全文获取类型
收费全文 | 1093篇 |
免费 | 62篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 43篇 |
妇产科学 | 4篇 |
基础医学 | 116篇 |
口腔科学 | 19篇 |
临床医学 | 146篇 |
内科学 | 237篇 |
皮肤病学 | 17篇 |
神经病学 | 87篇 |
特种医学 | 158篇 |
外科学 | 87篇 |
综合类 | 52篇 |
预防医学 | 74篇 |
眼科学 | 4篇 |
药学 | 58篇 |
1篇 | |
中国医学 | 2篇 |
肿瘤学 | 53篇 |
出版年
2023年 | 3篇 |
2022年 | 2篇 |
2021年 | 11篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 15篇 |
2017年 | 13篇 |
2016年 | 19篇 |
2015年 | 43篇 |
2014年 | 61篇 |
2013年 | 47篇 |
2012年 | 50篇 |
2011年 | 63篇 |
2010年 | 42篇 |
2009年 | 40篇 |
2008年 | 52篇 |
2007年 | 63篇 |
2006年 | 54篇 |
2005年 | 48篇 |
2004年 | 38篇 |
2003年 | 27篇 |
2002年 | 30篇 |
2001年 | 30篇 |
2000年 | 33篇 |
1999年 | 30篇 |
1998年 | 21篇 |
1997年 | 17篇 |
1996年 | 22篇 |
1995年 | 23篇 |
1994年 | 32篇 |
1993年 | 19篇 |
1992年 | 20篇 |
1991年 | 13篇 |
1990年 | 14篇 |
1989年 | 17篇 |
1988年 | 14篇 |
1987年 | 11篇 |
1986年 | 13篇 |
1985年 | 22篇 |
1984年 | 9篇 |
1983年 | 8篇 |
1982年 | 3篇 |
1981年 | 13篇 |
1980年 | 9篇 |
1979年 | 5篇 |
1978年 | 9篇 |
1977年 | 10篇 |
1976年 | 4篇 |
1969年 | 1篇 |
1957年 | 1篇 |
排序方式: 共有1160条查询结果,搜索用时 15 毫秒
1.
2.
Ulrich Weber Christian WA Pfirrmann Rudolf O Kissling Juerg Hodler Marco Zanetti 《BMC musculoskeletal disorders》2007,8(1):20
Background
Ankylosing spondylitis is a chronic inflammatory rheumatic disorder which usually begins in early adulthood. The diagnosis is often delayed by many years. MR imaging has become the preferred imaging method for detection of early inflammation of the axial skeleton in ankylosing spondylitis. 相似文献3.
4.
5.
6.
J. Labadie M. J. D. van Tol N. H. Dijkstra M. van der Kaaden C. M. Jol van der Zijde G. G. de Lange F. E. Zwaan J. M. Vossen 《British journal of haematology》1992,82(2):437-444
A rapid recovery of specific humoral immunity in the recipient of an allogeneic bone marrow transplantation (BMT) can be observed after immunization of the donor before graft sampling. This has been attributed to transfer of specific immunity from donor to recipient. However, to maintain the concept of transfer the origin of the antibody producing cells in the recipient after BMT must be demonstrated. To this end, donor-recipient pairs with differences in Gm-allotypes were selected and immunized before BMT with the neo-antigen Helix pomatia haemocyanin (HPH) according to three immunization protocols. Additionally, the recipients were immunized at day 42 after BMT. Serum samples were weekly obtained from the recipients in the first 100 d after BMT. The origin of HPH-specific antibody producing cells was assessed by two approaches: (1) determination of the Gm-allotypes of anti-HPH antibodies within a distinct IgG subclass, (2) analysis of anti-HPH antibody spectrotypes by isoelectric focusing combined with immunoblotting. The results obtained with these two approaches show concordance in most instances and led to the conclusion that the antibody producing cells are of donor origin. 相似文献
7.
8.
Role of Escherichia coli K capsular antigens during complement activation, C3 fixation, and opsonization. 总被引:2,自引:0,他引:2 下载免费PDF全文
W C Van Dijk H A Verbrugh M E van der Tol R Peters J Verhoef 《Infection and immunity》1979,25(2):603-609
Escherichia coli strains with K capsular polysaccharides are relatively resistant to phagocytosis by polymorphonuclear leukocytes, in contrast to E. coli strains without K antigens. This inhibition of phagocytosis is related to an impaired recognition of the K+ strains by the phagocytes due to ineffective opsonization. All five strains without K antigens were readily phagocytized after opsonization in 5% normal serum, compared with no uptake of the K+ strains. Evidence is presented that the decreased opsonization of the K+ strains in normal serum is caused by a low rate of complement activation of the strains, with subsequent absence of C3b fixation or C3d fixation or both to the cell wall of the bacteria. After removal of the K+ antigens by heating of a K+ E. coli strain, the strain was able to activate complement, to bind C3b or C3d or both, and to become opsonized. Complement was then activated via the classical and alternative pathways, which was comparable to the complement consumption by K- E. coli. 相似文献
9.
目的:研究低氧时小鼠肺组织中低氧诱导因子-1α(HIF-k)表达的变化。方法:实验用雄性小鼠,低氧仓浓度分别为10%、7%、5%。用免疫荧光组织化学技术及共聚焦显微术,检测小鼠在低氧条件下肺组织中HIF-1α表达的变化。结果:正常组小鼠肺组织HIF-1α无表达,低氧组HIF-1α表达增加,且随低氧时间的延长及低氧强度的增加而增强。结论:低氧可诱导小鼠肺组织中HIF-1α的表达增强,(HIF-k)可能参与肺组织细胞凋亡的发生。 相似文献
10.
Cristina Benito Eloína González-Mancebo M Dolores Alonso Díaz de Durana Rosa M Tolón Montserrat Fernández-Rivas 《Annals of allergy, asthma & immunology》2007,98(6):580-584
BACKGROUND: Coconut (Cocos nucifera) is a monocotyledonous plant of the Arecaceae family. Allergy to coconut is infrequent, with only 5 cases reported so far in the medical literature. OBJECTIVE: To identify coconut allergens in 2 patients allergic to this food. METHODS: We describe 2 patients allergic to coconut: an adult pollen-allergic patient monosensitized to coconut who presented with severe oropharyngeal symptoms and a child with a previous allergy to walnut, not allergic to pollen, who developed anaphylaxis on coconut ingestion. Both patients had positive skin prick test results and serum specific IgE (CAP) to coconut. IgE sodium dodecyl sulfate-polyacrylamide gel electrophoresis immunoblotting was performed to identify the allergens involved, and a strong IgE binding band detected in both patients was further analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS). Stability to pepsin digestion of the coconut extract and its cross-reactivity with tree nuts were studied. RESULTS: An immunoblot showed an almost identical profile of IgE binding proteins in the coconut extract in both patients who reacted strongly to a band of approximately 29 kDa. The peptide analysis by MALDI-TOF MS of this band obtained the sequence GHGKREDPEKR. The protein with the highest correlation with this peptide was found to be a 7S globulin from Elaeis guineensis, another oil palm species also belonging to the Arecaceae family. The 29-kDa band was digested by pepsin in less than 1 minute. Cross-reactivity among coconut, walnut, and hazelnut was demonstrated by CAP inhibition in patient 2. CONCLUSION: We have identified a 7S storage protein as a novel coconut allergen. 相似文献