E-cadherin和α-catenin及β-catenin在乳腺癌组织中的表达及意义

目的：探讨上皮性钙黏素（E-cd）及α-连接素（cat）、β-cat在浸润性乳腺癌中的表达及意义。方法：免疫组化SP法。结果：60例乳腺癌组织中E-cd、α-cat、β-cat异常表达率分别为51．7％、61．7％和70％，E-cd的表达与乳腺癌分化程度、临床TNM分期、淋巴结转移显著相关，P〈0．005～0．0005，α-cat、β-cat的表达与分化程度、淋巴结转移无关。如果将E-cd、α-cat和β-cat作为一类蛋白分析，这类蛋白分子的异常表达与淋巴结转移显著相关，P〈0．05。结论：E-cd表达下调在乳腺癌浸润、转移中有重要作用，E-cd单独表达下调足以破坏E-cd／cat复合物的功能，导致癌细胞浸润转移。     

米非司酮致子宫内膜简单型增生二例临床分析

近年来，随着米非司酮应用的日趋广泛，其并发症亦不断增加，尤其是一些少见的以及一些始料未及的并发症，更应引起注意。我院2004年门诊诊治的二例病例，1例为子宫肌瘤剜除术后，1例为子宫腺肌症，在连续口服米非司酮4～6个月后，出现不规则的阴道多量流血，后经诊断性刮宫，二例病理检查均提示为子宫内膜简单型增生。现报道如下：     

Hemodynamic changes between different anatomically designed stents after carotid stenting: a prospective multicenter study

Journal of Neurology - The aim of this study was to investigate differences in the dynamic changes and risk factors of hemodynamic depression (HD) between straight and tapered carotid stenting (SCS...     

纵隔镜在上腔静脉阻塞综合征诊断中的应用

目的探讨纵隔镜在上腔静脉阻塞综合征（SVCOS）病因诊断中应用的可行性和价值。方法12例上腔静脉阻塞综合征病人，男9例，女3例；年龄16，71岁，平均45．2岁。行颈部纵隔镜检（CM）7例，胸骨旁纵隔镜检（PSM）5例。结果11例获得病理学诊断，阳性率91．7％。其中原发肺癌8例，淋巴瘤3例，未能明确诊断1例；所有病例手术经过均顺利，无并发症和死亡；1例淋巴瘤术后症状加重，化疗后缓解。结论纵隔镜检查对于SVCOS病例同样安全可靠，可作为其他方法不能确诊病例的可靠检查方法。     

Comparative proteomic profiling of murine skin

Mammalian skin is regularly exposed to different environmental stresses, each of which results in specific compensatory changes in protein expression that can be assessed by proteomic analysis. We have established a reference proteome map of BALB/c murine skin allowing the resolution of greater than 500 protein spots in a single two-dimensional polyacrylamide gel. Forty-four protein spots, corresponding to 28 different cutaneous proteins, were identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and the Mascot online database searching algorithm. Twenty-five proteins were expressed at higher levels in the epidermis, whereas only nine were found predominantly in the subepidermal tissues. A subset of protein spots exhibited strain-specific expression. Proteins of diverse function were identified, including those involved in stress response, apoptosis, growth inhibition, the maintenance of structural integrity, translational control, energy metabolism, calcium binding, cholesterol transport, and the scavenging of free radicals. Prohibitin expression was detected cutaneously, with more abundant protein and mRNA levels in the epidermis. Five molecular chaperones including protein di-sulfide isomerase, 78 kDa glucose-regulated protein precursor, heat shock protein 60 (HSP60), HSP70, and HSP27 were also identified. Of these, HSP27 expression was confined mainly to the epidermis, and expression of protein disulfide isomerase was found primarily in the subepidermal tissues. Proteomic analysis of skin following heat or cold shock resulted in increased levels of HSP27, HSP60, and HSP70 suggesting involvement of these chaperones in the cutaneous response mechanism to temperature stress. These data establish numerous reference markers within the proteome map of murine skin and provide an important framework for future efforts aimed at characterization of the epidermal and subepidermal responses to environmental changes.     

慢性心力衰竭患者血清IL-1β、TNF-α水平与左室平均室壁应力测定

目的：探讨慢性心力衰竭（CHF）患者血清白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平与左室平均室壁应力的关系及临床意义。方法：CHF患者62例，另设15例健康人作对照。血清TNF-α、IL-1β含量测定采用ELISA法。心脏超声测定左室舒张末期和收缩末期内径（Dd和sd）、室间隔舒张末期和收缩末期内径（IVSd和IVSs），左室后壁舒张末期和收缩末期厚度（PWd和PWs），计算左室平均室壁应力（MWS）。结果：CHF患者血清IL-1β、TNF-α含量及MWS高于对照组；CHF患者血清IL-1β、TNF-α含量与MWS呈正相关（r分别为0．489，0．458，P均〈0．001）。结论：CHF患者血清IL-1β、TNF-α含量升高，室壁应力增加，它们可能相互作用，共同参CHF的致病机制。     

Myc-driven murine prostate cancer shares molecular features with human prostate tumors

Increased Myc gene copy number is observed in human prostate cancer. To define Myc's functional role, we generated transgenic mice expressing human c-Myc in the mouse prostate. All mice developed murine prostatic intraepithelial neoplasia followed by invasive adenocarcinoma. Microarray-based expression profiling identified a Myc prostate cancer expression signature, which included the putative human tumor suppressor NXK3.1. Human prostate tumor databases revealed modules of human genes that varied in concert with the Myc prostate cancer signature. This module includes the Pim-1 kinase, a gene known to cooperate with Myc in tumorigenesis, and defines a subset of human, "Myc-like" human cancers. This approach illustrates how genomic technologies can be applied to mouse cancer models to guide evaluation of human tumor databases.     

不同岗位不同军龄士兵应激状态下的心理状况分析

目的：探讨在抗击传染性非典型肺炎的应激状态下，参战一线士兵心理卫生状况，并分析不同年龄及不同岗位士兵间症状因子评分的差异特征。方法：于2003—05／06选择北京解放军小汤山医院男性战士141人（包括机关士兵）为观察对象进行调查。采用症状自评量表对其心理状态进行调查。该量表共有90个项目并分为躯体化、强迫症状、人际关系、抑郁、焦虑、敌对、恐怖、偏执狂、精神病性等9个症状因子分和总症状指数．采用1-5级评分。评估标准：单项因子分及总症状指数达到3分以上定为阳性，提示可能存在明显的心理问题。在测试前采用统一的指导语，让士兵根据自己的实际情况做出真实的自我评定，独立完成。心理检测由经培训的专业人员进行。并与国内青年常模进行比较。结果：所有战士均完成测试，全部进人结果分析。①参战士兵症状自评量表阳性症状人数分布：参战士兵症状自评量表阳性症状人数依次为躯体化1人（0．7％）、强迫症状13人（9．2％）、人际关系敏感10人（7．1％）、抑郁7人（5．0％）、焦虑3人（2．1％）、敌对5人（3．6％）、恐怖9人（6．4％）、偏执10人（7．1％）、精神病性5人（3．6％）。其中有一项以上分数达到3分者共30人，占调查人数的21．6％。②参战士兵症状自评量表平均分最高的因子为强迫症状，其次为人际关系敏感，最低的因子则是精神病性，中间由高到低依次是敌对、偏执、忧郁、焦虑、躯体化和恐惧。除人际关系敏感因子外，参战士兵症状自评量表各因子分均高于国内青年组常模，差异有显著性。③机关兵的总症状指数和各因子分与非机关兵比较，前者的人际关系敏感因子分显著高于后者。④第2年军龄的战士总症状指数及人际关系、抑郁、恐惧、偏执等因子分显著高于第4年军龄的战士。第2年军龄的战士恐怖因子分也明显高于第1年军龄者（t=2．37，P〈0．05）。结论：①参战一线士兵症状自评量表的总分、总均分、阳性项目数、躯体化症状、人际关系敏感、抑郁、焦虑、敌对、恐怖、偏执狂、精神病性等标准分显著高于常模组，具有较高的心理异常发生率。②不同岗位，不同年龄士兵的心理健康评分有差异。