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Sukjae Joshua Kang Bong-Kiun Kaang 《The Korean journal of physiology & pharmacology》2016,20(6):557-564
Metabotropic glutamate receptor (mGluR)-dependent long-term depression (LTD), a type of synaptic plasticity, is characterized by a reduction in the synaptic response, mainly at the excitatory synapses of the neurons. The hippocampus and the cerebellum have been the most extensively studied regions in mGluR-dependent LTD, and Group 1 mGluR has been reported to be mainly involved in this synaptic LTD at excitatory synapses. However, mGluR-dependent LTD in other brain regions may be involved in the specific behaviors or diseases. In this paper, we focus on five cortical regions and review the literature that implicates their contribution to the pathogenesis of several behaviors and specific conditions associated with mGluR-dependent LTD. 相似文献
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Kim JI Lee HR Sim SE Baek J Yu NK Choi JH Ko HG Lee YS Park SW Kwak C Ahn SJ Choi SY Kim H Kim KH Backx PH Bradley CA Kim E Jang DJ Lee K Kim SJ Zhuo M Collingridge GL Kaang BK 《Nature neuroscience》2011,14(11):1447-1454
Phosphatidylinositol 3-kinase (PI3K) has been implicated in synaptic plasticity and other neural functions in the brain. However, the role of individual PI3K isoforms in the brain is unclear. We investigated the role of PI3Kγ in hippocampal-dependent synaptic plasticity and cognitive functions. We found that PI3Kγ has a crucial and specific role in NMDA receptor (NMDAR)-mediated synaptic plasticity at mouse Schaffer collateral-commissural synapses. Both genetic deletion and pharmacological inhibition of PI3Kγ disrupted NMDAR long-term depression (LTD) while leaving other forms of synaptic plasticity intact. Accompanying this physiological deficit, the impairment of NMDAR LTD by PI3Kγ blockade was specifically correlated with deficits in behavioral flexibility. These findings suggest that a specific PI3K isoform, PI3Kγ, is critical for NMDAR LTD and some forms of cognitive function. Thus, individual isoforms of PI3Ks may have distinct roles in different types of synaptic plasticity and may therefore influence various kinds of behavior. 相似文献
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We determined in detail the primary structure requirements for green fluorescence of the jellyfish green fluorescent protein (GFP) and of its improved mutants (S65T and I167T). We performed a deletion mapping in combination with fluorescence-activated cell sorting (FACS) and spectrofluorometry. This showed that deletion of up to nine amino acids at the C-terminal end of GFP had no deleterious effect on the fluorescent activity of GFP; in fact the deletion increased intensity of fluorescence. Further truncation of up to 11 amino acids resulted in partial impairment in maximal fluorescence. The GFP fluorescence was completely disrupted when more than 12 amino acids were deleted out of the C-terminal. Truncated mutants or their fusion genes with lacZ emitted fluorescence when plasmids encoding them were introduced by microinjection into Aplysia neurons. 相似文献
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Chuljung Kwak Sue-Hyun Lee Bong-Kiun Kaang 《The Korean journal of physiology & pharmacology》2009,13(5):357-360
It is hypothesized that a number of environmental factors affect animals'' behavior. Without controlling these variables, it is very hard for researchers to get not only reliable, but replicable data from various behavioral experiments testing animals'' cognitive as well as emotional functions. For example, laboratory mice which had restricted environment showed different synaptic potentiation properties with wild mice (Zhao MG et al., 2009). While performing behavioral experiments, however, it is sometimes inevitable that the researcher changes the animals'' environments, as by switching the cages in which experimental animals are housed and separating animals raised together into small experimental groups. In this study, we investigated the effect of environmental changes on mice''s emotional behaviors by socially isolating them or reducing the size of their cage. We found that social isolation selectively increases the animals'' levels of anxiety, while leaving depression-like behaviors unchanged. On the other hand, alteration of the housing dimensions affected neither their anxiety levels nor their depression-like behaviors. These results suggest that environmental variables may have a prominent impact on experimental animals'' emotional behaviors and possibly their psychological states, leading to bias in the behavioral data produced from experiments. 相似文献
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We assessed the role of a non-inactivating K+ channel (aKv5.1) in the resting potential by overexpressing this channel by heat shock in the neurons. A reporter gene lacZ linked to a promoter region spanning from the -285 to the +88 base of the rat HSP70ib gene was induced 62.5-fold when this DNA construct was microinjected into the neurons of the marine mollusk Aplysia and treated with heat shock at 30 degrees C for 3 h. Using this efficient induction system, we induced the expression of aKv5.1 by heat shock in cultured, electrically silent neurons of Aplysia and examined its effect on the resting potential. The channel expression increased the resting potential by approximately 10 mV. This increase was specific to heat shock induction and abolished by treatment with TEA, a specific K+ channel blocker. These results provide the direct evidence that a low voltage-activated, non-inactivating K+ channel can contribute to the resting potential. 相似文献
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Cofilin expression induces cofilin-actin rod formation and disrupts synaptic structure and function in Aplysia synapses
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Jang DH Han JH Lee SH Lee YS Park H Lee SH Kim H Kaang BK 《Proceedings of the National Academy of Sciences of the United States of America》2005,102(44):16072-16077
Cofilin-actin rods are inclusion-like structures that are induced by certain chemical or physical stresses in cultured cells, and the rods formed in neurons are thought to be associated with neurodegeneration. Here, we cloned an Aplysia cofilin homolog and overexpressed it in cultured neurons. Overexpressed cofilin formed rod-like structures that included actin. The overall neuronal morphology was unaffected by cofilin overexpression; however, a decrease in number of synaptic varicosities was observed. Consistent with this structural change by cofilin overexpression, the synaptic strength was reduced, and furthermore, the long-term facilitation elicited by repeated pulses of 5-hydroxytryptamine was impaired in sensory-to-motor synapses. However, cofilin overexpression did not induce programmed cell death. These findings suggest that the formation of cofilin-actin rod-like structures can lead to neurodegeneration, and this might be a mechanism of rundown of neuronal and synaptic function without cell death in neurodegenerative diseases. 相似文献
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Chang DJ Li XC Lee YS Kim HK Kim US Cho NJ Lo X Weiss KR Kandel ER Kaang BK 《Proceedings of the National Academy of Sciences of the United States of America》2000,97(4):1829-1834
Short-term behavioral sensitization of the gill-withdrawal reflex after tail stimuli in Aplysia leads to an enhancement of the connections between sensory and motor neurons of this reflex. Both behavioral sensitization and enhancement of the connection between sensory and motor neurons are importantly mediated by serotonin. Serotonin activates two types of receptors in the sensory neurons, one of which is coupled to the cAMP/protein kinase A (PKA) pathway and the other to the inositol triphosphate/protein kinase C (PKC) pathway. Here we describe a genetic approach to assessing the isolated contribution of the PKA pathway to short-term facilitation. We have cloned from Aplysia an octopamine receptor gene, Ap oa(1), that couples selectively to the cAMP/PKA pathway. We have ectopically expressed this receptor in Aplysia sensory neurons of the pleural ganglia, where it is not normally expressed. Activation of this receptor by octopamine stimulates all four presynaptic events involved in short-term synaptic facilitation that are normally produced by serotonin: (i) membrane depolarization; (ii) increased membrane excitability; (iii) increased spike duration; and (iv) presynaptic facilitation. These results indicate that the cAMP/PKA pathway alone is sufficient to produce all the features of presynaptic facilitation. 相似文献