宫颈癌的介入化疗与栓塞

目的：评价超选择介入化疗与栓塞在宫颈癌中的作用。方法：1997-2000年15例宫颈癌患者行介入治疗,12例于介入术1-4个疗程后再行肿瘤切除术。结果：（1）宫颈癌介入治疗有效率为80%：（2）介入术后宫颈癌切除术率为80%;（3）副作用主要为胃肠道反应和骨髓抑制反应。结论：超选择髂内动脉插管化疗与栓塞可使宫颈癌缩小,为手术治疗创造条件,是治疗宫颈癌的一种新的有效辅助疗法。     

2-氨基异黄酮化合物的合成

本文报道了一系列2-氨基-3′-胺甲基-4′-羟基-6-取代异黄酮化合物(Ⅱ)的合成。中间体2-甲氧基-5-取代苯甲酸甲酯(Ⅲ)系用相转移催化技术同时进行醚化和酯化得到。Claisen缩合产物用三溴化硼脱甲基同时环化成2-氨基异黄酮化合物(Ⅵ)。利用Mannich反应在4′-羟基的邻位引入胺甲基制得目的物。药理筛选结果显示Ⅱbb′对小鼠具有明显的耐缺氧作用。     

Inhibition of acid-induced apoptosis by targeting ASIC1a mRNA with short hairpin RNA

Aim： To study the role of acid-sensing ion channel （ASIC） 1a in the cell death and apoptosis induced by extracellular acid in C6 glioma cells. Methods： The stable ASIC1a-silenced C6 cell line, built with RNA interference technology, were confirmed by RT-PCR and Western blot analysis. The cell viability following acid exposure was analyzed with lactate dehydrogenase （LDH） and 3-（4,5- dimethylthiazol-2-y1）-2, 5-diphenyltetrazolium bromide （MTT） assay. The apoptotic cells dyed with Annexin-V and propidium iodide were measured with a flow cytometer, while the changes of cell cycle were also assayed. Results： The downregulation of ASIC la proteins by stable transfection of short hairpin RNA decreased the cell death percentage and increased cell viability following acid exposure with LDH and the MTT assay. The rate of apoptosis was lower in the ASIC 1a-silenced cell line than that in the wild-type C6 cell line. The percentage of sub-G0 cells was lower in the ASIC 1a-silenced C6 cells than that in the wild-type cells. Conclusion： Extracellular acid induced cell death and apoptosis via ASIC 1a mechanisms in the C6 glioma cells.     

Treatment Rate for Major Depressive Disorder in China: a Meta-Analysis of Epidemiological Studies

Psychiatric Quarterly - Major depressive disorder (MDD) is a common psychiatric disorder in China, but its reported treatment rate varies largely across different studies. The objective of this...     

复方麻芥透皮贴剂经皮肤给药的安全性研究

目的:考察复方麻芥透皮贴剂经皮肤给药安全性。方法:通过对健康家兔进行皮肤急性毒性试验、皮肤刺激性试验及健康豚鼠皮肤过敏性试验,观察复方麻芥透皮贴剂经皮肤用药的急性毒性、刺激性和过敏性。结果:复方麻芥透皮贴剂对破损皮肤组试药中、高剂量组表现为轻度刺激性,其余各组均均无急性毒性和刺激性。对豚鼠完整皮肤无致敏作用。结论:本实验所用复方麻芥透皮贴剂经皮肤给药安全。     

宫颈癌的介入化疗与栓塞

目的：评价超选择介入化疗与栓塞在宫颈癌中的作用。方法：1997～2000年15例宫颈癌患者行介入治疗,12例于介入术1～4个疗程后再行肿瘤切除术。结果：（1）宫颈癌介入治疗有效率为80%;（2）介入术后宫颈癌切除术率为80%;（3）副作用主要为胃肠道反应和骨髓抑制反应。结论：超     

酸感受离子通道亚基1a敲减后的细胞抗酸性研究

目的：通过酸感受离子通道（ASIC）1a表达抑制的细胞模型，探讨在酸性条件下ASIC1a对细胞生长增殖的影响。方法：以大鼠神经胶质瘤（C6）为模型细胞，利用RNAi技术建立ASIC1a表达抑制的细胞模型。通过绘制细胞生长曲线、集落形成试验、MTT试验和乳酸脱氢酶（LDH）释放试验来观察不同pH条件下，ASIC1a表达抑制细胞和阴性对照细胞生长增殖能力的变化。结果：RNAi技术可明显抑制C6细胞ASIC1a蛋白的表达。在正常pH条件下，ASIC1a表达缺失对C6细胞的生长增殖没有显著影响。但在不同酸性条件下，ASIC1a表达抑制细胞与阴性对照细胞相比对酸的耐受性明显提高，表现为细胞损伤减轻，存活数目明显增多。结论：C6细胞中有ASIC1a，ASIC1b，ASIC2a，ASIC2b和ASIC3亚基的表达。抑制ASIC1a的表达不影响正常pH条件下细胞的生长增殖，但能明显提高细胞对酸的耐受性，提示ASIC1a可能是潜在的抗酸靶分子。     

Ent-kauranoids from Isodon rubescens var. taihangensis

Two new compounds, rubescensins Q and R (1 and 2), and a new acetonide derivative (3) of lasiodonin, together with thirteen known analogues, oridonin (4), ponicidin (5), wikstroemioidin B (6), lasiodonin (7), lasiokaurin (8), enmenol (9), 1-O-beta-D-glucopyranosyl-enmenol (10), trichokaurin (11), the acetonide of maoyecrystal F (12), rabdoternins A-D (13-16), have been isolated from Isodon rubescens var. taihangensis. The structures of the new compounds were elucidated on the basis of spectroscopic methods, especially the 2D NMR spectral analysis. Compound 3 exhibited cytotoxicity against K562, Bcap37, CA, CNE, BIU87, BGC823, and HeLa cell lines.     

诺如病毒快速检测试剂的评价

目的评价德国R—Biopharm公司生产的RIDA QUICK诺如病毒快速检测试剂卡（免疫层析法）的准确性、安全性以及与对照试剂的等效性。方法以上市的IDEA Norovirus检测试剂盒（酶联免疫法）平行测定结果为参考,评价RIDA QUICK诺如病毒快速检测试剂卡（免疫层析法）的灵敏度、特异性及符合率。结果临床检测样本1042份,新型快速检测试剂诺如病毒检测特异性98．4％,灵敏度92．4％,与对照试剂的符合率为97．6％。结论RIDA QUICK诺如病毒快速检测试剂卡对于诺如病毒抗原的检测具有良好的特异性和灵敏度。     

聚乙烯醇加明胶海绵栓塞支气管动脉治疗大咯血46例临床分析

OBJECTIVE: To evaluate the effect of bronchial artery embolization (BAE) with polyvinyl alcohol (PVA) foam and gelatinum sponge (GS) for serious hemoptysis. METHOD: Of 46 patients with severe hemoptysis, BAE with only GS was performed in 21 cases and with both PVA and GS in 25 cases. RESULT: The total efficacy rate of BAE was 91.3%; and comparable between PVA+GS and GS groups (92.0%; vs 90.5%;, P>0.05). The total recurrence rate after BAE was 26.2%;, but lower in PVA+GS group than in GS group (11.3%; vs 42.1%;, P<0.05). No serious complications occurred in these patients after BAE. CONCLUSION: BAE is effective and safe for management of serious hemoptysis, and treatment with PVA+GS may effectively lower the recurrence rate.