楮实对小鼠学习和记忆的促进作用

本试验采用食饵迷宫法和一次训练被动回避性条件反应——跳台法,观察了楮实对小鼠学习和记忆的促进作用。结果表明,楮实对正常小鼠的空间辨别学习、记忆获得有促进作用;可拮抗东莨菪碱造成的记忆获得障碍;改善氯霉素和亚硝酸钠造成的记忆巩固不良;改善30％乙醇引起的记忆再缺损,并对亚硝酸钠中毒缺氧有明显的改善作用。     

葛根素对LPS诱导急性脑损伤小鼠的保护作用

目的:研究葛根素(Pur)对脂多糖(LPS)诱导的小鼠急性感染性脑损伤的保护作用及机制。方法:36只清洁级雄性ICR小鼠随机分为正常对照组(NS)、LPS感染组(LPS)和葛根素组(Pur+LPS)。LPS腹腔注射建立急性脑损伤模型,立即腹腔注射给予葛根素注射液治疗,6和24h后旷场。暗场实验检测各组小鼠的自发活动,WesternBlot检测脑组织白细胞分化抗原14(CD14)和环氧化酶2(C0X-2)蛋白表达量,Nisl染色观察神经元存活情况。结果:与NS组相比,LPS组6和24 h旷场实验自主活动距离和穿格次数显著减少,海马神经元的存活数目显著减少,脑组织CD14和C0X-2蛋白表达量显著升高(P<0.05);与LPS组比较,Pur+LPS组6和24 h时海马神经元的存活率明显提高,且CD14和C0X-2蛋白表达量明显下降(P<0.05)。结论:葛根素注射液对LPS所致小鼠急性感染性脑损伤具有一定保护作用,其作用机制可能与抑制脑组织CD14和C0X-2表达有关。     

The forensic landscape and the population genetic analyses of Hainan Li based on massively parallel sequencing DNA profiling

International Journal of Legal Medicine - Due to the formation of the Qiongzhou Strait by climate change and marine transition, Hainan island was isolated from the mainland southern China during...     

The Effect of Glycated Hemoglobin and Albumin-Corrected Glycated Serum Protein on Mortality in Diabetic Patients Receiving Continuous Peritoneal Dialysis

♦ Objective:

To explore the effect of glycated hemoglobin (HbA1c) and albumin-corrected glycated serum proteins (Alb-GSP) on the mortality of diabetic patients receiving continuous peritoneal dialysis (PD).

♦ Methods:

In this single-center retrospective cohort study, incident diabetic PD patients from January 1, 2006, to December 31, 2010, were recruited, and followed up until December 31, 2011. The effect of HbA1c and Alb-GSP on mortality was evaluated by Cox proportional hazards models.

♦ Results:

A total of 200 patients (60% male, mean age 60.3 ± 10.6 years) with a mean follow-up of 29.0 months (range: 4.3 – 71.5 months) were recruited. Sixty-four patients died during the follow-up period, of whom 21 died of cardiovascular disease (CVD). Mean values for HbA1c, GSP and Alb-GSP were 6.7% (range: 4.1 – 12.5%), 202 μmol/L (range: 69 – 459 μmol/L), and 5.78 μmol/g (range: 2.16 – 14.98 μmol/g), respectively. The concentrations of GSP and Alb-GSP were closely correlated with HbA1c (r = 0.41, p < 0.001 and r = 0.45, p < 0.001, respectively). In multivariate Cox proportional hazards models, patients with HbA1c ≥ 8% were associated with increased risk of all-cause mortality (hazard ratio [HR] = 2.29, 95% confidence interval [CI]: 1.06 – 4.96, p = 0.04), but no increased mortality in patients with 6.0% ≤ HbA1c ≤ 7.9%. Patients with Alb-GSP ≤ 4.50 μmol/g had increased all-cause and non-cardiovascular mortality (HR = 2.42, 95% CI: 1.13 – 5.19, p = 0.02; and HR = 2.98, 95% CI: 1.05 – 8.48, p = 0.04 respectively).

♦ Conclusions:

Increased HbA1c and decreased Alb-GSP may be associated with poorer survival in diabetic PD patients, with a non-significant trend observed for poorer survival with the highest level of Alb-GSP.     

清开灵注射液联合地西泮对失眠症患者睡眠质量及单胺类神经递质的影响

目的：观察清开灵注射液联合地西泮对失眠症患者睡眠质量及单胺类神经递质的影响。方法：选取158 例失眠症患者,按随机数字表法分为对照组及试验组,各79 例。对照组用地西泮治疗,试验组用地西泮联合清开灵注射液治疗,均治疗1 个月。观察2 组临床疗效、不良反应及治疗前后匹兹堡睡眠质量量表（PSQI） 评分、简易智能精神状态检查量表（MMSE） 评分、多导睡眠图参数（睡眠潜伏期、睡眠总时间、睡眠效率、醒觉时间）、5-羟吲哚乙酸（5-HIAA）、γ-氨基丁酸（GABA）、多巴胺（DA）、T 淋巴细胞亚群（CD4+/CD8+、CD4+、CD3+、CD8+） 水平。结果：试验组治疗总有效率为88.61%,高于对照组的75.95%（P＜0.05）。与同组治疗前比较,治疗后2 组PSQI 评分及血清CD8+水平均降低（P＜0.05）,睡眠潜伏期、醒觉时间均缩短（P＜0.05）,睡眠总时间均延长（P＜0.05）,睡眠效率、MMSE 评分及血清5-HIAA、GABA、DA、CD4+、CD3+、CD4+/CD8+水平均升高（P＜0.05）;与对照组治疗后比较,试验组治疗后PSQI 评分及血清CD8+水平均较低（P＜0.05）,睡眠潜伏期、醒觉时间均较短（P＜0.05）,睡眠总时间较长（P＜0.05）,睡眠效率、MMSE 评分及血清5-HIAA、GABA、DA、CD4+、CD3+、CD4+/CD8+水平均较高（P＜0.05）。2 组不良反应发生率比较,差异无统计学意义（P＞0.05）。结论：清开灵注射液联合地西泮治疗失眠症临床疗效显著,可显著调节单胺类神经递质及T 淋巴细胞亚群水平,进而有效改善睡眠质量,延长睡眠时间,促使患者认知功能提升,且安全性良好。     

产科新生儿护理中应用Orem自理模式的临床观察

目的：对Orem 自理模式应用在产科新生儿护理当中的临床效果进行分析与探讨。方法选取我院2012年2月-2014年2月间收治的198例剖宫产分娩的产妇的临床资料进行回顾性分析,所有产妇随机分为对照组与观察两组,对照组100例给予常规临床护理干预,观察组98例在常规临床护理的基础上行Orem自理模式使之实现自我护理。一段护理时间后,对比两组产妇相关护理知识熟悉程度,如母乳喂养知识、对新生儿护理措施的熟悉程度。结果研究结果显示,观察组与对照进行比较,对照组的产妇对母乳喂养知识的掌握及对新生儿护理措施的掌握程度明显低于观察组,而观察组的产妇能够较熟练的掌握母乳喂养技巧及对新生儿护理的相关知识,对比两组的差异具有统计学意义（P＜0.05）。结论对Orem 自理模式应用在产科新生儿护理当中的临床效果显著,能够非常好的挖掘出产妇的独立护理能力,最大程度体现出了护士的临床价值,具有较好的护理效果,同时也融洽了护士与产妇之间的关系,提高了临床的医疗效果,值得临床大力推广。     

TYMS-TM4SF4 axis promotes the progression of colorectal cancer by EMT and upregulating stem cell marker

Background: The expression of thymidylate synthase (TYMS) is significantly up-regulated in various cancers and associated with the poor prognosis of patients. However, the role of TYMS in the progression of colorectal cancer (CRC) is unclear. Methods: Cell function assay, biology information analysis, and RNA sequencing were used to investigate the role of TYMS in the progression of CRC and underlining molecular mechanism. SPSS22.0 statistical software and GraphPad Prism 5 (Graphpad software) were used for statistical analysis. Results: Our results showed that TYMS expression was higher in CRC tissues than that in non-tumor colorectal mucosa tissues. TYMS knockdown inhibited the proliferation, migration and invasion of HCT116 and HT29 cells, and the spheroid formation of HCT116 cells. The underling mechanism demonstrated that TYMS promoted the progression of CRC by regulating EMT-related proteins including E-cadherin, Vimentin, MMP-9 and stem cell biomarkers including CD133 and CD44. Furthermore, DEG sequencing showed that TYMS knockdown enriched the pathways of metastasis and metabolism by GO and KEGG analysis. We identified TM4SF4 was the downstream target of TYMS in CRC cells. TM4SF4 overexpression increased migration and invasion of CRC cells by regulating EMT and CD133 expression. Conclusions: Our findings suggest that TYMS-TM4SF4 axis may promote the progression of CRC by EMT and upregulating stem cell markers.     

血管紧张素转化酶2在冠状病毒感染所致肺损伤及其他肺部疾病中研究进展

血管紧张素转化酶2(ACE2)为拮抗血管紧张素转化酶-血管紧张素(ACE-AngⅡ)的关键调控分子.本文总结ACE2通过与新型冠状病毒(SARS-CoV-2)以及非典型性肺炎(SARS)病毒所携带的S蛋白结合,介导上述病毒进入宿主细胞进行复制并发挥细胞毒性作用,并探讨使用二甲双胍以及血管紧张素转化酶抑制剂/血管紧张素受...