GGGGTTGGTGTAA-3', thought to be the replication origin of minicircles in other trypanosomatids. The two minicircles have extensive homology in the 120 bp preceeding, and the 20 bp following, this 13-mer but only scattered homology elsewhere. Both possess tandem repeats downstream of the 13-mer. Comparison of these minicircles with minicircle sequences from other trypanosomatids reveals that they have the same general sequence organization as the others although only the 13-mer and its flanking regions are homologous. 相似文献
6.
Fascin,an actin-bundling protein,modulates colonic epithelial cell invasiveness and differentiation in vitro 总被引:18,自引:0,他引:18 下载免费PDF全文
Jawhari AU Buda A Jenkins M Shehzad K Sarraf C Noda M Farthing MJ Pignatelli M Adams JC 《The American journal of pathology》2003,162(1):69-80
In epithelial tissue, cell-matrix and cell-cell adhesive interactions have important roles in the normal organization and stabilization of the cell layer. The malignant conversion of epithelial cells involves alterations in the expression and function of these adhesion systems that enable a switch to a migratory phenotype in tumor invasion and metastasis. Fascin is an actin-crosslinking protein that is found in the core actin bundles of cell-surface spikes and projections that are implicated in cell motility. We demonstrate that fascin is not detectable in normal colonic epithelium, but is dramatically up-regulated in colorectal adenocarcinoma. To test the hypothesis that fascin could participate in tumor invasive behavior, we developed a cell culture model to examine the effect of fascin expression on the adhesive interactions, invasiveness, and differentiation of colonic epithelial cells. We report marked effects on the organization of cell-surface protrusions, actin cytoskeleton, and focal adhesions in the absence of alterations in the protein levels of the major components of these structures. These effects correlate with alterations in cell movements on two-dimensional matrix, and increased invasiveness in three-dimensional matrix. The cells also show increased proliferation and decreased capacity for normal glandular differentiation in collagen gels. We propose that up-regulation of fascin, by promoting the formation of protrusive, actin-based, cell-motility structures, could be a significant component in the acquisition of invasive phenotype in colonic carcinoma. 相似文献
7.
The increasing prevalence of penicillin-resistant Streptococuus pneumoniae urges for fast and accurate susceptibility testing methods. This study evaluated the comparability of three commonly used techniques; disk diffusion, E-test and agar dilution, to detect penicillin susceptibility in clinical isolates of S. pneumoniae. Fifty pneumococcal isolates, obtained from patients at the University of Malaya Medical Centre, were selected to include both penicillin-susceptible strains and those that had decreased susceptibility (resistant and intermediate) to penicillin. The minimum inhibitory concentration (MIC) values of penicillin to serve as the reference was determined by the agar dilution method in which, based on the MIC breakpoints recommended by the National Committee for Clinical Laboratory Standards (NCCLS), 27 strains had decreased susceptibility to penicillin with 17 strains resistant and 10 intermediate. Comparing to the agar dilution method, oxacillin disk diffusion test detected all strains with decreased penicillin susceptibility as such while E-test showed a close agreement of susceptibility (92%) of the isolates to penicillin. This confirmed that oxacillin is a good screening test for S. pneumoniae isolates with decreased susceptibility to penicillin while E-test is very reliable for rapid and accurate detection of penicillin susceptibility. 相似文献
8.
9.
Abdullahi Idris Nasir Lozano Carmen Juárez-Fernández Guillermo Höfle Ursula Simón Carmen Rueda Silvia Martínez Angela Álvarez-Martínez Sandra Eguizábal Paula Martínez-Cámara Beatriz Zarazaga Myriam Torres Carmen 《European journal of clinical microbiology & infectious diseases》2023,42(5):569-581
European Journal of Clinical Microbiology & Infectious Diseases - This study determined the carriage rates and antimicrobial resistance (AMR) genes of enterococci from... 相似文献
10.
Ruck NF Hafeez M Manzur S Nasir F Rivers K Prozesky D 《Journal of health & population in developing countries》1999,2(1):78-87
Pakistan's public sector is organized in a federal system with many management and planning functions devolved to the 4 provincial governments. Provincial health secretariats lead on most policy and planning decisions for health services. The provinces employ health personnel, although the national Public Service Commission controls some key aspects of human resources management. Reporting the findings of a training needs assessment (TNA) for health personnel in the provincial health services of Punjab, the authors show how TNA can be used systematically to improve the quality of health professional training. They also discuss the extent to which better training could contribute to improved health management capacity in Pakistan, and describe the context and problems of Pakistan's health services, focusing upon management capacity, and the methods and results of a training needs assessment conducted to address the problems. A final section covers the usefulness of the TNA method in Pakistan and its applicability to other countries. Moreover, the implications of decentralization and the problems of preparing training plans in the absence of decentralized structures are discussed. 相似文献