Malignant Warthin''s tumour: a case study

Malignant transformation of a benign Warthin's tumour (adenolymphoma) is extremely rare. The light microscopic and ultrastructural features of an adenocarcinoma arising in a Warthin's tumour in the parotid gland are described. Light microscopy demonstrated a transition zone from the benign to the malignant component, and the malignant cells revealed oncocytic features by both light- and electronmicroscopy.     

Immunocytochemical demonstratin of c-fos protein in sertoli cells and germ cells in rat testis

The juxtaglomerular apparatus fulfils several important regulatory functions in the kidney, such as tubuloglomerular feedback (TGF) control and control of renin release. The macula densa (MD) cells sense the fluid load by perceiving the distal NaCl concentration via a Na-K-2Cl cotransport system in the luminal cell membrane. It has been proposed that macula densa cell activation may involve changes in intracellular cytosolic free calcium concentration ([Ca²⁺]₁), as one link in the chain of events activating TGF or releasing renin. We therefore investigated the changes in the intracellular calcium concentrations with fura-2, using a video system, in macula densa cells, and compared them with the changes in the corresponding concentrations in the ascending limb of the loop of Henle (c-TAL). The results show that our technique for analysing intracellular cystolic free calcium in isolated perfused tubules is valid for this purpose, and the K_d value obtained was similar to that found by Grynkiewicz et al. (1985). The intracellular cystolic free calcium concentration was about 90 nM both in the macula densa and c-TAL cells, and the macula densa cell intracellular cystolic free calcium concentration increased by about 20 nM when the tubular lumen was perfused with Na and C1 at low concentrations. No significant changes were noted when furosemide was added to the perfusion solutions. We consider it hardly likely that this small change in intracellular cystolic free calcium concentration can be entirely responsible for full activation of renin release or full inactivation of the TGF control mechanism. It would seem that the signal transmission from the macula densa cells could occur by other routes than through activation of intracellular cytosolic free calcium concentration.     

Identification of αβ and γδ T Cell Receptor-Positive Cells

Two lineages of T lymphocytes bearing the CD3 antigen can be defined on the basis of the nature of the heterodimeric receptor chain (alpha beta or gamma delta T cell receptor (TCR) expressed. Precise identification of alpha beta and gamma delta TCR+ cells is essential when studying the tissue distribution and function of these different T cells. In immunofluorescence studies gamma delta TCR+ cells have been identified as CD3+WT-31- or CD3+CD4-CD8- cells. However, this may not be the optimal procedure because gamma delta TCR+ cells are weakly WT-31+, and some are CD8+. The aim of this study was to evaluate a panel of monoclonal antibodies (MoAb) directed against different chains of the TCR-T3 complex for a more precise identification of alpha beta+ and gamma delta TCR+ cells in flow cytometric studies. We found that the MoAb anti-Ti-gamma A and delta-TCS-1, recognizing the TCR-gamma and the TCR-delta chain respectively, only reacted with a subpopulation of gamma delta TCR+ cells, whereas another TCR-delta chain recognizing MoAb anti-TCR-delta 1 reacted with all gamma delta TCR+ cells. All MoAb reported to belong to the CD3 group reacted with both alpha beta TCR+ and gamma delta TCR+ cells as expected. Our results indicate that all gamma delta TCR+ cells can be identified with the MoAb anti-TCR-delta 1. Because no MoAb recognizing the TCR-alpha or TCR-beta chains at the cell surface of intact cells are yet available, we suggest that alpha beta TCR+ cells could be identified as CD3+ anti-TCR-delta 1-cells.     

COMPARISON OF SUFENTANIL-NITROUS OXIDE ANAESTHESIA WITH FENTANYL-NITROUS OXIDE ANAESTHESIA IN GERIATRIC PATIENTS UNDERGOING MAJOR ABDOMINAL SURGERY

We have measured haemodynamic changes and plasma concentrationsof catecholamines during sufentanil-nitrous oxide and fentanyl-nitrousoxide anaesthesia in a controlled, randomized, double-blindstudy of 20 geriatric patients (age 65–86 yr) undergoingmajor abdominal surgery. Fentanyl 7 µg kg^–1 followedby infusion of 3 µg kg^–1 h^–1 was comparedwith sufentanil 1 µg kg^–1 followed by 0.4 µgkg^–1 h^–1. The opioid was supplemented with 60–67%nitrous oxide in oxygen. Haemodynamic changes, plasma concentrationsof catecholamines (by high pressure liquid chromatography) andopioids (by radio-immunoassay), and myocardial lactate extractionwere measured in the awake state, and at defined times duringanaesthesia and surgery. Haemodynamic state was stable duringinduction and trachea/ intubation in both groups, while duringstressful operative periods there were increases in mean arterialpressure (17%in the fentanyl group; 11% in the sufentanil group),heart rate (fentanyl 20%, sufentanil 14%) and plasma concationsof catecholamines (adrenaline: fentanyl 316%, sufentanil 86%;noradrenaline: fentanyl 78%, sufentanil 186%) in both groups.Sufentanil was similar to fentanyl in attenuating the haemodynamicand hormonal responses to surgical stimulation. In two patientsin the fentanyl group and three in the sufentanil group, myocardiallactate production was observed temporarily, indicating myocardialischaemia caused by surgical stress. ^*Present address: Department of Anaesthesiology, Universityof Homburg, Oscar-Orth-Straße D-6650 Homburg/Saar, Germany Results presented in pan at the 11th Annual Meeting of the EuropeanAcademy of Anaesthesiologists, Bonn, August 31, 1989     

Peripherally inserted central catheters in infants and children – indications,techniques, complications and clinical recommendations

Venous access required both for blood sampling and for the delivery of medicines and nutrition is an integral element in the care of sick infants and children. Peripherally inserted central catheters (PICCs) have been shown to be a valuable alternative to traditional central venous devices in adults and neonates. However, the evidence may not extrapolate directly to older paediatric patients. In this study, we therefore review the indications, methods of insertion and complications of PICC lines for children beyond the neonatal age to provide clinical recommendations based on a search of the current literature. Although the literature is heterogeneous with few randomised studies, PICCs emerge as a safe and valuable option for intermediate‐ to long‐term central venous access in children both in and out of hospital. Insertion can often be performed in light or no sedation, with little risk of perioperative complications. Assisted visualisation, preferably with ultrasound, yields high rates of insertion success. With good catheter care, rates of mechanical, infectious and thrombotic complications are low and compare favourably with those of traditional central venous catheters. Even in the case of occlusion or infection, fibrinolytics and antibiotic locks often allow the catheter to be retained.