钉螺对不同宿主源性日本血吸虫毛蚴的易感性

目的了解钉螺对不同终宿主源性日本血吸虫毛蚴的易感性差异。方法收集家兔、水牛和小白鼠排出的虫卵,孵出毛蚴,以毛蚴和钉螺5：1的比例感染同一地钉螺。同时设对照组,观察各组钉螺感染率、死亡率。结果实验组家兔、水牛和小白鼠排出虫卵孵出毛蚴感染钉螺,获得的阳性率分别为1.42%、8.67%和19.87%,钉螺死亡率分别为29.5%、13.5%和24.5%;对照组钉螺阳性率分别为2.63%、2.02%和11.66%,死亡率分别为24.0%、49.5%和18.5%。结论不同宿主源日本血吸虫毛蚴对钉螺易感性存在差异。     

经脐单孔腹腔镜肝切除：首例报道

Transumbilical single-incision laparoscopic surgeries have attracted the attention of surgeon. Here we report a patient with multiple hepatic hemagiomas and symptomatic cholelithiasis who underwent laparoscopic left lateral hepatecomy and left hepatic hemangioma enucleation with single incision followed by cholecystectomy. The duration of the operation was 155 minutes and the blood loss was 100 ml. There were no complications during or after the treatment. This surgical treatment yields a good cosmetic effect and rapid recovery.

     

藐小棘隙吸虫病流行病学研究Ⅱ．中间宿主感染情况及其季节消长

１９９１～１９９３年对和县陈桥洲村进行了藐小棘隙吸虫中间宿主感染情况调查。首次报告了铜锈环棱螺是藐小棘隙吸虫第一中间宿主，其自然感染率呈明显的季节性变化，７月下旬至１２月上旬可检到阳性螺，以９～１１月感染率最高。检查第二中间宿主淡水鱼２０种，１８７６尾，其中１８种检出本虫囊蚴，平均感染率为８３．７０％，淡水鱼各月感染率间无显著差异，但感染率以１１月至翌年４月最高。     

Diluted povidone-iodine inhibits tumor growth through apoptosis-induction and suppression of SOD activity

Povidone-iodine (PVP-I) is widely used in clinical practice as an antiseptic and flushing agent after surgery to remove a tumor. Our present study was designed to determine whether diluted PVP-I is cytotoxic to colon cancer cells and ascetic tumor cells in?vitro and to examine its antitumor effects in?vivo. In?vitro, CT26 and H22 cells treated with different concentrations of diluted PVP-I (0-1.56?μg/ml) were analyzed using the mononuclear cell direct cytotoxicity assay (MTT) and a flow cytometry assay. In?vivo, Balb/c mice injected in the abdominal cavity with CT26 cells or H22 cells were treated intraperitoneally with different concentrations of PVP-I (0-312.5?μg/mouse), cisplatin (40?mg/kg) or 5'-FU (30?mg/kg) or left untreated. In?vitro, the studies demonstrated the antiproliferative and significant apoptosis-inducing effects of PVP-I in a dose- and time-dependent manner. In?vivo, PVP-I significantly repressed the growth of H22 and CT26 cells in Balb/c mice compared to controls. To explore the mechanism of the antitumor effect of PVP-I, the superoxide dismutase (SOD) activity of ascites extracted from a mouse model and the supernatant of CT26 cells was detected by an SOD kit. The activity of SOD was significantly inhibited in the experimental groups. Taken together, our data suggest that PVP-I exhibits a strong inhibitory effect on tumor growth in colon cancer (CT26) and hepatoma (H22) resulting from apoptosis, both in?vitro and in?vivo, suggesting a new potential therapeutic approach after tumor excision surgery to colon cancer and hepatoma.     

三氧化二砷诱导NB4细胞凋亡和环氧合酶-2基因表达研究

本研究探讨三氧化二砷(As2O3)诱导NB4细胞凋亡过程中线粒体膜电位的改变及环氧合酶-2的表达变化。利用免疫荧光显微镜、DNA电泳等观察As2O3诱导NB4细胞凋亡过程中NB4细胞的形态学改变,流式细胞术检测NB4细胞凋亡率及线粒体膜电位改变,Western blot分析环氧合酶-2的蛋白质表达水平变化。结果表明,经2μmol/L As2O3处理NB4细胞48小时后,荧光显微镜显示NB4细胞出现核致密浓染、染色质固缩及片断化断裂,甚至呈碎片状;提取DNA进行琼脂糖电泳显示典型DNA Ladder现象。流式细胞术分析发现,NB4细胞经3μmol/L As2O3处理48小时后细胞凋亡率为33.34%,As2O3浓度越高,NB4细胞凋亡率越高;以0.5、1、2、4及8μmol/L As2O3分别处理NB4细胞48小时后,线粒体膜电位分别下降12.8%、21.6%、66.9%、83.7%和83.8%;Western blot检测显示,环氧合酶-2在实验组的表达明显低于对照组,且随着As2O3浓度的升高,环氧合酶-2的表达逐渐降低,二者呈显著的负相关关系。结论 :As2O3能够有效诱导NB4细胞凋亡,且在一定浓度范围内具有量效关系;线粒体膜电位下降与As2O3诱导的NB4细胞凋亡有关;环氧合酶-2可能参与了As2O3诱导的NB4细胞凋亡过程。