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排序方式: 共有142条查询结果,搜索用时 125 毫秒
1.
Patrizia Noris Eloisa Arbustini Pierangelo Spedini Simona Belletti & Carlo Luigi Balduini 《British journal of haematology》1998,103(4):1004-1013
We describe a new variant of Bernard-Soulier syndrome characterized by almost normal amounts of GPIb and severely reduced GPIX and GPV. Despite surface expression, GPIbα failed to support ristocetin-induced platelet agglutination and to bind two conformation-dependent monoclonal antibodies, suggesting a qualitative defect. Sequence analysis of the gene coding for GPIX revealed a T-to-C substitution at base 1811, leading to a Leu40Pro conversion, whereas no defects were found in the coding region of the GPIbα gene. Allele-specific restriction enzyme analysis showed that the propositus and one of his sisters, both with severe bleeding diathesis, were homozygous for the GPIX mutation; the members of the family with mild bleeding diathesis and/or giant platelets in the peripheral blood were heterozygous, whereas the healthy ones were homozygous for the normal allele.
Infusion of 1-desamino-8- d -arginine vasopressin normalized bleeding time in the two severely affected patients, although it did not modify ristocetin-induced platelet agglutination or membrane expression of GPIbα, GPIX, GPIIb–IIIa and GMP-140. Moreover, in one patient, normalization of bleeding time and rise of von Willebrand factor plasma concentration did not seem to be directly related. 相似文献
Infusion of 1-desamino-8- d -arginine vasopressin normalized bleeding time in the two severely affected patients, although it did not modify ristocetin-induced platelet agglutination or membrane expression of GPIbα, GPIX, GPIIb–IIIa and GMP-140. Moreover, in one patient, normalization of bleeding time and rise of von Willebrand factor plasma concentration did not seem to be directly related. 相似文献
2.
3.
Pedrazzoli P Noris P Perotti C Schiavo R Ponchio L Belletti S Da Prada GA Balduini CL Salvaneschi L Robustelli Della Cuna G Siena S 《British journal of haematology》2000,108(3):653-659
We have recently reported the possibility of supporting the phase of severe thrombocytopenia after high-dose chemotherapy (HDC) and stem cell transplantation using 5% dimethylsulphoxide (DMSO)-cryopreserved autologous platelet concentrates (PCs). The aim of the present study was to evaluate the therapeutic potential of ThromboSol (a recently developed platelet storage solution) plus PCs cryopreserved in 2% DMSO in patients undergoing myeloablative chemotherapy and autologous transplantation. PCs were collected from 14 women with breast cancer by a single plateletapheresis and cryopreserved in ThromboSol/2% DMSO by either direct insertion in a -80 degrees C freezer or in liquid nitrogen after computer-controlled rate (CR) freezing. When required, PCs were thawed, centrifuged to remove the cryoprotectants and transfused. In vitro studies on thawed platelets showed loss of epitopes of surface glycoproteins and a marked reduction of functional activity compared with fresh platelets. Transfusion of CR-frozen PCs was associated with a mean 1 h corrected count increment (CCI) of 9.2 +/- 5.4 x 109/l and only one allogeneic PC was required in this group. In contrast, six out of seven patients required additional allogeneic transfusions in the -80 degrees C group (CCI = 2.7 +/- 1.4 x 109/l). ThromboSol-treated PCs have the ability to overcome thrombocytopenia if processed by a CR freezing protocol, but appear ineffective when frozen by direct placing at -80 degrees C. 相似文献
4.
In previous articles we described how the Italian system of aid for people with disabilities also provides a system of incentives and subsidies for devices that are very useful,in addition to the provision of aids that are granted free of charge as required under an individual rehabilitation plan.We have also seen as aids for overcoming architectural barriers are (rightly!) in the first place among those who are essential for people with disabilities to lead an independent life and to be able to access public and private places,in particular workplaces.Without removing of architectural barriers it is impossible to achieve many of the rights decreed by the Italian law n.104 and n.68 (work integration of disabled people).Here you can find the incentives provided by Italian law and the features of the two most common systems:stairlifts and platform lifts. 相似文献
5.
Critical role of cyclin D3 in TSH-dependent growth of thyrocytes and in hyperproliferative diseases of the thyroid gland 总被引:4,自引:0,他引:4
Motti ML Boccia A Belletti B Bruni P Troncone G Cito L Monaco M Chiappetta G Baldassarre G Palombini L Fusco A Viglietto G 《Oncogene》2003,22(48):7576-7586
We report that cyclin D3 is rate limiting for G1 progression in thyroid follicular cells and that its constitutive upregulation by chronic stimulation of the TSH/cAMP pathway plays a role in human and experimental hyperproliferative diseases of the thyroid gland. These conclusions are supported by in vitro and in vivo studies. In rat thyrocytes (PC Cl 3 cells), cyclin D3 expression is enhanced in response to activation of the TSH/cAMP pathway. Interference with the expression of G1 cyclins (in particular cyclin D3) by the antisense methodology strongly reduced TSH-dependent proliferation of PC Cl 3 cells, indicating that proper progression through G1 requires cyclin D3. Accordingly, PC Cl 3 cells engineered to overexpress cyclin D3 (PC-D3 cells) show enhanced growth rate and elude hormone-dependence and contact inhibition. Using an animal experimental model of thyroid stimulation, we demonstrate that cyclin D3 is a key mediator of TSH-dependent proliferation of thyroid follicular cells also in vivo. Cyclin D3 protein levels were higher in the thyrocytes from glands of propylthiouracil-treated rats compared with control animals. The increase in cyclin D3 expression occurred after the propylthiouracil-induced increase in TSH levels and preceded the burst of cell proliferation. Finally, we found that cyclin D3 protein is expressed in a fraction of human goiters but it is strongly overexpressed in most follicular adenomas. 相似文献
6.
Sergio Belletti Gianni Gobbi Ugo Guerra 《International Journal of Clinical & Laboratory Research》1979,9(1):91-99
Summary Because of the growing interest in131Cs as a tracer for myocardium and thyroid scintigraphy, its internal dosimetry in man was reviewed and determined. The absorbed
dose was calculated using the method of Loevinger and Berman, with minor modifications, i.e. taking into account the different
activities of the body organs and their contribution to the absorbed dose. Special attention was also paid to the softer and
non-penetrating radiations, namely L and M, capture events and Auger electrons, in relation to the decay of the nuclide. Dosimetric
data for the thyroid were determined experimentally. 相似文献
7.
Alberto Zangrillo Gabriele Alvaro Alessandro Belletti Antonio Pisano Luca Brazzi Maria G. Calabrò Fabio Guarracino Tiziana Bove Evgeny V. Grigoryev Fabrizio Monaco Vladimir A. Boboshko Valery V. Likhvantsev Anna M. Scandroglio Gianluca Paternoster Rosalba Lembo Samuele Frassoni Marco Comis Vadim V. Pasyuga Vladimir V. Lomivorotov 《Journal of cardiothoracic and vascular anesthesia》2018,32(5):2152-2159
8.
Elena Bignami Alessandro Belletti Paola Moliterni Elena Frati Marcello Guarnieri Luigi Tritapepe 《Journal of clinical monitoring and computing》2016,30(3):347-365
This study was to investigate and define what is considered as a current clinical practice in hemodynamic monitoring and vasoactive medication use after cardiac surgery in Italy. A 33-item questionnaire was sent to all intensive care units (ICUs) admitting patients after cardiac surgery. 71 out of 92 identified centers (77.2 %) returned a completed questionnaire. Electrocardiogram, invasive blood pressure, central venous pressure, pulse oximetry, diuresis, body temperature and blood gas analysis were identified as routinely used hemodynamic monitoring, whereas advanced monitoring was performed with pulmonary artery catheter or echocardiography. Crystalloids were the fluids of choice for volume replacement (86.8 % of Centers). To guide volume management, central venous pressure (26.7 %) and invasive blood pressure (19.7 %) were the most frequently used parameters. Dobutamine was the first choice for treatment of left heart dysfunction (40 %) and epinephrine was the first choice for right heart dysfunction (26.8 %). Half of the Centers had an internal protocol for vasoactive drugs administration. Intra-aortic balloon pump and extra-corporeal membrane oxygenation were widely available among Cardiothoracic ICUs. Angiotensin-converting enzyme inhibitors were suspended in 28 % of the Centers. The survey shows what is considered as standard monitoring in Italian Cardiac ICUs. Standard, routinely used monitoring consists of ECG, SpO2, etCO2, invasive BP, CVP, diuresis, body temperature, and BGA. It also shows that there is large variability among the various Centers regarding hemodynamic monitoring of fluid therapy and inotropes administration. Further research is required to better standardize and define the indicators to improve the standards of intensive care after cardiac surgery among Italian cardiac ICUs. 相似文献
9.
Giovanni Merolla Arpit C. Dave Francesco Pegreffi Lorenza Belletti Giuseppe Porcellini 《Musculoskeletal surgery》2012,96(1):93-99
Alkaptonuria is a rare hereditary metabolic disease of autosomal recessive inheritance, resulting from deficiency of the enzyme homogentisic acid oxidase. The term “alkaptonuria” was first used in 1859 by Boedeker to describe a patient’s urinary reducing compound, and in 1866, Virchow coined the term “ochronosis” due to typical yellow pigmentation. Deposition of this pigment in articular cartilage leads to ochronotic arthropathy, the most incapacitating complication of alkaptonuria. We report a rare case of shoulder ochronotic arthritis, treated with total shoulder arthroplasty, achieving a successful long-term clinical and radiological outcomes. 相似文献
10.
Overexpressed cyclin D3 contributes to retaining the growth inhibitor p27 in the cytoplasm of thyroid tumor cells 总被引:9,自引:0,他引:9
Gustavo Baldassarre Barbara Belletti Paola Bruni Angelo Boccia Francesco Trapasso Francesca Pentimalli Maria Vittoria Barone Gennaro Chiappetta Maria Teresa Vento Stefania Spiezia Alfredo Fusco Giuseppe Viglietto 《The Journal of clinical investigation》1999,104(7):865-874
The majority of thyroid carcinomas maintain the expression of the cell growth suppressor p27, an inhibitor of cyclin-dependent kinase-2 (Cdk2). However, we find that 80% of p27-expressing tumors show an uncommon cytoplasmic localization of p27 protein, associated with high Cdk2 activity. To reproduce such a situation, a mutant p27 devoid of its COOH-terminal nuclear-localization signal was generated (p27-NLS). p27-NLS accumulates in the cytoplasm and fails to induce growth arrest in 2 different cell lines, indicating that cytoplasm-residing p27 is inactive as a growth inhibitor, presumably because it does not interact with nuclear Cdk2. Overexpression of cyclin D3 may account in part for p27 cytoplasmic localization. In thyroid tumors and cell lines, cyclin D3 expression was associated with cytoplasmic localization of p27. Moreover, expression of cyclin D3 in thyroid carcinoma cells induced cytoplasmic retention of cotransfected p27 and rescued p27-imposed growth arrest. Endogenous p27 also localized prevalently to the cytoplasm in normal thyrocytes engineered to stably overexpress cyclin D3 (PC-D3 cells). In these cells, cyclin D3 induced the formation of cytoplasmic p27-cyclin D3-Cdk complexes, which titrated p27 away from intranuclear complexes that contain cyclins A-E and Cdk2. Our results demonstrate a novel mechanism that may contribute to overcoming the p27 inhibitory threshold in transformed thyroid cells. 相似文献