徒手旋转胎头术对头位难产产妇分娩指标及新生儿神经行为的影响

目的 研究徒手旋转胎头术对头位难产产妇分娩指标及新生儿神经行为的影响。方法 将2018年2月—2020年1月在本院分娩的头位难产产妇87例纳入研究对象。依照电脑随机数字表法将研究对象分作研究组43例和对照组44例。对照组采取常规助产分娩,研究组在对照组护理的基础上采取徒手旋转胎头术助产分娩。分析并比较两组助产结局、分娩指标、产妇并发症发生率、新生儿并发症发生率以及新生儿神经行为的差异。结果 干预后,研究组的助产成功率为97.67%,高于对照组的81.82%(P<0.05);第二产程时间及总分娩时间均短于对照组(均P<0.05);两组产妇并发症发生率对比,差异无统计学意义(P>0.05);研究组新生儿并发症总发生率为9.30%,低于对照组的29.55%(P<0.05);研究组新生儿NBNA评分高于对照组(P<0.05)。结论 徒手旋转胎头术可明显改善头位难产产妇的妊娠结局,有助于保障母婴生命健康安全,值得临床推广应用。     

两种脱细胞方法制备组织工程气管基质的比较

BACKGROUND: Acellular tracheal matrix is similar to the native trachea with structure and biological performance preserved after decellularization, and it is an important aim in tissue engineering to find an effective method of decellularization. OBJECTIVE: To select the optimal decellularization method through comparing chemical extraction method with detergent-enzymatic method for preparing rabbit tissue engineering trachea matrix. METHODS: Thirty tracheas from New Zealand white rabbits were randomly divide into three groups. Twenty of rabbit tracheas were subjected to decellularization using 2% TritonX-100 combined with deoxyribonuclease I and ribonuclease (chemical extraction method group), and sodium deoxycholate combined with deoxyribonuclease I (detergent-enzymatic group), respectively. The other ten were given no intervention as controls. Samples were collected and observed by hematoxylin-eosin staining, Masson-trichrome staining, safranin O staining, DAPI staining and scanning electronic microscope. RESULTS AND CONCLUSION: Hematoxylin-eosin staining demonstrated that compared with the control group, almost all cellular components of the mucosal epithelium were removed in the detergent-enzymatic and chemical extraction groups, and there were few remnant chondrocytes. Masson-trichrome staining indicated that compared with the control group, components of the mucosal layer chondrocytes in the chemical extraction and detergent-enzymatic groups were completely removed, with only part of remained chondrocytes in the cartilage zone. Glycosaminoglycan was slightly decreased both in the chemical extraction and detergent-enzymatic groups shown by Safranin O staining, but more reduction was found in the chemical extraction group. DAPI staining reveled that only a small amount of cartilage cells remained in the dense layer of cartilage and lacuna both in this two methods. Scanning electronic microscope showed that using the detergent-enzymatic method there were the hierarchical structures of native trachea, but slight disruption using the chemical extraction method. In conclusion, decellularized rabbit trachea matrix obtained by detergent-enzymatic method is better, with little disruption to the matrix. 中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程     

短暂性完全遗忘综合征22例报告

目的：对22例短暂性完全遗忘综合征病人进行临床观察，并探讨其发病机理。方法：我们根据Caplan诊断标准诊断了22例TGA病人，行多普勒，头CT、MRI、MRA，脑电图，血流变等检查，分析了检查结果，查找TGA病因。结果：22例患均行多普勒检查，18例示椎－脊底动脉供血不足，4例正常。头CT检查，11例有腔隙性脑梗死，3例脑萎缩，8例未见异常。其中6人同时进行头MRI检查，其中3人腔隙性脑梗死，3例正常。20例脑电图检查，4例异常，血液流变学检测，均有不同程度血液流变学改变。结论：TGA经治疗预后良好。