米非司酮联合依沙吖啶终止中期妊娠的临床观察

目的:观察米非司酮联合依沙吖啶终止中期妊娠的临床效果及安全性。方法:选择16~26周妊娠要求终止妊娠的妇女200例,随机分成观察组和对照组。两组均经腹壁羊膜腔注射依沙吖啶100mg,观察组同时口服米非司酮50mg,每间隔12h1次,总量为150mg。观察两组引产效果及安全性。结果:两组引产成功率均为100%;观察组注药至宫缩、宫缩至胎儿娩出时间均明显短于对照组(P<0.01);产后2h出血量及胎盘、胎膜残留率明显低于对照组(P<0·05);观察组仅有轻度恶心及头昏等副反应,对照组无明显副反应。结论:米非司酮联合依沙吖啶终止中期妊娠引产时间短、产后出血少、清宫率低,是安全、有效的方法。     

血清蛋白电泳69例异常结果分析

常用的醋酸纤维薄膜电泳技术可将血清蛋白分为白蛋白、α1球蛋白、α2球蛋白、β球蛋白、γ球蛋白,每个区带都含有一种或多种蛋白质成分,各区带的变化与疾病间有密切的关系。不少学者将其分为11种谱型:低蛋白血症型、肾病型、肝硬化型、急性炎症或急性时相反应型、慢性炎症型、弥漫性肝损害型、弥散宽γ球蛋白型、M蛋白血症型、高α2(β)球蛋白血症型、妊娠型(高α1)、蛋白质缺陷型[1,2]。为此,我们对我院2002~2004年间蛋白电泳69例异常结果进行分析,旨在为临床常见疾病的诊断与治疗提供参考。1临床资料1·1一般情况本组共69例作为观察组,…     

儿童术后疼痛评估软件的设计与应用

目的开发高效、准确及受患儿欢迎的疼痛评估工具,提高疼痛评估水平。方法在Windows环境下,采用Visual Basic6.0语言编程,建立包括软件说明、信息录入、疼痛评估、信息查询、用户管理5个功能模块的儿童疼痛评估软件;与Wong-Baker面部表情量表同时应用于80例手术患儿术后至第3天于静息、床上活动、进食、深呼吸、咳嗽状态下的疼痛评估。结果两种方法疼痛5项分值的相关系数r为0.630～0.872,93.75%患儿首选疼痛评估软件为自己评估疼痛。结论儿童疼痛评估软件性能好,用于评估术后患儿疼痛效果可靠,且受患儿欢迎。     

抗氧化剂对博莱霉素拮抗作用的研究

目的观察抗氧化剂（维生素E、牛黄酸、二甲亚砜和维生素C）针对博莱霉素（Bleomycin,BLM）的拮抗作用.方法在人外周血淋巴细胞培养物中注入一定剂量的BLM或并一定剂量的抗氧化剂,然后观察淋巴细胞的姐妹染色单体交换率（sister chromatid exchanges ,SCE率）的变化.结果维生素E浓度为5 μl/ml时,与28.6 nmol/ml的BLM处理组（对照2）相比,可显著地拮抗BLM的作用（P〈0.05）;而维生素E浓度为10 μl/ml时,可极显著地拮抗BLM的作用（P〈0.01）.当加入牛黄酸的终浓度为100和200 μg/ml时,与28.6 nmol/ml的BLM处理组（对照2）相比,均可显著地拮抗BLM的作用（P〈0.05）.结论一定剂量的维生素E和牛黄酸对BLM存在着拮抗作用.     

周仲瑛用化肝解毒汤治疗乙型肝炎经验

周仲瑛,生于1928年,江苏省如东县人,是首批国家级名老中医,现为南京中医学院教授兼任卫生部药品审评委委员,国家教委科技委医药卫生学科组组员等职。家世业医,少承庭训,著有《中医内科学》等十余种专著。临床40余年,对中医内科和急症造诣殊深,尤其对乙型肝炎的诊治有丰富经验,现作一简要阐述。     

西宁地区冠心病人血液粘滞性改变的初步观察

本文对51例冠心病患者血液流变学的指标进行了测定,结果显示:冠心病患者的血液流变学具有浓(浓稠性)、粘(粘滞性)、聚(聚集性)、凝(凝固性)等特征,这与平原报道资料一致。并通过血液流变学的改变,初步探讨了西宁地区冠心病发病率低的原因。     

Effect of thyroid hormone level on the expression of synaptotagmin Ⅰ in adult rat hippocampus

Objective To observe the effect of different thyroid hormone level on the expression of synaptotagmin Ⅰ(Syt Ⅰ) in adult rat hippocampus. Methods All 28 adult male SD rats were assigned randomly into hypothyroid, hyperthyroid and control group, hypothyroid group was established by daily intraperitoneal injections with propylthiou raci(PTU, 10.0 mg/kg body weight) for 6 weeks and hyperthyroid group with L-Thyroxine (L-T4, 0.5 mg/kg body weight) for 3 weeks. Radioimmunity method was used to assay the levels of serum T3 and T4, immunohistochemical S-P technology to assay the levels of Syt Ⅰ protein in hippoeampus CA1, CA3 and dentate gyrus (DG). The layers analyzed in the different subfields include the polymorphic cell layer(the stratum oriens, SO), pyramidal cell layer(PCL), stratum radiatum (SR), lacunosum-molecular layer (SLM) in CA1 and CA3, granular cell layer(GL) and molecular layer(ML) in DG. Results The levels of serum T3 and T4[(0.34±0.12), (41.03± 11.37)nmol/L]in the hypothyroid rats were significantly lower than those in the control group[(0.65±0.15), (55.20±10.68)nmol/L, P < 0.01 or < 0.05], and the positive granule of Syt Ⅰ was significantly lower in PCL and SR of CA1 and CA3, GL of DG. The average optical value responsible for Syt Ⅰ immunoreactivity was obviously reduced in SO(0.048±0.007), PCL(0.299±0.035), SR(0.042±0.007), SLM(0.038±0.006) of CA1, PCL(0.085± 0.019), SR(0.040±0.011), SLM (0.038±0.006) of CA3, GL (0.076±0.019) of DG than normal controls (0.068± 0.014, 0.376±0.053, 0.053±0.008,0.056±0.009,0.118±0.026,0.052±0.010,0.053±0.009,0.099±0.015; P< 0.01 or < 0.05). Serum T3 and T4 levels [(1.43±0.30), (157.18±19.95)nmol/L]of hyperthyroid rats were significantly higher than those of control group(P < 0.01). The value was reduced in PCL(0.322±0.050), SR(0.039±0.006), SLM (0.042±0.006) of CA1, PCL(0.098±0.034), SR(0.046±0.013), SLM(0.046±0.010) of CA3 and GL(0.085± 0.024), ML (0.042±0.009) of DG (P < 0.05 or < 0.01). Conclusion Adult-onset of hypothyroidism and hyperthyroidism can reversibly decrease the expression of Syt Ⅰ in CA1, CA3 and DG regions of hippocampus.     

Effect of thyroid hormone level on the expression of synaptotagmin Ⅰ in adult rat hippocampus

Objective To observe the effect of different thyroid hormone level on the expression of synaptotagmin Ⅰ(Syt Ⅰ) in adult rat hippocampus. Methods All 28 adult male SD rats were assigned randomly into hypothyroid, hyperthyroid and control group, hypothyroid group was established by daily intraperitoneal injections with propylthiou raci(PTU, 10.0 mg/kg body weight) for 6 weeks and hyperthyroid group with L-Thyroxine (L-T4, 0.5 mg/kg body weight) for 3 weeks. Radioimmunity method was used to assay the levels of serum T3 and T4, immunohistochemical S-P technology to assay the levels of Syt Ⅰ protein in hippoeampus CA1, CA3 and dentate gyrus (DG). The layers analyzed in the different subfields include the polymorphic cell layer(the stratum oriens, SO), pyramidal cell layer(PCL), stratum radiatum (SR), lacunosum-molecular layer (SLM) in CA1 and CA3, granular cell layer(GL) and molecular layer(ML) in DG. Results The levels of serum T3 and T4[(0.34±0.12), (41.03± 11.37)nmol/L]in the hypothyroid rats were significantly lower than those in the control group[(0.65±0.15), (55.20±10.68)nmol/L, P < 0.01 or < 0.05], and the positive granule of Syt Ⅰ was significantly lower in PCL and SR of CA1 and CA3, GL of DG. The average optical value responsible for Syt Ⅰ immunoreactivity was obviously reduced in SO(0.048±0.007), PCL(0.299±0.035), SR(0.042±0.007), SLM(0.038±0.006) of CA1, PCL(0.085± 0.019), SR(0.040±0.011), SLM (0.038±0.006) of CA3, GL (0.076±0.019) of DG than normal controls (0.068± 0.014, 0.376±0.053, 0.053±0.008,0.056±0.009,0.118±0.026,0.052±0.010,0.053±0.009,0.099±0.015; P< 0.01 or < 0.05). Serum T3 and T4 levels [(1.43±0.30), (157.18±19.95)nmol/L]of hyperthyroid rats were significantly higher than those of control group(P < 0.01). The value was reduced in PCL(0.322±0.050), SR(0.039±0.006), SLM (0.042±0.006) of CA1, PCL(0.098±0.034), SR(0.046±0.013), SLM(0.046±0.010) of CA3 and GL(0.085± 0.024), ML (0.042±0.009) of DG (P < 0.05 or < 0.01). Conclusion Adult-onset of hypothyroidism and hyperthyroidism can reversibly decrease the expression of Syt Ⅰ in CA1, CA3 and DG regions of hippocampus.     

Effect of thyroid hormone level on the expression of synaptotagmin Ⅰ in adult rat hippocampus

Objective To observe the effect of different thyroid hormone level on the expression of synaptotagmin Ⅰ(Syt Ⅰ) in adult rat hippocampus. Methods All 28 adult male SD rats were assigned randomly into hypothyroid, hyperthyroid and control group, hypothyroid group was established by daily intraperitoneal injections with propylthiou raci(PTU, 10.0 mg/kg body weight) for 6 weeks and hyperthyroid group with L-Thyroxine (L-T4, 0.5 mg/kg body weight) for 3 weeks. Radioimmunity method was used to assay the levels of serum T3 and T4, immunohistochemical S-P technology to assay the levels of Syt Ⅰ protein in hippoeampus CA1, CA3 and dentate gyrus (DG). The layers analyzed in the different subfields include the polymorphic cell layer(the stratum oriens, SO), pyramidal cell layer(PCL), stratum radiatum (SR), lacunosum-molecular layer (SLM) in CA1 and CA3, granular cell layer(GL) and molecular layer(ML) in DG. Results The levels of serum T3 and T4[(0.34±0.12), (41.03± 11.37)nmol/L]in the hypothyroid rats were significantly lower than those in the control group[(0.65±0.15), (55.20±10.68)nmol/L, P < 0.01 or < 0.05], and the positive granule of Syt Ⅰ was significantly lower in PCL and SR of CA1 and CA3, GL of DG. The average optical value responsible for Syt Ⅰ immunoreactivity was obviously reduced in SO(0.048±0.007), PCL(0.299±0.035), SR(0.042±0.007), SLM(0.038±0.006) of CA1, PCL(0.085± 0.019), SR(0.040±0.011), SLM (0.038±0.006) of CA3, GL (0.076±0.019) of DG than normal controls (0.068± 0.014, 0.376±0.053, 0.053±0.008,0.056±0.009,0.118±0.026,0.052±0.010,0.053±0.009,0.099±0.015; P< 0.01 or < 0.05). Serum T3 and T4 levels [(1.43±0.30), (157.18±19.95)nmol/L]of hyperthyroid rats were significantly higher than those of control group(P < 0.01). The value was reduced in PCL(0.322±0.050), SR(0.039±0.006), SLM (0.042±0.006) of CA1, PCL(0.098±0.034), SR(0.046±0.013), SLM(0.046±0.010) of CA3 and GL(0.085± 0.024), ML (0.042±0.009) of DG (P < 0.05 or < 0.01). Conclusion Adult-onset of hypothyroidism and hyperthyroidism can reversibly decrease the expression of Syt Ⅰ in CA1, CA3 and DG regions of hippocampus.