全文获取类型
收费全文 | 604篇 |
免费 | 23篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 14篇 |
儿科学 | 5篇 |
妇产科学 | 5篇 |
基础医学 | 42篇 |
口腔科学 | 26篇 |
临床医学 | 46篇 |
内科学 | 109篇 |
皮肤病学 | 3篇 |
神经病学 | 44篇 |
特种医学 | 159篇 |
外科学 | 57篇 |
综合类 | 13篇 |
预防医学 | 32篇 |
眼科学 | 5篇 |
药学 | 15篇 |
肿瘤学 | 53篇 |
出版年
2023年 | 4篇 |
2022年 | 9篇 |
2021年 | 22篇 |
2020年 | 13篇 |
2019年 | 16篇 |
2018年 | 15篇 |
2017年 | 9篇 |
2016年 | 8篇 |
2015年 | 16篇 |
2014年 | 13篇 |
2013年 | 23篇 |
2012年 | 36篇 |
2011年 | 33篇 |
2010年 | 14篇 |
2009年 | 15篇 |
2008年 | 14篇 |
2007年 | 20篇 |
2006年 | 23篇 |
2005年 | 21篇 |
2004年 | 31篇 |
2003年 | 27篇 |
2002年 | 30篇 |
2001年 | 23篇 |
2000年 | 30篇 |
1999年 | 24篇 |
1998年 | 8篇 |
1997年 | 4篇 |
1996年 | 9篇 |
1995年 | 3篇 |
1993年 | 2篇 |
1992年 | 18篇 |
1991年 | 15篇 |
1990年 | 6篇 |
1989年 | 12篇 |
1988年 | 11篇 |
1987年 | 7篇 |
1986年 | 7篇 |
1984年 | 2篇 |
1983年 | 3篇 |
1980年 | 2篇 |
1978年 | 3篇 |
1976年 | 3篇 |
1975年 | 3篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1970年 | 2篇 |
1968年 | 3篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1965年 | 2篇 |
排序方式: 共有628条查询结果,搜索用时 15 毫秒
1.
Dorfin localizes to the ubiquitylated inclusions in Parkinson's disease,dementia with Lewy bodies,multiple system atrophy,and amyotrophic lateral sclerosis 下载免费PDF全文
Hishikawa N Niwa J Doyu M Ito T Ishigaki S Hashizume Y Sobue G 《The American journal of pathology》2003,163(2):609-619
In many neurodegenerative diseases, the cytopathological hallmark is the presence of ubiquitylated inclusions consisting of insoluble protein aggregates. Lewy bodies in Parkinson's disease and dementia with Lewy bodies disease, glial cell inclusions in multiple system atrophy, and hyaline inclusions in amyotrophic lateral sclerosis (ALS) are representative of these inclusions. The elucidation of the components of these inclusions and the mechanisms underlying inclusion formation is important in uncovering the pathogenesis of these disorders. We hypothesized that Dorfin, a perinuclearly located E3 ubiquitin ligase, participates in the formation of ubiquitylated inclusions in a wide range of neurodegenerative diseases. Here, we report that affinity-purified anti-Dorfin antibody labeled ubiquitylated inclusions of Parkinson's disease, dementia with Lewy bodies disease, multiple system atrophy, and sporadic and familial ALS. A double-immunofluorescence study revealed that Dorfin shows a distribution pattern parallel to that of ubiquitin. Furthermore, by a filter trap assay, we detected that Dorfin is present in the ubiquitylated high-molecular weight structures derived from these diseases. These results suggest that Dorfin plays a crucial role in the formation of ubiquitylated inclusions of alpha-synucleinopathy and ALS. However, because we failed to show the direct binding of alpha-synuclein with Dorfin, future investigations into the binding partner(s) of Dorfin will be needed to deepen our understanding of the pathophysiology of alpha-synucleinopathy and ALS. 相似文献
2.
MR imaging of the cochlear modiolus: area measurement in healthy subjects and in patients with a large endolymphatic duct and sac 总被引:6,自引:0,他引:6
Naganawa S Ito T Iwayama E Fukatsu H Ishigaki T Nakashima T Ichinose N 《Radiology》1999,213(3):819-823
PURPOSE: To evaluate the cochlear modiolus with thin-section magnetic resonance (MR) imaging in healthy subjects and patients with a large endolymphatic duct and sac, and to assess whether the cochlea is normal or abnormal in patients with a large endolymphatic duct and sac. MATERIALS AND METHODS: MR images were obtained in 10 ears in five volunteers (group 1), 40 ears in 20 patients with bilateral sensory hearing loss (group 2), three ears in two patients with Mondini malformation (group 3), and 12 ears in seven patients with a large endolymphatic duct and sac (group 4). RESULTS: In groups 1 and 2, all modiolar areas were larger than 4.0 mm2. In group 3, each modiolus was smaller than 2.0 mm2. In group 4, modiolar areas were smaller than 2.0 mm2 in eight ears and were larger than 4.0 mm2 in four ears. CONCLUSION: Findings in this study confirm that a large endolymphatic duct and sac is frequently associated with modiolar deficiency, but the modiolar area is normal in some cases. This result does not support the recently proposed hypothesis that hearing loss with a large endolymphatic duct and sac is caused by the transmission of subarachnoid pressure forces into the labyrinth through a deficient modiolus. 相似文献
3.
Lung deflation impairs alveolar epithelial fluid transport in ischemic rabbit and rat lungs 总被引:3,自引:0,他引:3
Sakuma T Tsukano C Ishigaki M Nambu Y Osanai K Toga H Takahashi K Ohya N Kurihara T Nishio M Matthay MA 《Transplantation》2000,69(9):1785-1793
BACKGROUND: Because the fluid transport capacity of the alveolar epithelium after lung ischemia with and without lung deflation has not been well studied, we carried out experimental studies to determine the effect of lung deflation on alveolar fluid clearance. METHODS: After 1 or 2 hr of ischemia, we measured alveolar fluid clearance using 125I-albumin and Evans blue-labeled albumin concentrations in in vivo rabbit lungs in the presence of pulmonary blood flow and in ex vivo rat lungs in the absence of any pulmonary perfusion, respectively. RESULTS: The principal results were: (1) lung deflation decreased alveolar fluid clearance while inflation of the lungs during ischemia preserved alveolar fluid clearance in both in vivo and ex vivo studies; (2) alveolar fluid clearance was normal in the rat lungs inflated with nitrogen (thus, alveolar gas composition did not affect alveolar fluid clearance); (3) amiloride-dependent alveolar fluid clearance was preserved when the lungs were inflated during ischemia; (4) terbutaline-simulated alveolar fluid clearance was preserved in the hypoxic rat lungs inflated with nitrogen; (5) lecithinized superoxide dismutase, a scavenger of superoxide anion, and N(omega)-nitro-L-arginine methyl ester, an inhibitor of nitric oxide, preserved normal alveolar fluid clearance in the deflated rat lungs. CONCLUSION: Lung deflation decreases alveolar fluid clearance by superoxide anion- and nitric oxide-dependent mechanisms. 相似文献
4.
Shibahara E Fukatsu H Naganawa S Ito T Iwayama E Ishigaki T Segawa T Zhang W 《Nagoya journal of medical science》2000,63(1-2):41-49
In this paper, we tried to evaluate the effect of water-fat separation on and to optimize the scan condition of the newly developed "Sandwiched" 3-point Dixon method at 0.35 Tesla (T), for knee joint imaging. Using a 0.35T superconductive open magnet system with a solenoid type knee coil, one male and two female normal volunteers (27-37 y.o.) underwent knee joint imaging. Each sequence provided good water-fat separated images. At 0.35T, the gradient echo provided a better contrast than the spin echo. Optimal cartilage-marrow and cartilage-fluid contrast could be obtained at a frip angle (FA) of 90 degrees. There was no significant correlation between cartilage-marrow, cartilage-fluid contrast and repetition time (TR) values within the tested range. Cartilage-fluid and cartilage-marrow contrast were both best at an FA of 90 degrees with the gradient echo sequence. TR from 350 ms to 650 ms did not cause any significant contrast difference in the fat suppressed images. This method is useful and could be the only practical choice for obtaining fat suppressed T1 weighted images for joint magnetic resonance (MR) imaging at 0.35T. 相似文献
5.
6.
Naro Ohashi Hiroyuki Takase Taro Aoki Takashi Matsuyama Sayaka Ishigaki Shinsuke Isobe Tomoyuki Fujikura Akihiko Kato Hideo Yasuda 《Medicine》2021,100(19)
Excessive salt intake causes hypertension and cardiovascular diseases (CVDs). B-type natriuretic peptide (BNP) is synthesized and released from the ventricle, and is a surrogate marker reflecting various CVDs. Moreover, when a slight BNP elevation is shown, it leads to a poor prognosis in the general population. However, the relationship between salt intake and BNP levels in the general population remains unclear, especially in those without hypertension and heart diseases.In this study, we recruited 1404 participants without hypertension and electrocardiogram abnormalities, who received regular annual health check-ups in Japan. Plasma BNP levels were measured, and daily salt intake levels were evaluated using urinary samples. In addition, some clinical parameters were obtained, and the data were cross-sectionally analyzed.The median of plasma BNP levels was 10.50 pg/mL, and daily salt intake was 8.50 ± 1.85 g. When dividing participants into quartiles according to daily salt intake, those with the highest daily salt intake revealed the highest plasma BNP levels. Plasma BNP levels were significantly and positively associated with daily salt intake. Moreover, multiple linear regression analyses revealed that plasma BNP levels showed a significant positive association with daily salt intake levels after adjustments.Plasma BNP levels were significantly and positively associated with daily salt intake after adjustment in the general population. Plasma BNP levels may be a surrogate marker reflecting salt-induced heart diseases. 相似文献
7.
8.
9.
Antibiotic prophylaxis for endoscopic retrograde chlangiopancreatography increases the detection rate of drug‐resistant bacteria in bile 下载免费PDF全文
10.