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排序方式: 共有474条查询结果,搜索用时 38 毫秒
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目的:观察非程控降温、-80℃冻存的方法对自体外周血十细胞(APBSC)的保存效果。方法:以6%羟乙基淀粉(HES)、5%二甲基亚砜(DMSO)及4%人血白蛋白(ALB)的混合物为冷冻防护剂,将APBSC直接置于-80℃下保存,冻存前反复苏后测定APBSC的CFU-GM、BFU-E;观察移植后造血功能重建情况。结果:13例患者白细胞在十3~+7天下降至(0.0~0.1)×10/L,白细胞(0.0~0.2)×109/L持续时间3~6天,于+9~+11天恢复至1.0X109/L以上.中性粒细胞绝对值(ANC)于+9~+11天达到0.5X109/L。血小板在+3~+7天下降至(2.0~21)×109/L,于+8~+15天恢复至20×109/L以上。CFU-GM、BFU-E回大率分别为76.5%、78.4%。结论:非程控降温、-80℃冻存是一简便、经济、有效的自体外周血于细胞保存方法。 相似文献
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MU Orji TI Mbata OU Kalu 《Malawi medical journal : the journal of Medical Association of Malawi》2005,17(4):128-130
A survey of bacteria contamination of hospital staff apparel in use in Anambra State, Nigeria, was carried out to determine the extent of contamination by clinically important bacteria. Of a total of 125 swab samples of hospital staff apparel, 72 (58%) showed bacterial contamination including 32 (70%) of 46 samples from hand gloves, 28 of 45 (62%) samples from protective gowns, and 12 of 34 (35%) samples from face-shields. The potentially pathogenic bacteria isolated were Salmonella spp, Proteus vulgaris, Shigella dysenteriae, Pseudomonas aeruginosa and Staphylococcus aureus. The isolation of clinically important bacteria from the apparel suggests the need for improved infection control measures. 相似文献
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目的:研究大鼠急性肺栓塞模型肺组织中衰老标记蛋白质30(SMP-30)的表达变化及其对Fas诱导的细胞凋亡的影响。方法:建立大鼠急性肺栓塞模型,分别在急性肺栓塞后1、8、24和48 h进行支气管肺泡灌洗,然后开胸取出肺组织。常规提取肺组织的总RNA和总蛋白,以正常组为对照,采取半定量RT-PCR的方法研究SMP-30在mRNA水平表达的变化;采用Western blotting方法进一步验证SMP-30在蛋白水平表达的变化;采用免疫组织化学方法检测大鼠肺组织中SMP-30以及肺泡巨噬细胞中IL-8在肺栓塞前后表达的变化及其组织分布情况;采用TUNEL法研究急性肺栓塞后组织细胞的凋亡情况;最后采用ELISA法检测急性肺栓塞后肺泡灌洗液中sFasL的浓度变化。结果:在大鼠急性肺栓塞后的不同时点,SMP-30的mRNA水平和蛋白水平均逐渐降低,在24和48 h下降最为明显。免疫组化研究表明SMP-30主要分布在支气管黏膜上皮细胞和肺泡上皮细胞,急性肺栓塞后SMP-30在上述细胞内的表达均明显降低。TUNEL染色发现随着SMP-30表达的降低,肺组织内出现明显的细胞凋亡现象,同时肺泡灌洗液中sFasL的浓度升高,肺泡巨噬细胞内IL-8的表达也明显升高。结论:大鼠急性肺栓塞后肺组织内SMP-30的表达明显降低,可能促进Fas-FasL细胞凋亡系统的活化。 相似文献
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Baumgartner FJ Omari BO Robertson JM Nelson RJ Pandya A Pandya A Milliken JC 《The Annals of thoracic surgery》2000,70(2):442-447
BACKGROUND: The aim of this study was to determine patterns of anatomic, clinical, and operative features in surgical endocarditis (SE) with annular abscess (AA). METHODS: The study consisted of a retrospective analysis of SE cases with AA between 1981 and 1997. RESULTS: A total of 41 cases with AA were found in 106 consecutive SE cases. There was a higher incidence of AA in aortic (37 of 71 [52%]) (p<0.01) compared to mitral (6 of 42 [14.3%]) or tricuspid (0 of 12) infections. However, the mitral abscesses had a greater tendency toward fistula or pseudoaneurysm formation (4 of 6 [67%]) than other valve abscess cavities (7 of 46 [15%]) (p<0.01). Severe heart failure (p<0.01), heart block (p<0.05), and fistula/pseudoaneurysm (p<0.001), were more often found in SE with AA than without. There were 46 separate aortic AA in 37 instances of aortic valve SE. Of these, 31 of 46 (67%) were less than 1 cm (group 1), 10 of 46 (22%) were large but confined to a given cusp annulus (group 2), 4 of 46 (8.6%) were large between multiple cusps (group 3), and 1 of 46 (2.2%) was circumferential (group 4). There were four instances of aortoventricular discontinuity. Group 1 abscesses were repaired by local closure without a patch significantly more often than the other groups. The mortality of SE with AA was significantly greater for larger AA (groups 3 and 4, 3 of 5 [60%]) than for smaller AA (groups 1 and 2, 0 of 36) (p<0.001). There were six separate mitral AA in six instances of mitral SE, five requiring patch repair. The 30-day operative mortality for AA cases was 3 of 41 (7.3%) compared to 2 of 65 (3.1%) without AA. All AA mortalities involved large AA in the aortic valve position. Of 35 mechanical valves placed for AA, only one required subsequent removal for prosthetic endocarditis. CONCLUSIONS: Annular abscesses are most frequent in aortic AA, but fistulas/pseudoaneurysms are more frequent in mitral AA. Small to moderate aortic AA can be managed by local closure without an increased mortality compared to SE without AA. Patients with large aortic AA have a higher operative mortality. Mechanical prostheses are safe and effective for the majority of patients with AA. 相似文献
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目的采用活性指导分离的方法研究百合科藜芦属植物Veratrum virid和藜芦V. nigrum干燥根及根茎的抗真菌活性成分。方法利用多种色谱技术进行分离纯化。通过现代波谱技术对单体化合物进行结构鉴定。结果从V.virid的甲醇提取物中分离得到10个化合物,分别鉴定为rubivirine-3-O-β-glucopyranoside(1)、veratrosine(2)、veratramine(3)、pseudojervine(4)、rubivirine(5)、resveratrol 3,4′-O,O′-di-β-D-glucopyranoside(6)、mulberroside A(7)、resveratrol 4′-O-β-D-glucopyranoside(8)、resveratrol 3-O-β-D-glucopyranoside(9)、oxyresveratrol-2-O-β-D-glucopyranoside(10);从藜芦的甲醇提取物中分离得到9个化合物,分别鉴定为veratrosine(2)、veratramine(3)、pseudojervine(4)、rubivirine(5)、verussurinine(11)、veralosidine(12)、jervine(13)、γ-chaconine(14)、germidine(15)。结论其中化合物1~5、11~15为甾体生物碱类化合物,6~10为二苯乙烯苷类化合物。化合物1为新的甾体生物碱类化合物;化合物5~10、14为首次从该属植物中分离得到。采用纸片扩散法评价化合物的抑真菌活性,结果显示化合物14对新生隐球菌和克柔念珠菌都具有较好的抑制活性,半数抑制浓度(IC50)分别为4.1、19.8μg/mL。并且化合物14在100μmol/L浓度下对4株人肿瘤细胞都不具有抑制活性。 相似文献
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Vitamin D deficiency and level of asthma control in women from North of Jordan: a case–control study
Shaher Samrah Ibrahim Khatib Muntaser Omari Basheer Khassawneh Suleiman Momany Ammar Daoud 《The Journal of asthma》2014,51(8):832-838
Introduction: Reduced vitamin-D levels in patients with asthma have been associated with impaired lung function, increased airway hyper-responsiveness, and reduced glucocorticoid responsiveness. Nationwide studies revealed a considerable prevalence of vitamin-D deficiency (VDD) in Jordanian women. Objective: A case–control study was conducted to determine the relationship between serum vitamin A and D levels and asthma among women in North of Jordan. Methods: Sixty-eight asthmatics, age range between 14 and 65 years and 77 healthy women, age range between 19 and 51 years, were enrolled. Asthma severity was classified using Global Initiative for Asthma (GINA) guidelines and Asthma Control Test (ACT) questionnaire. Serum vitamin-A and 25-hydroxyvitamin-D (25(OH)D3) levels were measured using high-performance liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods, respectively. Results: The prevalence of VDD (<15?ng/ml) was higher but not statistically significant for women with asthma compared with controls (95.6% vs. 87.0%; p?=?0.070). The severity of VDD correlated with the number of asthma medications (p?=?0.020). 25(OH)-D3 serum levels directly correlated with asthma control level using ACT score (p?=?0.012) and GINA classification (p?=?0.046). After adjusting for age, the odds of having VDD for asthmatic women were 35.9 times higher than that for women with no asthma. There was no difference in serum vitamin-A level between healthy and asthmatic women (p?=?0.214) and none had vitamin-A deficiency (<200?µg/dl). Conclusions: VDD is prevalent in women with asthma in northern Jordan. The severity of VDD correlated with poor asthma control and a need for more medications to control asthma. There was no association between vitamin-A and asthma. 相似文献