Chronische Ulzerationen einer Gesichtshälfte

Ohne Zusammenfassung     

Physiologische Reaktionen nach der Blutentnahme

Zusammenfassung Zur Prüfung einer von den Amerikanernvan Slyke undPhillips angegebenen Schnellmethode zur Messung des menschlichens Bluteiweißes wurden 50 Blutspender untersucht und ihre physiologischen Reaktionen nach der Blutentnahme geprüft.Es konnte bestätigt werden, daß beim Blutverlust der Verringerung der kreisenden Blutmenge die entscheidende Bedeutung zukommt. Der Organismus ist bestrebt, die Leere im Kreislauf durch Ausschüttung hämoglobinreicherer Erythrocyten aus den Depotorganen, wie Leber, Milz und subpapillärem Plexus aufzufüllen.Durch Einströmen von Gewebswasser in die Blutbahn kehrt das Kreislaufvolumen zur ursprünglichen Norm zurück, die Erythrocyten verschwinden wieder in ihre Lagerstätten.Zur Erhaltung des kolloidosmotischen Druckes, d. h. zur Retention der eiweißärmeren Gewebsflüssigkeit, werden hydrationsfähige Proteine aus der Leber vornehmlich mobilisiert.Die Wiederauffüllung des Kreislaufs ist in erster Linie eine physikalische Notwendigkeit, zu deren Stabilisierung Gewebs- wie Plasmaproteine beitragen. Das weitere Absinken der Erythrocyten über den 2. Tag hinaus wird mit ihrem hohen Eiweißmolekül erklärt, das in den ersten Tagen nach der Blutentnahme als Plasmaprotein Verwendung findet.Die Tatsache, daß die hier gefundenen Ergebnisse mit bisherigen übereinstimmen, verbunden mit dem Vorteil der sicheren und schnellen Handhabung, lassen die Methode vonvan Slyke undPhillips für die Klinik brauchbar erscheinen.Mit 4 Textabbildungen.     

Release of soluble transferrin receptor from the surface of human leukemic HL60 cells

Information regarding transferrin (Tf) receptor degradation is largely incomplete. HL60 cells were shown to release to their growth medium a Tf-binding protein which could be immunoprecipitated by anti-Tf receptor monoclonal antibodies (MoAbs) B3/25 and OKT9. Soluble Tf receptor was detected in the medium within one hour of replating of cells, and its release was inhibited at 4 degrees C. The affinity of Tf for the soluble receptor released by cells (kd = 2.3 x 10(-10) mol/L) was slightly lower than its affinity for the detergent-solubilized cellular receptor (kd = 1.2 x 10(-10) mol/L). 125I-Tf internalized and released by cells subsequently bound to Tf receptor released by the same cells, and soluble Tf receptor in the conditioned medium (CM) inhibited 125I-Tf binding to intact cells. The soluble Tf receptor isolated from the CM was smaller (78,000 daltons) than the cell surface receptor (94,000 daltons) when analyzed by gel electrophoresis under reducing conditions. Isolated cell membranes readily released soluble receptor; however, this release could be blocked by protease inhibitors. The soluble Tf receptor may represent the extracytoplasmic domain of the cellular Tf receptor released from the surface of HL60 cells through proteolytic cleavage by a membrane-based protease.     

Pneumothorax and other lung diseases: effect of altered resolution and edge enhancement on diagnosis with digitized radiographs

Prior studies have shown that pneumothorax is one of the more difficult entities to diagnose with digitized radiography. This study was designed to test whether increasing resolution from 1.25 to 2.5 line pairs per millimeter (lp/mm) and image processing (edge enhancement from unsharp masking) would increase accuracy and confidence in the diagnosis of pneumothorax, as well as normal cases and other forms of lung disease. Conventional radiographs were digitized with use of a laser reader and then reformatted as film hard copy. Eleven observers read 35 cases reformatted in three different ways (1.25 lp/mm, 2.5 lp/mm, 1.25 lp/mm unsharp mask). The images with finer resolution (2.5 lp/mm) and unsharp mask images were superior to those with coarser resolution (1.25 lp/mm) for the diagnosis of pneumothorax. There was no difference in diagnostic accuracy for normal patients. For abnormalities other than pneumothorax, the unsharp mask images were significantly worse. Confidence in the diagnosis of pneumothorax and other abnormalities was highest with the finest resolution (2.5 lp/mm).