全文获取类型
收费全文 | 358篇 |
免费 | 13篇 |
国内免费 | 2篇 |
专业分类
儿科学 | 3篇 |
妇产科学 | 3篇 |
基础医学 | 35篇 |
口腔科学 | 1篇 |
临床医学 | 105篇 |
内科学 | 112篇 |
皮肤病学 | 2篇 |
神经病学 | 47篇 |
特种医学 | 1篇 |
外科学 | 27篇 |
综合类 | 5篇 |
预防医学 | 16篇 |
眼科学 | 1篇 |
药学 | 11篇 |
肿瘤学 | 4篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 8篇 |
2020年 | 3篇 |
2019年 | 9篇 |
2018年 | 7篇 |
2017年 | 5篇 |
2016年 | 4篇 |
2015年 | 5篇 |
2014年 | 14篇 |
2013年 | 10篇 |
2012年 | 26篇 |
2011年 | 27篇 |
2010年 | 23篇 |
2009年 | 19篇 |
2008年 | 38篇 |
2007年 | 23篇 |
2006年 | 27篇 |
2005年 | 22篇 |
2004年 | 19篇 |
2003年 | 9篇 |
2002年 | 14篇 |
2001年 | 14篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1973年 | 2篇 |
1966年 | 1篇 |
1965年 | 2篇 |
排序方式: 共有373条查询结果,搜索用时 15 毫秒
1.
Evaluation of the Du Pont HERPCHEK herpes simplex virus antigen test with clinical specimens. 总被引:1,自引:4,他引:1
The HERPCHEK herpes simplex virus (HSV) antigen test (Du Pont, Billerica, Mass.) is a biotinstreptavidin-amplified enzyme-linked immunoassay for the detection of HSV antigen directly in clinical specimens. In a total of 200 mucocutaneous specimens, predominantly lesions from nongenital sites, HERPCHEK had a sensitivity of 90% and a specificity of 99% compared with the shell vial tissue culture method which uses primary rabbit kidney cells. Five false-negative results by HERPCHEK were found with specimens that contained a low percentage of positive cells by the direct immunoperoxidase stain used in a tissue culture method (1 to 9%). The absorbance values of the clinical specimens by the HERPCHEK test do not correlate well with the percentage of positive cells. Our results indicate that the HERPCHEK HSV antigen test, when performed with specimens from lesions, is a sensitive and specific test as compared with shell vial culture. 相似文献
2.
Ramaz Katsarava Tamara Kartvelishvili Tamara Khosruashvili Vakhtang Beridze 《Macromolecular chemistry and physics.》1995,196(9):3061-3074
A new synthesis pathway to polyurethanes (PURs) by polycondensation of active carbonates of diols with hexamethylenediamine was investigated in detail including model studies. It has been shown that high-molecular-weight PURs (ηred ≤ 3,8 dL/g) can be obtained under optimum reaction conditions. Salts of the diamine and its N,N′-trimethyl-silylated derivative were also used for the synthesis of film- and fiber-forming PURs. 相似文献
3.
Joseph F. Clark Zaza Khuchua Ernest Boehm Renée Ventura-Clapier 《Journal of muscle research and cell motility》1994,15(4):432-439
Summary Activity and role of creatine kinase associated with contractile proteins of vascular smooth muscle have been investigated using skinned guinea-pig carotid artery rings. Membrane solubilization was performed with the detergent Triton X-100. Creatine kinase activity, isoenzyme profile as well as mechanics were performed on the Triton skinned carotid artery rings. Total creatine kinase activity was 47.3±9.3 IU g1 ww and electrophoresis showed BB, MB, and MM isoforms (BB-CK being the predominant isoenzyme). One hour incubation with Triton X-100, produced predominantly BB-CK remaining with the myofibrils with some MB, representing 23% of the preskinned creatine activity. When relaxed carotid artery rings were exposed to pCa 9 in the presence of 250 M ADP, 0 ATP, and 12 mM phosphocreatine, tension was not significantly different from resting tension, but changing to pCa 4.5 caused the carotid artery rings to generate 49.5±4.5% of maximal tension. When a high-tension rigor state was achieved (250 M ADP, 0 ATP, 0 phosphocreatine, and pCa 9), the addition of 12 mM phosphocreatine effected significant relaxation. These observations implicate an endogenous form of creatine kinase, associated with the myofilaments, which is capable of producing enough ATP for submaximal tension generation and significant relaxation from rigor conditions. These results suggest co-localization of ATPase, MLCK, and creatine kinase on the contractile proteins of the carotid artery. Such an enzymic association may play a role in the energetic supply to the contractile apparatus of vascular smooth muscle.Recipient of INSERM/NIH Fogarty International Fellowship, TW01585-01. 相似文献
4.
5.
A. Zaza G. Maccaferri M. Mangoni D. DiFrancesco 《Pflügers Archiv : European journal of physiology》1991,419(6):662-664
A study on the cardiac pacemaker current if in inside-out macro-patches of sino-atrial (SA) node cells has recently demonstrated that if channels are directly activated by intracellular cAMP [4]. Using the same preparation, here we investigate the possibility that internal Ca2+ ions play a role in the modulation of if channels. Our results indicate that Ca2+ ions do not have a direct activating effect on if. 相似文献
6.
George A. Mensah Richard A. Goodman Stephanie Zaza Anthony D. Moulton Paula L. Kocher William H. Dietz Terry F. Pechacek James S. Marks 《Preventing chronic disease》2004,1(1)
Law, which is a fundamental element of effective public health policy and practice, played a crucial role in many of public health's greatest achievements of the 20th century. Still, conceptual legal frameworks for the systematic application of law to chronic disease prevention and control have not been fully recognized and used to address public health needs. Development and implementation of legal frameworks could broaden the range of effective public health strategies and provide valuable tools for the public health workforce, especially for state and local health department program managers and state and national policy makers. In an effort to expand the range of effective public health interventions, the Centers for Disease Control and Prevention will work with its partners to explore the development of systematic legal frameworks as a tool for preventing chronic diseases and addressing the growing epidemic of obesity, heart disease, stroke, and other chronic diseases and their risk factors. 相似文献
7.
Poor pain assessment is cited as one barrier to the adequate treatment of cancer pain. The identification of relevant psychosocial factors may improve the assessment of chronic cancer pain. This article presents: 1) a critical review of the evidence for an association between chronic cancer pain and psychological distress, social support, and coping; 2) clinical implications of the findings; and 3) recommendations for future research. Fourteen of the 19 reviewed studies on psychological distress found a significant association between increased pain and increased distress. Seven of the eight studies on social support found significant association between higher levels of pain and decreased levels of social activities and social support. Three of the four studies that examined coping strategies found that increased catastrophizing was significantly associated with more intense pain. Based on several criteria, the evidence is considered Strong for psychological distress, Moderate for social support, and Inconclusive for coping. This review suggests that comprehensive chronic pain assessment should include routine screening for psychological distress. 相似文献
8.
The symptom headache is very frequent. Most frequently headache is the leading symptom of a primary headache syndrome such as migraine or tension-type headache. Sometimes it is caused by another disease. In everyday clinical practice it is important to diagnose and treat primary headaches properly. It is even more important not to miss a secondary headache, which is rare, but if misdiagnosed could conceal life-threatening conditions. This review provides an overview of the clinical picture, diagnostic procedures and treatment strategies of frequent headache syndromes such as migraine, tension-type headache, medication overuse headache, trigeminal autonomic cephalgias and trigeminal neuralgia. This is followed by a brief summary on symptomatic headache caused by non-neurological diseases as well as on diagnostic procedures and management of headache in the emergency situation. 相似文献
9.
Daniele Piovani Maria Nefeli Christodoulou Andreas Hadjidemetriou Katerina Pantavou Paraskevi Zaza Pantelis G. Bagos Stefanos Bonovas Georgios K. Nikolopoulos 《The Journal of infection》2021,82(1):133-142
ObjectivesTo estimate the effect of early application of social distancing interventions on Covid-19 cumulative mortality during the first pandemic wave.MethodsEcological longitudinal study using multivariable negative binomial regression for panel data. Daily numbers of Covid-19 cases and deaths, and data on social distancing interventions, for the 37 member countries of the Organization for Economic Cooperation and Development (OECD) were analysed.ResultsCovid-19 cumulative mortality over the first pandemic wave varied widely across countries (range, 4.16 to 855 deaths per million population). On average, one-day delay in application of mass gatherings ban was associated with an adjusted increase in Covid-19 cumulative mortality by 6.97% (95% CI, 3.45 to 10.5), whilst a one-day delay in school closures was associated with an increase of 4.37% (95% CI, 1.58 to 7.17) over the study period. We estimated that if each country had enacted both interventions one week earlier, Covid-19 cumulative mortality could have been reduced by an average of 44.1% (95% CI, 20.2 to 67.9).ConclusionsEarly application of mass gatherings ban and school closures in outbreak epicentres was associated with an important reduction in Covid-19 cumulative mortality during the first pandemic wave. These findings may support policy decision making. 相似文献
10.