粉防已碱抗炎作用机理的研究

粉防已碱(Tetrandrine,Tet)是从中药粉防已中提出的有效成分,单体为双苄基异喹啉衍生物,具有镇痛,消炎,抗风湿,抗过敏,抗心律失常,降压等作用,临床上已用于高血压的治疗,虽然五十年代吕富华等已证明Tet有治疗甲醛性关节炎的作用,但因条件限制,至今对其作用机制,尤其是抗炎作用机制仍不明确。为此。我们进行了一系列研究,目     

Effects of Tetrandrine on Rabbit Platelet Morphology and Aggregation in vitro

Using turbidimetry and electronmicroscopy,we examined the effects oftetrandrine (Tet) on the aggregation and morphological changes of rabbit platelets.It wasfound that Tet could markedly inhibit the platelet aggregation induced by ADP,collagenand arachidonic acid (AA) in a dose-dependent manner.The IC_(50) (50% inhibitoryconcentration) was 38.9,30.3 and 44.3μg/ml respectively.The platelets pretreated with Tetcould not be activated by collagen,their morphological changes and granule release didn'toccur and their normal structure was well preserved.In addition,dipyridamole couldenhance the inhibition of Tet on platelet aggregation,while increased extracellular calciumcould weaken this inhibition.     

成人中肠旋转异常二例

中肠旋转异常婴幼儿多见 ,而成人罕见。笔者遇见 2例报告如下。例 1　男 ,5 3岁。恶心、呕吐 1月余。近来呕吐呈喷射状 ,伴上腹部疼痛 ,吐出草绿色水样物。胃镜见胃底、胃体糜烂性胃炎 ,病理诊断为胃多发性小溃疡。立位腹部平片见上腹部双泡征 ,中下腹少量肠腔积气。钡餐检查见胃、十二指肠明显扩张 ,十二指肠水平段远端梗阻 ,粘膜纹呈锥状 ,肠管径越来越细 ,考虑胃、十二指肠扩张 ,肠梗阻、先天畸形可能 (图 1、2 )。手术见胃、十二指肠水平段屈氏韧带处 (Ｔｒｅｉｔｚ)小肠系膜根部 180°扭转 ,回盲部阑尾位于右上腹 ,行肠系膜粘连剥离 ,…     

粉防己碱对Bay k 8644引起兔离体主动脉条收缩的抑制作用

In the presence of KCl 19 mmol.L-1, calcium agonist Bay k 8644 0.47 mumol.L-1 elicited a strong contraction of isolated rabbit aortic strips, and this contraction was concentration-dependently inhibited by tetrandrine; but this antagonism was noncompetitive. Calcium ionophore calcimycin evoked contraction was markedly depressed by tetrandrine. The results suggested that tetrandrine might not only inhibit transmembrane influx of calcium via potential-dependent channels but also interfere with other processes related to calcium.     

粉防己碱对兔血小板的作用及其机制探讨

实验发现Tet明显抑制ADP、胶原和花生四烯酸(AA)诱导的血小板聚集其,效应呈剂量依赖性,Tet对这三种诱导剂诱导聚集的IC_(50)。分别为38.9,30.3和44.3ug/ml;增加细胞外钙可部分抵消Tet抑制血小板的效应,而与藩生丁联合使用则加强Tet的抑制效应,产生明显的协同作用;经Tet处理的血小板,胶原刺激可能引起形态改变,Tet保护血小板正常     

槐果碱及阿斯匹林对正常大鼠血浆PGI_2和TXA_2水平的影响

槐果碱可明显升高正常大鼠血浆6-酮-PGF_(1a)含量,对TXB_2无明显影响。小剂量(1mg/100g)阿斯匹林轻度降低血浆6-酮-PGF_(1a)含量,但明显阻断槐果碱升高6-酮-PGF_(1a)的作用。     

血小板钙与磷脂代谢的相互作用

血小板激动剂大多数不能透过血小板膜,虽然其种类不同,但它们所诱导的血小板反应却十分相似,因此,推测它们在诱导血小板反应时,必定有一些共同的途径。血小板在受到激动剂刺激后,经历形态变化、聚集、释放,在聚集的后期阶段释放出花生四     

三氟啦嗪对胶原和ADP诱导血小板合成TXA_2的不同影响——提示花生四稀酸释放机理不同

血小板内只含有少量游离的花生四烯酸(AA),而多数却连接在磷脂上,当血小板受到诱导剂刺激时,AA则从膜磷脂上释放出来。已知AA释放可能涉及到三个不同的机理。不同的诱导剂在诱导AA释放时,其途径亦可能不尽相同。本文通过测定AA环氧酶途径代谢产物—TXB_2,比较了胶原和ADP诱导AA释放的差异。     

粉防己碱对兔血小板聚集及形态的影响

粉防己碱(Tet)在体外明显抑制ADP、胶原和花生四烯酸诱导的兔血小板聚集,其效应呈剂量依赖性。对胶原诱导的血小板形态改变及颗粒释放也有明显抑制作用。Tet与潘生丁伍用能明显加强它们对血小板聚集的抑制作用,而增加细胞外钙可减弱Tet对血小板聚集的抑制作用。