Characterization of twenty-four polymorphic microsatellite loci of Rasbora borapetensis

Twenty-four microsatellite loci were isolated and characterized from the genome of Rasbora borapetensis. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic with allele numbers ranging from 2 to 27, observed heterozygosity from 0.031 to 1.000 and expected heterozygosity from 0.031 to 0.965. All loci conformed to the Hardy–Weinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci will enhance future investigations on the genetic differentiation and structure of populations of Rasbora borapetensis.     

Development and characterization of 29 polymorphic microsatellite loci for Esomus metallicus

Twenty-nine microsatellite loci were isolated and characterized from the genome of Esomus metallicus. Flanking polymerase chain reaction primers were designed and used to amplify these loci in 32 individuals. All loci were polymorphic; with allele numbers ranged from 2 to 27, observed heterozygosity from 0.031 to 0.969 and expected heterozygosity from 0.031 to 0.957. All conformed to Hardy?CWeinberg equilibrium and no evidence of null alleles was observed. Pairwise comparisons between alleles did not detect any cases of linkage disequilibrium. The high level of polymorphisms observed in these microsatellite loci should enhance future investigation of the genetic differentiation and structure of populations of E. metallicus.     

Isolation and characterization of 40 polymorphic microsatellite markers from Parambassis siamensis

We isolated and characterized a total of 40 polymorphic microsatellite loci from the genome of Parambassis siamensis. Flanking Polymerase Chain Reaction primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 32 individuals ranging from 2 to 29. The observed heterozygosity ranged from 0.063 to 0.969, while the expected heterozygosity varied from 0.062 to 0.960. All loci conformed to Hardy?CWeinberg equilibrium, none of the loci combinations showed significant linkage disequilibrium, and no loci showed evidence of null alleles. These 40 microsatellite loci should be useful for conducting future investigations into the genetic differentiation and structure of populations of P. siamensis.     

Development of nineteen novel polymorphic microsatellite loci of Clupeichthys aesarnensis

We developed and characterized a total of 19 novel polymorphic microsatellite loci from the genome of Clupeichthys aesarnensis. Flanking PCR primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 30 individuals ranging from two to 15. The observed heterozygosity ranged from 0.033 to 0.933, while the expected heterozygosity varied from 0.033 to 0.898. All loci conformed to the Hardy–Weinberg equilibrium, none of the loci combinations showed significant linkage disequilibrium, and no loci showed evidence of null alleles. These 19 microsatellite loci can be useful for conducting future investigations into the genetic differentiation and structure of populations of C. aesarnensis.