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排序方式: 共有327条查询结果,搜索用时 31 毫秒
1.
F. Aragona P. Ferrarese G. Passerini Glazel 《International urology and nephrology》1990,22(5):493-497
The effects of monopolar and bipolar diathermy systems were studied in 12 male Sprague-Dawley rats. Fulguration was achieved by application of a standard current (13 W for 1 s) on the foreskin. Tissue damage was assessed by light microscopy. Results suggest that bipolar probe causes less tissue damage than its monopolar counterpart. It has proved to be of value in plastic and paediatric procedures and is recommended for the urologist's armamentarium. 相似文献
2.
Agostoni E. Santoro P. Frigerio R. Frigo M. Beghi E. Ferrarese C. 《Neurological sciences》2004,25(3):s187-s189
Neurological Sciences - Headache is one of the most common symptoms that leads patients to the emergency room (ER) and is often related to diseases requiring prompt diagnosis and immediate... 相似文献
3.
Sala G Galimberti G Canevari C Raggi ME Isella V Facheris M Appollonio I Ferrarese C 《Neurobiology of aging》2003,24(7):909-914
Various studies suggested that inflammation is involved in the pathogenesis of Alzheimer's disease (AD). We investigated cytokine release from LPS-stimulated blood cells of 32 AD patients, with different disease severity, compared to 16 age-related controls. A significant decrease of IL-1beta and IL-6 secretion was observed in severely demented patients; TNF-alpha release was also decreased, but not significantly. By contrast, mild and moderate patients showed a cytokine release similar to controls. IL-1beta, IL-6 and TNF-alpha secretion was negatively correlated with the severity of dementia, quantified by the MMSE. Our data suggest that alterations of the immune profile are associated with AD progression. 相似文献
4.
Ferrarese C Zoia C Pecora N Piolti R Frigo M Bianchi G Sala G Begni B Riva R Frattola L 《Journal of neural transmission (Vienna, Austria : 1996)》1999,106(7-8):685-692
Summary. Defects in mitochondrial enzymes have been found not only in substantia nigra, but also in platelets from Parkinson's Disease
(PD) patients, suggesting a systemic impairment of energy metabolism. Since platelets present an energy-dependent glutamate
uptake similar to that described in central nervous system, glutamate uptake was determined in platelets from 34 PD patients
and 21 age-related normal controls, as Na+-dependent [3H]glutamate influx; glutamate level was also analyzed by reverse-phase HPLC. A 50% reduction of glutamate uptake (p < 0.001)
was observed in idiopathic PD patients, respect to controls and secondary parkinsonian syndromes. The decrease correlated
with the severity of PD, measured by the UPDRS (r = −0.54; P < 0.05). Glutamate level was increased in platelets of PD patients,
but was not correlated to the uptake decrease. Both phoenomena may be explained by the modifications of mitochondrial enzymes
described in platelets, which could be used as a peripheral model of glutamatergic function in PD.
Received October 7, 1998; accepted January 7, 1999 相似文献
5.
6.
The chaperone‐like sodium phenylbutyrate improves factor IX intracellular trafficking and activity impaired by the frequent p.R294Q mutation
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S. Pignani A. Todaro M. Ferrarese S. Marchi S. Lombardi D. Balestra P. Pinton F. Bernardi M. Pinotti A. Branchini 《Journal of thrombosis and haemostasis》2018,16(10):2035-2043
Essentials
- Missense mutations often impair protein folding, and thus intracellular trafficking and secretion.
- Cellular models of severe type I hemophilia B were challenged with chaperone‐like compounds.
- Sodium phenylbutyrate improved intracellular trafficking and secretion of the frequent p.R294Q.
- The increased coagulant activity levels (~3%) of p.R294Q would ameliorate the bleeding phenotype.
Summary
Background
Missense mutations often impair protein folding and intracellular processing, which can be improved by small compounds with chaperone‐like activity. However, little has been done in coagulopathies, where even modest increases of functional levels could have therapeutic implications.Objectives
To rescue the expression of factor IX (FIX) variants affected by missense mutations associated with type I hemophilia B (HB) through chaperone‐like compounds.Methods
Expression studies of recombinant (r)FIX variants and evaluation of secreted levels (ELISA), intracellular trafficking (immunofluorescence) and activity (coagulant assays) before and after treatment of cells with chaperone‐like compounds.Results
As a model we chose the most frequent HB mutation (p.R294Q, ~100 patients), compared with other recurrent mutations associated with severe/moderate type I HB. Immunofluorescence studies revealed retention of rFIX variants in the endoplasmic reticulum and negligible localization in the Golgi, thus indicating impaired intracellular trafficking. Consistently, and in agreement with coagulation phenotypes in patients, all missense mutations resulted in impaired secretion (< 1% wild‐type rFIX). Sodium phenylbutyrate (NaPBA) quantitatively improved trafficking to the Golgi and dose dependently promoted secretion (from 0.3 ± 0.1% to 1.5 ± 0.3%) only of the rFIX‐294Q variant. Noticeably, this variant displayed a specific coagulant activity that was higher (~2.0 fold) than that of wild‐type rFIX in all treatment conditions. Importantly, coagulant activity was concurrently increased to levels (3.0 ± 0.9%) that, if achieved in patients, would ameliorate the bleeding phenotype.Conclusions
Altogether, our data detail molecular mechanisms underlying type I HB and candidate NaPBA as affordable ‘personalized’ therapeutics for patients affected by the highly frequent p.R294Q mutation, and with reduced access to substitutive therapy.7.
Foderaro Giuseppe Isella Valeria Mazzone Andrea Biglia Elena Di Gangi Marco Pasotti Fabrizio Sansotera Flavia Grobberio Monica Raimondi Vanessa Mapelli Cristina Ferri Francesca Impagnatiello Valentina Ferrarese Carlo Appollonio Ildebrando Marco 《Neurological sciences》2022,43(5):3053-3063
Neurological Sciences - Mini-Mental State Examination (MMSE) is one of the most used tests for the screening of global cognition in patients with neurological and medical disorders. Norms for the... 相似文献
8.
Lucio Tremolizzo Emanuela Susani Christian Lunetta Massimo Corbo Carlo Ferrarese Ildebrando Appollonio 《Journal of neurology》2014,261(6):1196-1202
Identifying frontal impairment in ALS is an important goal albeit disease-dedicated tools are still scarce. For this reason, we decided to consider primitive reflexes (PRs), variably regarded as correlates of frontal release and/or of upper motor neuron (UMN) impairment, often in the setting of dementias. Specifically, the aims of this work consisted in assessing the exact prevalence of the combination of seven PRs in ALS, trying to clarify their role as putative proxies of cognitive impairment or of UMN dysfunction. In this cross-sectional study, 50 consecutive ALS outpatients were evaluated for the presence of: palmomental (PM), corneomandibular (CM), glabella tap (MY), rooting, sucking, snout, and grasping reflexes. Cognitive screening was performed by the Frontal Assessment Battery (FAB) and the Weigl’s Sorting test (WST); UMN dysfunction was concomitantly evaluated. PM, CM and MY were more frequently detected (62, 52, and 44 % of the ALS sample, respectively), while the other reflexes were under-represented. Patients displaying three or more PRs had significantly lower FAB and WST scores. On the other hand, UMN dysfunction was only moderately associated to PRs. In conclusion, PRs’ assessment is a promising complementary tool for screening cognitive impairment in ALS; however, further work will be necessary to establish its added value with respect to already existing ALS-dedicated screening tools for cognition. 相似文献
9.
Francesca D'Arcangelo Alberto Zanetto Camillo Aliberti Sarah Shalaby Monica Pellone Salvatore Stefano Sciarrone Chiara Becchetti Alberto Ferrarese Martina Gambato Francesco Paolo Russo Giacomo Germani Marco Senzolo Alessandro Vitale Umberto Cillo Patrizia Burra 《肝癌研究(英文版)》2021,7(1):38-49
Background: The impact of sarcopenia on the outcome of patients with cirrhosis who undergo liver transplantation (LT) has been analysed in heterogeneous cohorts... 相似文献
10.
Tasca A Cacciola A Ferrarese P Ioverno E Visonà E Bernardi C Nobile M Giannini S 《Urology》2002,59(6):865-9; discussion 869
Objectives. To verify whether alterations in bone density and turnover in patients with calcium nephrolithiasis and hypercalciuria are observable in various subgroups of patients divided according to the pathogenesis of the hypercalciuria.Methods. Seventy patients with calcium nephrolithiasis and idiopathic hypercalciuria, 19 to 64 years old, were assessed for spine and femur mineral metabolism and bone density using a Dexa evaluation system. After a low calcium diet, the subjects were classified into two groups: fasting hypercalciuria (FH, 39 patients) and absorptive hypercalciuria (AH, 31 patients).Results. Only in the patients with FH was the lumbar spine bone density lower than in the controls (P <0.001). Also, only the patients with FH had higher bone alkaline phosphatase and urinary hydroxyproline levels than the control group (P <0.005 and <0.015, respectively). The blood pH levels were lower, even though within the normal range, in the hypercalciuric patients than in the controls (P <0.01). There was a negative correlation between the urinary hydroxyproline level and lumbar spine and femoral neck density in patients with FH (P <0.001 and <0.005, respectively), and the blood pH correlated positively with the lumbar spine bone density.Conclusions. Altered bone metabolism and overall bone loss were found only in the patients with FH. Overloading of acid valences, perhaps of dietary origin, could be the pathogenic factor responsible. 相似文献