超声造影增强模式鉴别软组织肿块良恶性

Objective: To investigate the ability of contrast enhancement patterns of contrast-enhanced ultrasound in differentiating benign and malignant soft tissue tumours.     

Three-dimensional biomechanical properties of the human rectum evaluated with magnetic resonance imaging

A method to evaluate the three-dimensional (3-D) geometry of the human gastrointestinal wall may be valuable for understanding tissue biomechanics, mechano-sensation and function. In this paper we present a magnetic resonance imaging (MRI) based method to determine rectal geometry and validation of data obtained in three volunteers. A specially designed rectal bag was filled in a stepwise manner while MRI and bag pressure were recorded. 3-D models of curvatures, radii of curvature, tension and stress were generated and the circumferential and longitudinal strains were calculated. The computed bag volumes corresponded to the infused volumes. A pronounced bag elongation and decrease in wall thickness was observed during the bag filling. The spatial distributions of the biomechanical parameters were distinctly different between individuals and non-homogeneous throughout the rectal wall due to its complex geometry. The average tension and stress increased as a function of infused volume and circumferential strain. The present study provides a method for characterizing the complex in vivo 3-D geometry of the human rectum. The non-homogenous spatial curvature distribution suggests that simple estimates of tension based on pressure and volume do not reflect the true 3-D biomechanical properties of the rectum.     

强迫体位下经皮椎体成形术治疗腰椎转移性肿瘤疗效观察

目的 探讨强迫体位下行PVP手术的手术技巧及疗效观察;方法：综述特殊体位下PVP手术经验,结合一例强迫体位下经皮椎体成形术治疗腰椎转移性肿瘤病例总结学习强迫体位下行PVP手术的手术技巧及疗效观察;结果：强迫性体位下PVP手术对手术医生要求更高,而疗效与常规俯卧位下PVP手术相当;     

小型猪肝硬化模型肝脏病理及生化指标的变化

目的 探讨小型猪肝硬化模型制备过程中肝脏病理学及生化指标的变化。方法 选用同种系巴马小型猪50头,随机分为实验组40头,对照组10头,雌雄不限,4月龄,体重15-20kg。实验组予四氯化碳复合因素造模法喂养,正常组予饲料及清水喂养。于造模的0、4、8、12、16、20、24周末进行肝脏穿刺活检及耳缘静脉抽血进行数据采集,对比分析肝纤维化-肝硬化制模过程中的肝脏病理学及生化指标的变化。结果24周末,对照组获得数据S0期70例次;实验组获得数据轻度肝纤维化（S1＋S2期）82例次,重度肝纤维化（S3＋S4期）62例次,肝硬化共39例次。从正常组到肝硬化组谷丙转氨酶、谷草转氨酶、总胆红素、血清肌酐及球蛋白呈逐渐升高的趋势,而白蛋白,A/G比值呈逐渐下降的趋势,凝血酶原时间则逐渐延长,统计学分析,组间存在明显差异（P＜0.01）。结论 四氯化碳复合因素造模法可以成功制备出小型猪肝硬化模型,肝纤维化不同阶段肝脏病理学和生化指标均发生明显变化。     

应用Dot—ELISA检测过敏性皮肤病患者过敏原的研究

应用Dot-ELISA检测过敏性皮肤病患者血清对多种过敏原的特异性IgG抗体 ,了解过敏原对患者的致敏状况 ,并研究该试验的最适条件 ,检测方法的敏感性和重复性。结果表明Dot-ELISA检测过敏原和抗过敏原的特异性IgG抗体具较高特异性和敏感性 ,且具成本低 ,操作简便 ,观测结果直接等优点。为检测过敏性皮肤病患者过敏原的一种较好方法。     

人成骨细胞增殖和分化与生长激素释放肽☆

背景：有研究表明生长激素释放肽对体外培养大鼠成骨细胞增殖有促进作用,但对人成骨细胞增殖和分化是否有影响却少有报道。 目的：探讨生长激素释放肽对人成骨细胞增殖和分化的影响。 方法：取正常成人髂前上棘松质骨,分离出成骨细胞并进行体外培养,应用RT-PCR法检测生长激素促分泌素受体的表达;3H-掺入法检测成骨细胞增殖;Westernblot检测与成骨细胞分化相关的Runx2蛋白表达;α-磷酸奈酚法和放射免疫法观察生长激素释放肽对人成骨细胞分化的影响。 结果与结论：人成骨细胞可表达生长激素促分泌素受体mRNA和生长激素促分泌素受体蛋白。生长激素释放肽促进人成骨细胞增殖(P < 0.05或P < 0.01),且呈剂量依赖性,而且可增加碱性磷酸酶的活性并促进骨钙素的分泌(P < 0.05或P < 0.01),但对人成骨细胞中Runx2蛋白的表达无影响。结果提示,生长激素释放肽能促进人成骨细胞增殖和分化。 关键词：生长激素释放肽;人成骨细胞;细胞增殖;细胞分化;组织构建     

癫痫、抗癫痫药与男性病人性激素的相互关系

Objective To explore the relationship between antiepileptic drugs(AED)and hormones of male epilep-tics.Methods:Comparison of 13 male epileptics before and after taking AED.Radioimmunoassay was taken totest total testosterone(TT),follicli-stimulation hormone(FSH),luteinising hormone(LH),prolactin(PRL),percentage of free testosterone(FT%),free testosterone(FTpmol/1),sex hormone binding globulin(SHBG).Result:After the treatment,TT,FT(pmol/1 were increased significantly,PRL was decreased definitely.LH、FSH、FT%、SHBG were of no significant differences.Conclusion:AED had some effects on the hormones of thepatients and epilepsy had no direct relationship with the sex hormones of the patients.     

Modelling the elastin, collagen and smooth muscle contribution to the duodenal mechanical behaviour in patients with systemic sclerosis

Abstract  Systemic sclerosis (SS) is a connective tissue disease that involves the gastrointestinal tract. Previous experiments have shown abnormal intestinal motility, dilatation, wall stiffening and impaired smooth muscle function. Consequently, understanding the association between intestinal wall mechanics, structure and function is important. The aim was to establish a model for differentiating the biomechanical remodelling of elastin, collagen and smooth muscle in the duodenum in SS patients. A duodenal distension protocol was used in six patients and five healthy controls. A theoretical model for evaluating the mechanical contributions of elastin, collagen and smooth muscle tone was established. The tension–strain curves computed from pressure and cross-sectional area data were analysed. The elastic modulus of elastin, the relationship between the collagen recruitment, collagen density and the active tension were calculated. The model fitted the clinical data well. The material constant for elastin in the patients was 30% lower than in the control group ( P  =   0.005). More collagen was recruited in patients than in healthy volunteers. Eighty percent of the collagen fibres were recruited at stretch ratio 0.85–2.26 (1.61 averaged) in patients and at the stretch ratio 2.55–3.73 (2.97 averaged) in healthy controls. The maximum active muscle tension and the corresponding strain were lowest in the patients ( P  =   0.01). The model can be used to determine the contribution of tissue components to the mechanical behaviour of duodenum. The stiffer wall in patient was due to the small stretch ratio for the maximum collagen recruitment but the muscle activity was also impaired.     

鸡形觉剥夺性近视眼视网膜光镜下形态改变

To explore the morphological changes in form-deprived chickens’ retinas,24 neonatal chickens underwent monocular goggle occlusion．The retinas were observed under light microscopy at different stages of occlusion．We found that the deprived retinas were significantly thinner than those of the control eyes,especially in the inner nuclear and inner plexiform layers,while there were no much difference in the thickness of the photoreceptor layers of the two groups．Thus,we conclud that at the level of light microscopy,the inner part of retina manifests significant changes firstly．     

Geometric and mechanosensory properties of the sigmoid colon evaluated with magnetic resonance imaging

The aim of this study was to use magnetic resonance imaging (MRI) to evaluate the three-dimensional geometry and mechanosensory properties of the sigmoid colon. The sigmoid colon was stepwise distended by a water-filled bag in eight subjects. Simultaneous MRI, bag pressure recording and sensory assessment were performed before and after smooth muscle relaxation with butylscopolamine. The surface distributions of principal curvature radii, wall thickness, tension, stress and circumferential strain were calculated. The geometry of the distended sigmoid colon was complex and the spatial distributions of the biomechanical parameters were non-homogeneous. The circumferential length, strain, pressure and wall stress increased as a function of bag volume (all P < 0.001). In response to butylscopolamine, the pressure and wall stress were reduced (P < 0.05) and the stress-strain curves were shifted to the right. The sensory response was a linear function of the biomechanical parameters (all P < 0.001) and decreased in response to butylscopolamine as a function of volume (P = 0.02). The stimulus-response data indicate that the mechanosensitive afferents are affected by smooth muscle tone. The present study provides a method for characterizing the complex geometry and mechanical properties of the sigmoid colon, including the role of smooth muscle tone. This may be valuable in understanding of the biomechanical and mechanosensory functions in colonic diseases.