大鼠视前内侧区的传出性神经纤维联系—WGA—HRP法研究

应用WGA-HRP顺行轴突运输研究大鼠视前内侧区传出性神经纤维投射。结果表明:视前内侧区的上行投射向嘴侧经斜角带进入外侧隔核;经髓纹进入缰核;经无名质进入杏仁前区及经终纹进入杏仁内侧核,另有标记纤维经内侧前脑束向外下行,经视束上方进入杏仁内侧核。下行投射经内侧前脑束进入下丘脑室旁核、外侧区、内侧核、后核、弓状核、乳头体前腹核和乳头体上核。继续向尾侧,标记纤维进入中脑腹侧背盖区,并投射到中缝正中核及中缝背核。     

穴位注射加中药治疗颈性眩晕180例

目的观察穴位注射加中药治疗颈性眩晕的临床疗效。方法选用香丹注射液4ml加2％利多卡因2ml,在风池穴注射,同时口服滋补肝肾中药。结果经治疗3个疗程后,总有效率达95％。结论此法疗效满意,且简便、安全、价廉,值得推广。     

脑胶质瘤浸润性淋巴细胞的研究进展（综述）

应用免疫活性淋巴细胞作过继性免疫治疗(adop-tive immunotherapy)为临床治疗脑胶质瘤开辟了一条崭新的途径。继对 LAK 细胞(lymphokine-activatedkiller)的研究和临床试用性治疗脑胶质瘤之后,又发展到对第二代抗肿瘤效应细胞-TIL(tumor-infiltratinglymphocytes)的研究。随着免疫生物学的发展和免疫     

过继性免疫治疗脑胶质瘤的实验研究

本文报告30例脑胶质瘤病人淋巴因子激活的杀伤细胞(LAK)抗肿瘤活性。杀伤 K562细胞为46.0%±17.0%,Daudi 细胞为42.2%±14.2%,自体胶质瘤细胞为40.9%±15.9%,异体胶质瘤细胞为38.7±10.8%,不杀伤自体淋巴细胞。与健康对照组相比,病人 LAK 细胞具有同等的抗肿瘤活性(p>0.05);病人 NK 活性低于对照组(P<0.05)。     

Biocharacterizations of T lymphocytes infiltrating human primary brain gliomas

Glioma-infiltrating lymphocytes(GIL)were isolated from 9 surgical biopsy specimens of pri-mary brain gliomas using mechanical and enzymatic digestion and discontinuous density gradientcentfifugation.Durng culture in the presence of interleukin-2(IL-2)for a period of four weeks,GIL were expanded by 48.4-fold on the avea-age,even up to 118-fold.GIL activated by IL-2 hadspcific cytolytic activity against autologous glioma cells.Analysis of cell surface phenotypes offreshly isolated GIL showed that CD3~+ cells were 71.0±11.9％,CD4~+ cells 34.2±6.1％ and CD8~+cells 37.0±7.6％.Ability of IL-2-activated GIL to secrete γ-interferon(γ-IFN)was significantlyhigher than that of freshly isolated GIL and autologous peripheral blood lymphocytes(PBL).Theresults suggest that GIL have many advantages as an adoptive immunotherapy for patients withgliomas and as a new type of antitumor immune effector.     

胆结石的中药治疗近况

自第二次世界大战以来,人类的胆结石的发生率几乎以每10年增长2倍的速度急剧上升.在某些发达国家中,胆结石并发胆囊疾病者已占住院病种的第五位,胆囊切除术,已成为仅次于阑尾切除术的常见手术.我国胆结石、胆囊炎的发病率也逐年上升,因此,胆结石症已成为医学上十分热门的研究课题.     

T SUBSETS AND ANTITUMOR ACTIVITY OF LYMPHOCYTES INFILTRATING HUMAN PRIMARY BRAIN GLIOMAS

Glioma-infiltrating lymphocytes (GIL) were isolated from 9 surgical biopsy specimens of primary brain gliomas using mechanical and enzymatic digestion and discontinuous density gradent centrifugation. During cultured in the presence of interleukin-2 (IL-2) for a period of four weeks, GIL were expanded 48. 4-fold on the average, even up to 118-fold. GIL activated by IL- 2 had specific cytolytic activity against autologous glioma cells. Analysis of T subsets of GIL freshly isolated showed that CD3 cells were 71.0±11.9%, CD4 cells 34.2±6.1% and CD3 cells 37.0±7.6%. Ability of activated GIL to produce γ-Interferon (γ-IFN) was significantly higher than that of freshly isolated GIL and autologous peripheral blood lymphocytes (PBL). The results suggest that GIL have many advantages for an adoptive immunotherapy of patients with brain gllomas and be a new type of antitumor immune effector.     

脑胶质瘤浸润淋巴细胞的生物学特性

本文应用酶消化和不连续密度梯度离心方法,从9例原发性脑胶质瘤中分离出肢质瘤浸润淋巴细胞(GIL),并对其生物学特性进行分析,结果为:GIL体外长期培养生长能力强于LAK细胞;GIL的NK活性和产生γ-干扰素能力低下,经IL-2培养激活后,NK活性和产生γ-干扰素能力显著提高;GIL中71.0±11.9痞为CD_8~+的T淋巴细胞,其中CD_8~+细胞多于CD_4~+细胞。结果表明浸润在胶质瘤内的淋巴细胞功能低下,缺乏直接和间接杀伤肿瘤细胞活性。经IL-2激活后,GIL的抗肿瘤活性显著增强,可作为免疫治疗肿瘤的效应细胞。     

脑胶质瘤浸润淋巴细胞体外抗肿瘤活性及产生IL—2水平

本文动态地观察了9例经 IL-2活化的胶质瘤浸润淋巴细胞(GIL)体外抗肿瘤活性。结果表明 GIL 具有选择杀伤自体胶质瘤细胞特异性,同时也表达 NK 样活性和 LAK 样活性;而对自体淋巴细胞无破坏作用。GIL 产生 IL-2和 TNF 显著高于自体淋巴细胞和正常对照淋巴细胞。研究结果表明 IL—2活化的GIL 可能是较理想的过继性免疫治疗恶性胶质瘤的效应细胞。