全文获取类型
收费全文 | 85篇 |
免费 | 1篇 |
国内免费 | 3篇 |
专业分类
儿科学 | 12篇 |
妇产科学 | 1篇 |
基础医学 | 1篇 |
口腔科学 | 2篇 |
临床医学 | 6篇 |
内科学 | 32篇 |
神经病学 | 2篇 |
特种医学 | 10篇 |
外科学 | 6篇 |
综合类 | 6篇 |
预防医学 | 2篇 |
眼科学 | 2篇 |
药学 | 5篇 |
肿瘤学 | 2篇 |
出版年
2021年 | 1篇 |
2017年 | 1篇 |
2015年 | 4篇 |
2014年 | 2篇 |
2013年 | 4篇 |
2012年 | 2篇 |
2011年 | 2篇 |
2010年 | 4篇 |
2009年 | 6篇 |
2008年 | 4篇 |
2007年 | 1篇 |
2006年 | 3篇 |
2004年 | 2篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1998年 | 2篇 |
1997年 | 3篇 |
1996年 | 10篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 3篇 |
1987年 | 4篇 |
1986年 | 3篇 |
1985年 | 4篇 |
1982年 | 5篇 |
1980年 | 1篇 |
1978年 | 2篇 |
1976年 | 1篇 |
排序方式: 共有89条查询结果,搜索用时 0 毫秒
1.
The effect of thrombopoietin on the proliferation and differentiation of murine hematopoietic stem cells 总被引:15,自引:16,他引:15
Sitnicka E; Lin N; Priestley GV; Fox N; Broudy VC; Wolf NS; Kaushansky K 《Blood》1996,87(12):4998-5005
In this study, we explored whether thrombopoietin (Tpo) has a direct in vitro effect on the proliferation and differentiation of long-term repopulating hematopoietic stem cells (LTR-HSC). We previously reported a cell separation method that uses the fluorescence-activated cell sorter selection of low Hoescht 33342/low Rhodamine 123 (low Ho/low Rh) fluorescence cell fractions that are highly enriched for LTR-HSC and can reconstitute lethally irradiated recipients with fewer than 20 cells. Low Ho/low Rh cells clone with high proliferative potential in vitro in the presence of stem cell factor (SCF) + interleukin-3 (IL-3) + IL-6 (90% to 100% HPP-CFC). Tpo alone did not induce proliferation of these low Ho/low Rh cells. However, in combination with SCF or IL-3, Tpo had several synergistic effects on cell proliferation. When Tpo was added to single growth factors (either SCF or IL-3 or the combination of both), the time required for the first cell division of low Ho/low Rh cells was significantly shortened and their cloning efficiency increased substantially. Moreover, the subsequent clonal expansion at the early time points of culture was significantly augmented by Tpo. Low Ho/low Rh cells, when assayed in agar directly after sorting, did not form megakaryocyte colonies in any growth condition tested. Several days of culture in the presence of multiple cytokines were required to obtain colony-forming units-megakaryocyte (CFU-Mk). In contrast, more differentiated, low Ho/high Rh cells, previously shown to contain short- term repopulating hematopoietic stem cells (STR-HSC), were able to form megakaryocyte colonies in agar when cultured in Tpo alone directly after sorting. These data establish that Tpo acts directly on primitive hematopoietic stem cells selected using the Ho/Rh method, but this effect is dependent on the presence of pluripotent cytokines. These cells subsequently differentiate into CFU-Mk, which are capable of responding to Tpo alone. Together with the results of previous reports of its effects on erythroid progenitors, these results suggest that the effects of Tpo on hematopoiesis are greater than initially anticipated. 相似文献
2.
GV Ramesh Prasad Marcel Ruzicka Kevin D Burns Sheldon W Tobe Marcel Lebel 《The Canadian journal of cardiology》2009,25(5):309-314
For the first time, the Canadian Hypertension Education Program has studied the evidence supporting blood pressure control in people requiring renal replacement therapy for end-stage kidney disease, including those on dialysis and with renal transplants. According to the Canadian Organ Replacement Registry’s 2008 annual report, there were an estimated 33,832 people with end-stage renal disease in Canada at the end of 2006, an increase of 69.7% since 1997. Of these, 20,465 were on dialysis and 13,367 were living with a functioning kidney transplant. Thus, it is becoming more likely that primary care practitioners will be helping to care for these complex patients. With the lack of large controlled clinical trials, the consensus recommendation based on interpretation of the existing literature is that blood pressure should be lowered to below 140/90 mmHg in hypertensive patients on renal replacement therapy and to below 130/80 mmHg for renal transplant patients with diabetes or chronic kidney disease. 相似文献
3.
The coeliac iceberg in Italy. A multicentre antigliadin antibodies screening for coeliac disease in school-age subjects 总被引:8,自引:0,他引:8
C Catassi E Fabiani IM Rätsch GV Coppa PL Giorgi R Pierdomenico S Alessandrini G Iwanejko R Domenici E Mei A Miano M Marani G Bottaro M Spina M Dotti A Montanelli M Barbato F Viola R Lazzari M Vallini G Guariso M Plebani F Cataldo G Traverso C Ughi G Chiaravalloti M Baldassarre P Scarcella F Bascietto L Ceglie A Valenti P Paolucci M Caradonna E Bravi A Ventura 《Acta paediatrica (Oslo, Norway : 1992)》1996,85(S412):29-35
Background : Recent studies suggest that coeliac disease (CD) is one of the commonest, life-long disorders in Italy. The aims of this multicentre work were: (a) to establish the prevalence of CD on a nationwide basis; and (b) to characterize the CD clinical spectrum in Italy. Patients and methods : Fifteen centres screened 17201 students aged 6–15 years (68.6% of the eligible population) by the combined determination of serum IgG- and IgA-antigliadin antibody (AGA) test; 1289 (7.5%) were IgG and/or IgA-AGA positive and were recalled for the second-level investigation; 111 of them met the criteria for the intestinal biopsy: IgA-AGA positivity and/or AEA positivity or IgG-AGA positivity plus serum IgA deficiency. Results : Intestinal biopsy was performed on 98 of the 111 subjects. CD was diagnosed in 82 subjects (75 biopsy proven, 7 not biopsied but with associated AGA and AEA positivity). Most of the screening-detected coeliac patients showed low-grade intensity illness often associated with decreased psychophysical well-being. There were two AEA negative cases with associated CD and IgA deficiency. The prevalence of undiagnosed CD was 4.77 × 1000 (95% CI 3.79–5.91), 1 in 210 subjects. The overall prevalence of CD, including known CD cases, was 5.44 × 1000 (95% CI 4.57–6.44), 1 in 184 subjects. The ratio of known to undiagnosed CD cases was 1 in 7. Conclusions : These findings confirm that, in Italy, CD is one of the most common chronic disorders showing a wide and heterogeneous clinical spectrum. Most CD cases remain undiagnosed unless actively searched. 相似文献
4.
Paltiel HJ; O'Gorman AM; Meagher-Villemure K; Rosenblatt B; Silver K; Watters GV 《Radiology》1987,162(1):115
5.
Prevalence and characteristics of brittle diabetes in Britain 总被引:3,自引:0,他引:3
Gill GV; Lucas S; Kent LA 《QJM : monthly journal of the Association of Physicians》1996,89(11):839-843
We investigated the prevalence and characteristics of 'brittle diabetes',
defined as insulin-dependent diabetes mellitus associated with glycaemic
instability of any type, leading to life disruption with recurrent and/or
prolonged hospitalizations. A questionnaire was sent to all physicians and
paediatricians running diabetic clinics in the UK, from lists held at the
British Diabetic Association. A total of 414 brittle patients were reported
(72% questionnaire return). Most were young (mean age +/- SD was 26 +/- 15
years), though there was a small peak at ages 60-70 years. There was an
excess of females (66%) and overall clinic prevalence was 1.2 per 1000
diabetic patients and 2.9 per 1000 insulin-treated diabetic patients. On
average, there was 1.0 brittle patient per diabetic clinic. The most common
form of brittleness was recurrent ketoacidosis (59%), with 17% having
predominant hypoglycaemia, and 24% mixed instability. Female excess was
highest and mean age lowest in the recurrent ketoacidosis group, whilst the
reverse was true for those with recurrent hypoglycaemia. Causes of
brittleness were offered by 58% of consultants, and most (93%) considered
various psychosocial problems as likely underlying factors. We conclude
that brittle diabetes is a small but significant problem, currently
affecting about 1 per 1000 diabetic patients. Most, but by no means all,
are young females--often with recurrent ketoacidosis. Older age groups are
more likely to have recurrent hypoglycaemic or mixed types of brittleness.
Perceived causes of brittleness are usually psychosocial.
相似文献
6.
SUMMARY Analysis of the age of onset of diabetes amongst insulin-treatedpatients in a large African diabetic clinic revealed a bimodaltype of distribution, 23 per cent having an age of onset before30 years and 77 per cent with onset at 30 years of age. All66 of the young insulin-treated group (21.7±4.8 years(mean±1 SD)), and a random selection of 50 older insulin-treatedpatients (49.7±10 years), were studied. The older groupwere better controlled (HbA1 8.4±1.7 per cent vs. 10.8±2.6per cent, p<0.001), on lower doses of insulin (49±23vs. 71±23 u/day, p<0.001) and had higher body massindex (26.0±5.6 vs. 21.8±3.5, p<0.001). SerumC-peptide (0.24±0.15 vs. 0.07±0.10 nmol/l, p<0.0001),and C-peptide/glucose ratio (2.57±2.65 vs. 0.56+0.98nmol/mmolx 102, p<0.001) were very significantly higher inolder patients. Patients with later onset disease thus had betterpreservation of pancreatic function, higher body mass indexand better glycaemic control on lower doses of insulin. Thesefeatures suggest that older insulin-treated patients could infact be Type 2 or non-insulin dependent patients,and the condition may be controllable with diet and/or oralhypoglycaemic agents, at least in some. 相似文献
7.
Correlation of drug sensitivity in vitro with clinical responses in childhood acute myeloid leukemia 总被引:1,自引:0,他引:1
Clonogenic cells from 41 children with newly diagnosed acute myeloid leukemia (AML) were tested in vitro for their sensitivity to cytarabine (Ara-C) and daunorubicin (DNR). The findings were then compared with the patients' responses to induction chemotherapy that uniformly included Ara-C and DNR. Light-density marrow cells were incubated with either or both drugs for one hour and cultured over leukocyte feeder layers; clusters and colonies were scored on days 7, 10, and 14. Only the percentage of cell kill in the presence of 1.8 mumol/L DNR was significantly associated with responses to induction therapy: median of 45% (range, 0% to 98%) for patients achieving complete remission v 16% (range, 4% to 23%) for nonresponders (P = .007). The relationship between clonogenic cell kill less than or equal to 23% and clinical responses was striking. Of the 11 evaluable patients with in vitro findings in this category, ten either failed induction therapy or relapsed within 1 year after attaining remission. Kaplan-Meier analysis of relapse-free survival times indicated longer durations of remission for patients whose blast cells showed increased sensitivity in vitro to Ara-C alone, DNR alone, or a combination of the two agents. Seven of 11 patients with cell kills of greater than or equal to 49% in the presence of 1.25 mumol/L Ara-C remain free of leukemia, compared with only one of 12 whose cells were less sensitive to the drug (P = .006). We conclude that the in vitro sensitivity of clonogenic leukemic progenitors to DNR and Ara-C correlates with treatment outcome in children with newly diagnosed AML. 相似文献
8.
AbstractBackground: This study examined parents’ developmental concerns for their children within the context of systems of care in Malaysia.Methods: Focus groups and interviews were conducted in peninsular Malaysia and Borneo.Results: Parents’ perceptions of developmental delay stemmed from three sources: the cultural, resource, and the social environments.Conclusion: There is a need to develop a medical support system in Malaysia that considers a life-course perspective, including prenatal care, screening/diagnosis, and services. This system should embrace a family-centered approach to diagnosis, referral, intervention, and support with sensitivity to cultural beliefs, family preferences, and barriers to care.
- Implications for Rehabilitation
Parental perceptions of disability affect the strategies they use to cope.
This research found that cultural conceptions of disability, available resources, and social support affect parental perceptions of disability.
The resource environment in Malaysia significantly restricts parents’ ability to cope with their child's disability.
This research recommended that the medical system of Malaysia develops a life-course perspective to disability to provide a range of care for children with disabilities including prenatal care, screening and rehabilitation or coping services.
9.
GV Coppa P Pierani L Zampini I Carloni A Carlucci O Gabrielli 《Acta paediatrica (Oslo, Norway : 1992)》1999,88(S430):89-94
Twenty-one oligosaccharides of human milk were quantified by high-performance anion-exchange chromatography. Milk samples were collected from 18 mothers during the first 3 mo of lactation. The data show that the highest amount of all oligosaccharides is present at day 4 postpartum (20 g l−1 ) and then decreases by about 20% at day 30 of lactation. The protective role played by these substances against different infectious agents, in different organs and systems of the breastfed baby, is emphasized. 相似文献
10.
AE Scaramuzza A De Palma C Mameli D Spiri L Santoro GV Zuccotti 《Acta paediatrica (Oslo, Norway : 1992)》2010,99(8):1237-1241
Aim: The aim of the student is to assess whether adolescents with type 1 diabetes mellitus (T1DM) in Italy differ from their healthy peers in regard to risky behaviour. Methods: Data were collected from 215 patients, aged 14 ± 2 years with a mean disease duration of 7 ± 5 years. The control group was comprised of 464 healthy adolescents recruited among high school students. Each patient completed an anonymous confidential questionnaire to determine the prevalence of sexual behaviour, alcohol and tobacco consumption, illicit drug use, and, among patients with diabetes and frequency of mismanagement related to diabetes care. Results: Compared with controls, subjects with diabetes showed a similar rate of sexual intercourse among males and lower rates among females (34.8% vs 35.5%, p NS and 29.4% vs 41.4%, p < 0.05, respectively). Males in the diabetes group reported a higher rate of tobacco use, whereas females showed similar or higher rates of use for every illicit drug studied. Among patients with diabetes, those who are engaged in risky behaviour showed a higher rate of treatment mismanagement (76% vs 34%, p < 0.01). Conclusion: Adolescents with T1DM are as likely as their healthy peers to engage in risky behaviour, indicating the potential benefit of anticipatory guidance concerning glycaemic control and increased risk of acute and chronic complications. 相似文献