全文获取类型
收费全文 | 5355篇 |
免费 | 294篇 |
国内免费 | 28篇 |
专业分类
耳鼻咽喉 | 68篇 |
儿科学 | 95篇 |
妇产科学 | 92篇 |
基础医学 | 809篇 |
口腔科学 | 78篇 |
临床医学 | 398篇 |
内科学 | 1197篇 |
皮肤病学 | 97篇 |
神经病学 | 572篇 |
特种医学 | 186篇 |
外科学 | 658篇 |
综合类 | 52篇 |
一般理论 | 4篇 |
预防医学 | 311篇 |
眼科学 | 79篇 |
药学 | 498篇 |
中国医学 | 35篇 |
肿瘤学 | 448篇 |
出版年
2023年 | 33篇 |
2022年 | 79篇 |
2021年 | 220篇 |
2020年 | 83篇 |
2019年 | 126篇 |
2018年 | 145篇 |
2017年 | 103篇 |
2016年 | 121篇 |
2015年 | 137篇 |
2014年 | 176篇 |
2013年 | 261篇 |
2012年 | 431篇 |
2011年 | 401篇 |
2010年 | 234篇 |
2009年 | 200篇 |
2008年 | 366篇 |
2007年 | 343篇 |
2006年 | 341篇 |
2005年 | 328篇 |
2004年 | 317篇 |
2003年 | 257篇 |
2002年 | 272篇 |
2001年 | 53篇 |
2000年 | 42篇 |
1999年 | 37篇 |
1998年 | 44篇 |
1997年 | 34篇 |
1996年 | 44篇 |
1995年 | 32篇 |
1994年 | 31篇 |
1993年 | 25篇 |
1992年 | 23篇 |
1991年 | 28篇 |
1990年 | 19篇 |
1989年 | 13篇 |
1988年 | 16篇 |
1987年 | 12篇 |
1986年 | 15篇 |
1985年 | 13篇 |
1984年 | 22篇 |
1983年 | 17篇 |
1982年 | 15篇 |
1981年 | 18篇 |
1980年 | 11篇 |
1979年 | 17篇 |
1978年 | 12篇 |
1977年 | 17篇 |
1973年 | 14篇 |
1972年 | 9篇 |
1968年 | 9篇 |
排序方式: 共有5677条查询结果,搜索用时 328 毫秒
1.
2.
3.
4.
5.
Gary D. Pipes Phillip Campbell Pavel V. Bondarenko Bruce A. Kerwin Michael J. Treuheit Himanshu S. Gadgil 《Journal of pharmaceutical sciences》2010,99(11):4469-4476
A middle-down LC/MS approach, for the rapid quantitation and characterization of site-specific methionine oxidation in a recombinant monoclonal IgG1 molecule, is described. An IgG1 antibody was digested with endoprotease LysC under limited proteolytic conditions to produce two major components; an antigen binding fragment (Fab) and a crystallizable fraction (Fc). These fractions were then reduced to produce three major species; light chain (LC), Fc/2 which is the C terminal region of the heavy chain (HC) and the N-terminal heavy chain region (Fd). These three fragments were separated by reversed-phase HPLC using a diphenyl column. The diphenyl column resolved site-specific methionine oxidation in all three subunits. Middle- down N-terminal sequencing with a LCT premier mass spectrometer was used to identify the sites of oxidation in the LC. Sites of oxidation in the Fc/2 were identified using middle-down collision-induced dissociation (CID) on a Qtof premier. This method allowed for the rapid quantitation and identification of oxidation on each methionine residue in an IgG1 molecule. 相似文献
6.
A tumor-targeted and conditionally replicating oncolytic adenovirus vector expressing TRAIL for treatment of liver metastases. 总被引:3,自引:0,他引:3
Pavel Sova Xiao-Wei Ren Shaoheng Ni Kathrin M Bernt Jie Mi Nancy Kiviat André Lieber 《Molecular therapy》2004,9(4):496-509
We have constructed a new capsid-modified adenovirus (Ad) vector that specifically replicates in tumor cells and expresses TNF-related apoptosis-inducing ligand (TRAIL). The Ad capsid contains short-shafted fibers derived from Ad serotype 35, which allow for efficient infection of malignant tumor cells, and largely avoids innate toxicity after intravenous application. Replication-dependent homologous recombination in Ad genomes was used to achieve tumor-specific expression of Ad E1a (to mediate viral replication) and TRAIL (to mediate apoptosis and enhance release of progeny virus from infected cells). We demonstrated that our oncolytic vector (Ad5/35.IR-E1A/TRAIL) induced apoptosis in human tumor cell lines derived from colorectal, lung, prostate, and liver cancer. Both in vitro and in vivo tumor models showed efficient intratumoral spread of this vector. In a model for metastatic colon cancer, tail vein infusion of Ad5/35.IR-E1A/TRAIL resulted in elimination of preestablished liver metastases. Intravenous injection of this vector caused a transient elevation of serum glutamic pyruvic transaminase in tumor-bearing mice, which we attributed to factors released from apoptotic tumor cells. Liver histology analyzed at day 14 after virus injection did not show signs of hepatocellular damage. This new oncolytic vector represents a potentially efficient means for gene therapy of metastatic cancer. 相似文献
7.
Stem cells and periodontal regeneration 总被引:10,自引:0,他引:10
8.
Various neocortical areas from four females aged 16–24 years with Rett syndrome (RS) were investigated and compared with
brains of therapy-resistant partial epilepsy (TRPE) patients (18–25 years), infantile autism (IA), and control brains (24
and 58 years). The cytoarchitecture of area 10 (frontal), area 21 (temporal), area 4 (primary motor cortex), and area 17 (primary
visual cortex) was studied by the combined Klüver-Barrera (luxol fast blue and cresyl violet) standard procedure. Autofluorescence
of lipofuscin, immunofluorescence of synaptic vesicle proteins [synaptophysin (p38)] and lectin-stained (Wisteria floribunda agglutinin) perineuronal nets (PNs) were studied in the cortices using dual-channel confocal laser scanning microscopy. The
brains of RS females show various types of morphological/cytoarchitectonical abnormalities of single pyramidal neurons in
layers II–III, and V–VII of different cortical areas. The abnormalities include mild losses of pyramidal neurons, more pronounced
in layers II and III than in layers V and VII, and more evident in frontal and temporal areas than in the visual cortex. Microdysgenesis,
including abnormalities due to neuronal migration disorders, was not found in RS, in contrast to the observations in TRPE
patients, strongly indicating that RS is not a neuronal migration disorder. Lipofuscin distribution was normal but amounts
were lower in RS cases than in control and TRPE brains. PNs were less expressed in cortices of the IA case, but were clearly
overexpressed in the motor cortex of RS. Quantitative analysis of p38 showed a decrease in the area occupied by p38 immunoreactivity
by 20–40% in RS compared with controls. It is concluded that RS could best be explained by a postnatal synaptogenic developmental
deficiency; the basic defect, however, is still completely unknown.
Received: 26 February 1996 / Revised, accepted: 11 July 1996 相似文献
9.
Iwar Klime Antonian Vraana Jaroslav Kune Elena eboUkovaA Zdena Dobe ovaa Pavel tolba Josef Zicha 《Blood pressure》1995,4(3):137-142
Hereditary hypertriglyceridemic rats (hHTg) were developed as a new genetic model for the study of relationships between blood pressure (BP) and metabolic abnormalities. This strain has been produced by selective inbreeding from Wistar rats according to the rise of plasma triglycerides induced by a high-sucrose diet. Though hHTg rats display hypertriglyceridemia, impaired glucose tolerrance, hyperinsulinemia, insulin resistance and increased BP even without nutritional stimuli, high sucrose feeding further aggravates these symptoms. High plasma triglycerides levels in hHTg rats seem to be a consequence of their hyperproduction. Impaired insulin action is responsible for the defective glucoregulation in this strain. The loss of insulin responsiveness might be due to a reduction in the number of glucose transporters. Highly significant relationships among plasma triglycerides, ouabain-resistant Na+ transport and BP were demonstrated in the hHTg rats. Segregating populations (F2 hybrids) should be used for genetic analysis of the primary role of lipid and/or ion transport abnormalities in the pathogenesis of this form of genetic hypertension. 相似文献
10.
Automatic assessment of levodopa-induced dyskinesias in daily life by neural networks. 总被引:1,自引:0,他引:1
We developed an objective and automatic procedure to assess the severity of levodopa-induced dyskinesia (LID) in patients with Parkinson's disease during daily life activities. Thirteen patients were continuously monitored in a home-like situation for a period of approximately 2.5 hours. During this time period, the patients performed approximately 35 functional daily life activities. Behavior of the patients was measured using triaxial accelerometers, which were placed at six different positions on the body. A neural network was trained to assess the severity of LID using various variables of the accelerometer signals. Neural network scores were compared with the assessment by physicians, who evaluated the continuously videotaped behavior of the patients off-line. The neural network correctly classified dyskinesia or the absence of dyskinesia in 15-minute intervals in 93.7, 99.7, and 97.0% for the arm, trunk, and leg, respectively. In the few cases of misclassification, the rating by the neural network was in the class next to that indicated by the physicians using the AIMS score (scale 0-4). Analysis of the neural networks revealed several new variables, which are relevant for assessing the severity of LID. The results indicate that the neural network can accurately assess the severity of LID and could distinguish LID from voluntary movements in daily life situations. 相似文献