铁调节蛋白2基因多态性与阿尔茨海默病及血管性痴呆的相关分析

Objective To evaluate the association of 2616c/T polymorphism in iron regulatory protein 2(IRP2)gene with Alzheimer disease(AD)and Vascular dementia(VD).Methods In this study,281 patients with AD,60 with VD,and 285 normal aged were recruited.The 2616C/T polymorphism in IRP2 gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism.And the cognitive function was assessed with the Mini-Mental State Examination(MMSE).Results (1)No significant difieFences were demonstrated in IRP2 genotype or allele frequencies between AD patients and controls(χ2=2.46,P=0.292;χ2=2.17,P=0.141 respectively).However,when AD patients were stratified by disease severity.the frequency of T allele carriers in the moderate to severe AD patients was 78.0%,significantly higher than that in controls(69.8%;χ2=4.106,P＜0.05).Logistic regression analysis demonstrated that the age-,sex-and ApoE-adiusted OR of modcrate to severe AD patient with T allele was 1.62(95% CI=1.03-2.54).The frequency of T allele carriers or T allele in VD patients was higher than that of controls,but the difference was not statistically significant(P＞0.05).(2)The frequency of tit genotype or T allele in the moderate to severe AD patients was significantly higher than that in mild AD patients(25.8%vs.12.5%,χ2=5.477,P＜0.05;51.9%vs.40.3%,χ2=5.803,P＜0.05 respectively).(3)MMSE scores of the AD patients with TT genotype was significantly lower than ones with CC or CT genotype(P=0.028;P=0.014 respectively).Conclusion The 2616C/T polymorphism in the IRP2 gene is possibly associated with moderate to severe AD.but not associated with VD.And the TT genotype may be a risk factor for cognitive impairment of patients with AD in Chinese Han.     

铁调节蛋白2基因多态性与阿尔茨海默病及血管性痴呆的相关分析

Objective To evaluate the association of 2616c/T polymorphism in iron regulatory protein 2(IRP2)gene with Alzheimer disease(AD)and Vascular dementia(VD).Methods In this study,281 patients with AD,60 with VD,and 285 normal aged were recruited.The 2616C/T polymorphism in IRP2 gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism.And the cognitive function was assessed with the Mini-Mental State Examination(MMSE).Results (1)No significant difieFences were demonstrated in IRP2 genotype or allele frequencies between AD patients and controls(χ2=2.46,P=0.292;χ2=2.17,P=0.141 respectively).However,when AD patients were stratified by disease severity.the frequency of T allele carriers in the moderate to severe AD patients was 78.0%,significantly higher than that in controls(69.8%;χ2=4.106,P＜0.05).Logistic regression analysis demonstrated that the age-,sex-and ApoE-adiusted OR of modcrate to severe AD patient with T allele was 1.62(95% CI=1.03-2.54).The frequency of T allele carriers or T allele in VD patients was higher than that of controls,but the difference was not statistically significant(P＞0.05).(2)The frequency of tit genotype or T allele in the moderate to severe AD patients was significantly higher than that in mild AD patients(25.8%vs.12.5%,χ2=5.477,P＜0.05;51.9%vs.40.3%,χ2=5.803,P＜0.05 respectively).(3)MMSE scores of the AD patients with TT genotype was significantly lower than ones with CC or CT genotype(P=0.028;P=0.014 respectively).Conclusion The 2616C/T polymorphism in the IRP2 gene is possibly associated with moderate to severe AD.but not associated with VD.And the TT genotype may be a risk factor for cognitive impairment of patients with AD in Chinese Han.     

关于《中国药典》2000年版(一部)含朱砂(兼含雄黄)成方制剂问题的讨论

国际上对药物的重金属含量有严格限制,中药因重金属超标在出口外销时遇到抵制众所周知。然而,《中国药典》收载含朱砂(主成分硫化汞含量超过90 % )成方制剂品种数量却逐渐增加,到2 0 0 0年版达45个之多。本文试就这45个含朱砂成方制剂的有关问题进行讨论,旨在抛砖引玉,引起广泛重视,并为新版药典的修订提供参考。1　含朱砂成方制剂概况拟讨论的有关问题的主要资料和建议见表1。2　讨论与建议2 1　45种含朱砂的成方制剂是否都需....     

从制备方法看中药注射剂

常用中药剂型的制法,当属注射剂技术要求最高、研制难度最大、存在问题最多。笔者针对中药注射剂的药物组成已发表了文章[1],现进一步讨论其制法的相关问题。1中药注射剂制法概况笔者仅从1985年我国《药品管理法》颁布执行以来,经国家正式批准纳入《卫生部药品标准·中药成方制剂》(第1~20册)、国家药品监督管理局《国家中成药标准汇编》(13册)、《新药转正标准》(1~44册)和《中国药典》2005年版一部收载的目前仍在生产使用的10....     

对编写《中国药典》2005年版一部的建议(一)

在“突出特色,立足提高”原则指导下编写的《中国药典》2 0 0 0年版一部(简称《中国药典》) ,确实于突出中医药特色,大幅度提高中药现代化水平方面取得长足进展,可喜可贺。作为代表国家水平且具法典性质的药典,其编写是项复杂的系统工程,特别是国外尚无同类药典可资借鉴,操作起来难度更大。为使药典日臻完善,本文从临床角度提若干意见,供编写新版药典参考。1　关于功能与主治对药物功能与主治的表述,是极具鲜明中医药学特色的内....     

降低中药制剂含糖量的意义、思路和方法

中华人民共和国卫生部、国家医药管理局曾联合发出“关于抓紧研究降低部分药品含糖比例的通知”[(88)卫药字第29号]。为此,本刊组织了降低中药制剂中含糖量的专题稿件,分两期刊出,以期推动该项工作的进行。     

抑郁症与血管紧张素转化酶基因多态性的关联研究

目的 探讨单相抑郁症、双相抑郁症患者血管紧张素转化酶(ACE)基因第16内含子中287bp插入/缺失(I/D)多态的分布,以及ACE基因多态与抑郁症的关联性.方法 以100例抑郁症患者和100例正常对照组为对象进行病例对照研究,采用PCR检测ACE基因I/D多态性.结果 该多态位点的基因型在抑郁症组和正常对照组之间总体分布差异无显著性(P＞0.05),两组间等位基因差异无显著性(P＞0.05).结论 ACE基因I/D多态性与抑郁症不存在关联.     

辨证治疗肾病综合征顽固性水肿60例临床观察

以温阳利水法为主治疗６０例，浮肿消退占５０％，部分消退占４１．７２％，无变化占８．３％；治疗前后２４ｈ尿蛋白定量、血浆白蛋白、总胆固醇无变化；血尿素氮较治疗前差别显著；前列腺素Ｉ２治疗后较治疗前提高，差别显著；血栓素Ａ２治疗后较治疗前降低，差别显著。     

DiaSys FE-2粪便分析工作站的临床应用

目的评价DiaSys FE-2粪便分析工作站的临床应用价值。方法对503份粪便标本用直接涂片镜检法、加藤氏玻璃纸厚层涂片法、FE-2工作站法进行肠道寄生虫检查。结果 FE-2工作站法较直接涂片法虫卵检出率提高5.55倍,较加藤氏玻璃纸厚层涂片法虫卵检出率提高4.87倍;对蛔虫卵检出率提高10倍、鞭虫卵、钩虫卵检出率提高5倍,华支睾吸虫卵检出率提高4.5倍。结论 FE-2工作站设计新颖、标准定量、准确可靠。操作简便、卫生环保,能有效提高肠道寄生虫检出率,是一台适用于肠道寄生虫病防治的实用性仪器,值得推广使用。     

黄芪多糖对地塞米松诱导的脾淋巴细胞凋亡的影响

目的研究黄芪多糖对地塞米松诱导的脾淋巴细胞凋亡的影响.方法采用体外细胞培养的方法,以荧光显微镜、电镜、琼脂糖凝胶电泳、流式细胞仪分析等技术进行观测.结果荧光显微镜、电镜、琼脂糖凝胶电泳显示地塞米松(10-6mol/L)处理组出现典型的细胞凋亡表现,黄芪多糖处理组较地塞米松组有一定程度的恢复;流式细胞仪分析显示黄芪多糖处理组与地塞米松组相比,脾淋巴细胞凋亡率减少(P＜0.05).结论黄芪多糖对地塞米松诱导的脾淋巴细胞凋亡具有一定的抑制作用.