Cordycepin prevents oxidative stress-induced inhibition of osteogenesis

Oxidative stress is known to be involved in impairment of osteogenesis and age-related osteoporosis. Cordycepin is one of the major bioactive components of Cordyceps militaris that has been shown to exert antioxidant and anti-inflammatory activities. However, there are few reports available regarding the effects of cordycepin on osteogenesis and the underlying mechanism. In this study, we investigated the potential osteoprotective effects of cordycepin and its mechanism systematically using both in vitro model as well as in vivo mouse models. We discovered that hydrogen peroxide (H₂O₂) induced inhibition of osteogenesis which was rescued by cordycepin treatment in human bone marrow mesenchymal stem cells (BM-MSCs). Cordycepin exerted its protective effects partially by increasing or decreasing expression of osteogenic and osteoclastogenesis marker genes. Treatment with cordycepin increased Wnt-related genes'' expression whereas supplementation of Wnt pathway inhibitor reversed its protective effects. In addition, administration of cordycepin promoted osteogenic differentiation of BM-MSCs by reducing oxidative stress in both ovariectomized and aged animal models. Taken together, these results support the protective effects of cordycepin on oxidative stress induced inhibition of osteogenesis by activation of Wnt pathway.     

脑出血术后的机械通气治疗

目的 探讨脑出血术后患者机械通气治疗的策略及其疗效。方法 脑出血术后患者中，符合上机指征的有53例，在常规治疗的基础上，予以机械通气。分析治疗前后呼吸指标的变化。结果 脑出血术后，给予机械通气治疗2h后，与机械通气前比较，各项指标包括呼吸频率RR、氧分压脚晚、二氧化碳分压PaCO2、血氧饱和度SpO2均有改善，并有统计学意义（P〈0．05）。经过综合治疗后，好转出院18例（33．96％），自动出院16例（30．19％），死亡19例（35．85％）。结论 脑出血术后患者，合理机械通气治疗（包括过度通气等）可明显改善患者的呼吸功能、减少死亡率。     

Initial symptoms of vertebrobasilar artery occlusions and the outcomes after endovascular treatment

Journal of Neurology - Acute vertebrobasilar artery occlusion (VBAO) is a devastating disease in stroke patients. This study was aimed to identify the initial symptoms of patients with acute VBAO...     

逆转录病毒的高效包装及转基因鸡技术平台的建立

转基因鸡及输卵管生物反应器正日益成为生物领域研究的热点之一,当前研究转基因鸡最成功的方法即为逆转录病毒介导法,但目前国内尚无系统报道逆转录病毒法制备转基因鸡的平台技术。为加快国内这一领域的研究步伐,本文较详尽的介绍了从逆转录病毒包装、竞争法包装泛嗜性VSV-G病毒、病毒包装条件的优化、病毒的浓缩、辅助病毒检测、鸡胚盘下腔注射等方法技术,并分别在体外鸡胚胎原代成肌细胞(CFM)和体内鸡胚中检测目的标记基因GFP的病毒感染整合效率,为今后转基因鸡的研究提供了一个实用的技术平台。     

Treatment of middle third humeral shaft fractures: minimal invasive plate osteosynthesis versus expandable nailing

The purpose of this study is to compare the results of middle third humeral shaft fractures treated with minimally invasive plate osteosynthesis (MIPO) with those treated with an expandable nail technique. Thirty-four patients with middle third humeral shaft fractures were retrospectively analyzed and divided into two groups: group A included 15 patients treated by MIPO and group B included 19 patients treated with an expandable intramedullary nail. The operation time, postoperative complications, time to fracture union, the UCLA End-Result score of the shoulder, and Mayo elbow performance index (MEPI) were recorded and compared. The mean operation time in group A was 108.3 ± 28.7 min and 85.0 ± 35.7 min in group B, a difference of 23.3 (27.4%) minutes (P < 0.05). There were no cases of iatrogenic radial nerve palsy or superficial infection in either group. All fractures united. The mean time to fracture union in group A was 16.1 ± 6.0 (8–32)weeks, versus 15.0 ± 2.5 (10–21) weeks in group B (P > 0.05). One case of delayed union was identified in group A. The mean UCLA End-Result score in group A was 34.2 ± 1.1 points (31–35 points)and 31.8 ± 2.6 points (26–35) in group B (t = 3.318 P = 0.002, P < 0.05). All the patients had excellent elbow functions. In surgical treatment of middle third humeral shaft fractures, MIPO appeared to offer advantages in terms of better shoulder and no iatrogenic radial nerve injuries, although technically more difficult and time consuming.     

经前侧入路手术治疗骨盆新月型骨折

目的探讨经前侧入路治疗骨盆新月型骨折的疗效。方法 2007年6月-2009年12月,采用经前侧入路治疗骨盆新月型骨折18例,其中男11例,女7例;年龄19～52岁,平均32岁。左侧10例,右侧8例。交通事故伤10例,高处坠落伤5例,重物压伤3例;均为侧方挤压暴力。受伤至手术时间3～22 d,平均7.8 d。按照Matta等的方法测量术前骨盆后环骨折移位为5～24 mm,平均13.7 mm。记录手术时间、术中失血量、手术纠正骨盆后环骨折移位、骨折愈合时间、Hannover骨盆评分。结果手术时间110～230 min,平均175 min;术中失血量300～1 100 mL,平均561.7 mL。术后24 h骨盆后环骨折移位0～3 mm,平均1.2 mm;手术纠正移位4～23 mm,平均12.6 mm。术中无腰骶神经根损伤患者。术后2例切口浅表感染,经敞开切口引流、换药及应用抗生素1周,无炎性反应后缝合治愈;其余患者切口均Ⅰ期愈合。15例获随访,随访时间13～22个月,平均16.1个月。X线片示患者骨折均愈合,愈合时间3～4个月,平均3.6个月。未出现骨折复位丢失,无内固定物移位、断裂等并发症发生。按照Hannover骨盆评分标准:临床症状恢复获优10例(66.7%),良5例(33.3%);重返社会能力方面,完全重返13例(86.7%),部分重返2例(13.3%)。结论经前侧入路手术治疗骨盆新月型骨折可以取得良好疗效。     

结直肠癌术前区域动脉灌注化疗的临床病理研究

目的探讨结直肠癌术前区域动脉灌注化疗对临床病理特征的作用及其可行性。方法以Seldinger 方法插管,选择性区域动脉造影灌注化疗结直肠癌30例。结果所有病例(30/30)均发现沿血管周围的癌细胞发生核固缩、碎裂、胞浆凝固、坏死,细胞间质水肿、纤维增生、炎性细胞浸润,血管出现内膜增生、血管炎及血栓形成。结论术前选择性区域动脉灌注化疗对结直肠癌组织学改变显著,临床症状改善,对结直肠癌治疗具有积极的作用。     

介入血运重建改善颈动脉次全闭塞伴轻卒中患者认知障碍的研究

目的研究颈内动脉次全闭塞伴有轻卒中患者认知障碍的特点,明确介入颈动脉血运重建对这类患者认知障碍的改善作用。 方法续贯纳入济宁市第一人民医院神经内科自2016年2月至2019年2月住院的一侧颈动脉次全闭塞伴有轻卒中患者,住院期间完成介入颈动脉血运重建手术,分别在术前和术后3个月接受MMSE评分和MoCA评分检查。 结果共纳入33例患者,所有患者住院期间成功接受了介入颈动脉血运重建。患者术前33例患者在接受MoCA评分时都提示认知功能下降（19.58±1.54）,而MMSE评分未发现有认知功能下降（26.88±0.74）。33例接受介入颈动脉血运重建患者术后3个月的MMSE和MoCA评分较术前均有提高,而且差异具有统计学意义（P<0.05）。 结论颈内动脉次全闭塞伴有轻卒中患者的认知障碍的特点是MoCA评分较MMSE评分更敏感,能够发现早期认知功能障碍。介入血运重建技术能够改善颈内动脉次全闭塞伴有轻卒中患者的认知功能障碍。