Comparative morphological study on the lingual papillae and their connective tissue cores (CTC) in reeves’ muntjac deer (Muntiacus reevesi)

The lingual papillae and their connective tissue cores (CTC) from Reeves’ muntjac deers (herbivorous artiodactyla) were studied using light and scanning electron microscopy and then compared to those of other mammalian species. At the posterior portion of the tongue, the Reeves’ muntjac has a lingual prominence on which large conical papillae are distributed. On the dorsal surface of the anterior tongue, numerous filiform papillae were found. Externally, each filiform papilla consists of a rod-shaped main process and several small accessory processes. Their CTCs consist of 10 or more rod-shaped processes arranged in a horseshoe pattern and several posterior processes forming a small circular pattern. This structure is a common characteristic of artiodactyla, through which Reeves’ muntjac deer can be categorized in a position in the artiodactyla class lying between the bighorn sheep and the East African bongo. Fungiform papillae are distributed among the filiform papillae on the anterior portion of the tongue. Large fungiform papillae are also sparsely distributed on the lingual prominence and have several taste buds in the epithelium on the surface. Ten or more vallate papillae are distributed at the postero-lateral area of the lingual prominence and numerous taste buds are distributed in the epithelium of their side.     

Hemodynamic changes between different anatomically designed stents after carotid stenting: a prospective multicenter study

Journal of Neurology - The aim of this study was to investigate differences in the dynamic changes and risk factors of hemodynamic depression (HD) between straight and tapered carotid stenting (SCS...     

人KIAA1173基因的克隆、转染及生物学特性的实验研究

目的建立表达KIAA1173基因的鼻咽癌6-10B细胞模型,并观察转染细胞的生物学活性.方法从新鲜肌肉组织中提取总RNA,采取逆转录PCR得到人KIAA1173基因cDNA.获得的人KIAA1173基因克隆到真核表达载体pcDNA3.1（＋）,进行酶切和序列鉴定.将重组质粒pcDNA3.1（＋）-KIAA1173和空载体利用阳离子脂质体介导转入鼻咽癌6-10B细胞,经G418筛选阳性克隆.用RT-PCR、原位杂交及免疫细胞化学等方法检测KIAA1173基因在6-10B细胞中的稳定表达情况,并通过MTT法、肿瘤细胞侵袭实验及裸鼠体内成瘤性实验方法,检测KIAA1173基因对鼻咽癌细胞的增殖、侵袭及成瘤能力的影响.以空白质粒转染组作为对照.结果在mRNA和蛋白水平证实,转染细胞内有KIAA1173基因的高表达,表明细胞可表达人KIAA1173基因.6-10B在转染了pcDNA3.1（＋）-KIAA1173后,较转染空载体pcDNA3.1（＋）,肿瘤细胞的增殖能力、侵袭能力及裸鼠体内成瘤能力明显降低（P＜0.05）.结论结果提示KIAA1173基因可能是一鼻咽癌肿瘤抑制基因.获得生物学活性稳定的KIAA1173基因高表达的鼻咽癌细胞模型,为进一步研究奠定了基础.     

细胞毒性T淋巴细胞相关抗原4基因型与中国南方人群Graves’病的关系

【目的】探讨细胞毒性T淋巴细胞相关抗原-4（cytotoxic T lymphocyte associated antigen4）5因第一外显子A／G（49）多态性与中国南方汉族人群Graves’病的关系。【方法】用PCR—RFLP确定CTLA-4基因第1外显子49位点基因型，分析广东地区汉族人Graves’病患者（GD组）120例及123名正常人（对照组）CTLA-4基因多态性。并把GD患者按性别、家族史、TRAb、突眼等分别分两亚组后分析CTLA-4基因第1外显子A49G位点基因型及等位基因的频率。【结果】CTLA-4基因第1外显子49位点G等位基因频率在GD组为0．704，对照组为0．679，A等位基因频率在GD组为0．296，对照组为0．321，基因型频率GG在GD组为0．483，对照组为0．447，AA在GD组为0．089，对照组为0．075，二组间均无统计学差异，P值均〉0．05。在GD组，按性别、家族史、TRAb及突眼进行分亚组后分析，CTLA-4基因A49G位点的基因型、等位基因频率也没有表现出差异俨值均〉0．05）。【结论】CTLA-4基因第一外显子A/G（49）多态性可能不是中国南方汉族人Graves’病的主要易感因素。     

射频消融治疗肥厚性鼻炎的疗效分析

目的 探讨慢性肥厚性鼻炎经低温射频消融治疗的临床疗效。方法 选择66例慢性肥厚性鼻炎患者,经药物治疗无效,术前经鼻窦CT检查排除副鼻窦炎、鼻中隔偏曲,取用双侧下鼻甲粘膜下进行低温射频消融治疗1至2次,术后伤口无需特殊处理,口服抗生素5～7天。结果 术后随诊3个月以上。痊愈42例(占63.63%),好转18例(占27.27%),无效6例(0.9%),总有效率为90.9%,所有病例未出现伤口大出血、鼻腔粘膜撕裂、坏死等并发症,患者术中可出现轻度的疼痛,但尚能忍受。结论 低温射频消融术是治疗慢性肥厚性鼻炎的一种有效、安全、微创,而且可在基层医院广泛开展的治疗方法。     

努力提升科技论文的科学性

作者提交的科技文稿中存在各种各样的学术性错误,科技期刊编辑有责任帮助作者发现并改正这些错误,努力提升科技论文的科学性。科技论文中常见的学术性错误有：逻辑错误;公式错误;计算错误;数据错误等。树立强烈的责任感,应用科学的分析方法,建立合理的编校制度,是减少乃至消除论文中学术性错误的有效措施。     

Association of the <Emphasis Type="Italic">COQ2</Emphasis> V393A variant with risk of multiple system atrophy in East Asians: a case–control study and meta-analysis of the literature

Recent studies in Japan have associated multiple system atrophy (MSA), a neurodegenerative disease of uncertain etiology, with polymorphism in the COQ2 gene. This led us to explore whether the same polymorphism is associated with MSA in Han Chinese and more broadly in East Asians. We conducted a case–control study with 82 Han Chinese with probable MSA and 484 gender- and age-matched healthy subjects, genotyping them using the ligase detection reaction. The results were meta-analyzed together with data from four previous studies to gain a broader picture of possible disease associations in East Asian populations. The COQ2 variants M78V and R337X were not detected in our Han Chinese patients or controls; only the heterozygous V393A variant (CT genotype) was detected. The frequency of this genotype was significantly higher in patients (7.3 %) than in controls (1.86 %; OR 4.17, 95 % CI 1.44–12.04, p = 0.004). Subgroup analysis among patients showed a significant association of V393A with MSA involving cerebellar signs (MSA-C; OR 4.59, 95 % CI 1.36–15.48, p = 0.007), but not with MSA involving parkinsonism (MSA-P). Meta-analysis of our results in Han Chinese with data from case–control studies in Japan, Korea, mainland China and Taiwan showed a significant association of V393A with MSA (OR 2.05, 95 % CI 1.29–3.25, p = 0.002), which subgroup analysis showed to be significant for MSA-C (OR 2.75, 95 % CI 1.98–3.84, p < 0.001) but not for MSA-P (OR 1.25, 95 % CI 0.64–2.46, p = 0.51). These findings provide evidence that the previously reported association of COQ2 V393A polymorphism with increased risk of MSA in Japanese also applies to Han Chinese, as well as more broadly to other East Asian populations. This association may be particularly strong for MSA-C.     

脂肪乳剂的临床应用进展

脂肪乳剂是肠外营养液中非蛋白能源之一,不仅能提供热能和必需脂肪酸,还能维持细胞结构和功能,影响机体免疫功能,有助于疾病康复。不同脂肪乳剂不同的代谢特点决定了其在临床的不同应用。本文对脂肪乳剂的临床应用进展作一综述。     

Complete genome sequence of Escherichia coli O157:H7 lytic phage JL1

A novel virulent phage named JL1 against Escherichia coli O157:H7 was isolated from raw sewage. It was found that JL1 has an icosahedral head and a long flexible non-contractile tail. The complete genome of JL1 is composed of a linear double-stranded DNA of 43,457 base pairs in length, with 54.77 % G+C content and 60 putative open reading frames. Morphology and bioinformatics analysis revealed that phage JL1 is a member of the family Siphoviridae of the order Caudovirales. It is different from previously reported phages of E. coli O157:H7 but is homologous to Sodalis phage SO-1, Shigella phage EP23, Escherichia phage HK578 and Escherichia phage SSL-2009a.     

早产儿机械通气125例临床分析

目的探讨早产儿机械通气治疗策略及并发症,提高危重早产儿生存率。方法将63例接受通气的早产儿作为治疗组,选择同期接受通气的足月儿62例作为对照组,比较两组患儿机械通气的并发症和接受机械通气治疗的主要原发疾病。结果对照组的并发症发生率37．1％远低于治疗组的并发症发生率52．4％,差异有统计学意义（P〈0．05）,治疗组主要原发疾病是HMD、频繁呼吸骤停和肺出血,对照组的主要原发疾病是吸人性肺炎、颅内出血,结果具有统计学意义（P〈0．05）。结论呼吸机相关性肺炎,胃肠功能障碍,心功能抑制,支气管肺发育不良是最常见并发症,在维护重要脏器功能的同时,要积极治疗原发病。