8-甲氧补骨脂素和长波紫外线在细胞光老化中对端粒缩短机制的影响

Objective To investigate the mechanism of telomere shortening through 8-methoxypsoralen(8-MOP)and subsequent ultraviolet A(UVA)irradiation-induced photoaging model in human dermal fibroblasts(HDFs).Methotis Photoaging model was established by 8-MOP+UVA in skin HDFs.Flow cytometer.enzyme eytochemistry,immunofluorescence,Westem blot and Real-time PCR were employed.Results The percentage of G1 blockage of 8-MOP+UVA group were higher than that of control group at 24、48、72 h and 7 d(61.4%±1.5% vs 32.8%±1.5%.69.5%±2.2% vs 44.9% ±2.3%.88.2%±1.6% vs 59.8%±1.4%,90.7%±2.5% vs 68.5%±2.6%.all P＜0.01).The expression of SA-β-Gal of 8-MOP+UVA group were higher than that of control group at 24、48、72 h and 7 d(34.87%±0.59% vs 7.11%±0.78%,59.38%±0.46% vs 10.57%±0.47%.72.46%±0.98% vs 11.67%±0.87%,94.33%±0.13% vs 12.04%±0.12%,all P＜0.01).8-MOP+UVA treatment could significantly aggravate the oxidative DNA damages,the percentage of 8-oxo-dG positive cell of 8-MOP+UVA group(95.78%±0.14%)were significantly higher than that of control group(7.69%±0.09%,P＜0.01),8-MOP group(9.76%±0.11%,P＜0.01)and UVA group(35.29%±0.14%,P＜0.05).8-MOP+UVA treatment could accelerate the telomere shortening.the relative length of telomere of 8-MOP +UVA group were 2.57±0.05 lower than that of control group(6.63±0.12.P＜0.01).The levels of P53,P21WAF-1 and P16INK-4a of 8-MOP+UVA group were higher than that of control group(3.00±0.88 vs 0.54±0.10,2.50±0.51 vs 0.42±0.06,2.21±0.34 vs 0.38±0.05,all P＜0.01).Conclusion 8-MOP+UVA-induced photoaging of HDFs can be mediated though the regulation of telomere and subsequent P53-dependent signaling pathways.     

参芪扶正注射液延缓慢性肾衰竭临床与实验研究

目的：客观评价参芪扶正注射液治疗慢性肾功能衰竭（CRY）的疗效及安全性。方法：临床观察随机分组,治疗组（120例）给予参芪扶正注射液,对照组（60例）给予尿毒清;实验研究选取雌、雄性小鼠,随机分为正常对照组,模型组和参芪扶正注射液药物大、小剂量组,分别做负重游泳、灌胃急毒、动物模型治疗。结果：临床疗效治疗组明显优于对照组;实验动物无急性毒性反应,大剂量能显著提高小鼠抗疲劳能力及免疫功能,能较好地改善肾功能。结论：参芪扶正注射液无毒副作用,能维护和改善肾功能,从而延缓慢性肾功能衰竭的进程和延长病人的寿命。     

中医治疗289例乙型慢性活动性肝炎疗效观察

通过肝功能、血浆蛋白电泳、乙肝三系统、HBV—DNA—P等客观指标检测,对照观察了中医辨证治疗289例乙型慢性活动性肝炎的疗效,总有效率为87.53%,HBsAg转阴率为39.9%,HBeAg转阴率为68.5%,HBV—DNA—P转阴率为56.14%,各项指标明显优于对照组,统计学处理有非常显著性差异。     

兰索拉唑β-环糊精包合物的制备工艺研究

目的：制备兰索拉唑β-环糊精包合物。方法：用正交设计法研究物料比、温度、时间3个因素对兰索拉唑β-环糊精包合物制备工艺的影响。结果：包合温度对包封率有非常显著的影响,最佳工艺条件为兰索拉唑与β-环糊精的摩尔比为1：3,包合温度为30℃,超声时间为35min。结论：此方法可用于兰索拉唑β-环糊精包合物的制备。     

肿瘤病人抗生素使用情况的调查

1928年，美国微生物学家弗莱明发现了青霉素，并于20世纪40年代在美国研制成功，投入市场，从此进入了抗生素时代。半个多世纪以来，人类不断地研究、生产各种抗生素，而微生物（主要为细菌）也不断对抗生素产生耐药。肿瘤药人由于本身体质的原因，加上放疗、化疗以及免疫抑制剂的使用，极易出现由条件致病菌或耐药菌造成的院内感染，甚至出现院内感染暴发流行，严重威胁病人的健康和生命。为了使抗感染治疗获得最佳疗效。减少耐药菌株的产生。降低院内感染率，加强对抗生素的合理使用至关重要。按照卫生部《医院感染管理规范》的要求，我们对肿瘤病人抗生素的使用情况进行了摸底调查。     

EGCG对中波紫外线诱导HaCaT细胞p53基因和蛋白表达的实验研究

目的:研究表没食子儿茶素没食子酸酯(EGCG)对中波紫外线(UVB)照射诱导永生化角质形成细胞株-HaCaT细胞的p53 mRNA和p53蛋白表达的影响。方法:以一定剂量UVB照射HaCaT细胞,并以200μg/mL EGCG处理照射后的HaCaT细胞,分别用RT-PCR法和Western blot方法检测各处理条件下p53 mRNA和/或p53蛋白的表达水平。结果:30 mJ/cm2的UVB照射后HaCaT细胞的p53 mR-NA和p53蛋白表达逐渐增加,4 h达到峰值,4 h后随照射剂量增加而增加,24 h后有所恢复;加入EGCG可下调UVB诱导的表达作用。结论:UVB照射对HaCaT细胞p53 mRNA和p53蛋白的诱导表达有时效性与量效性,EGCG可下调UVB照射的这种诱导作用。     

8-甲氧补骨脂素和长波紫外线在细胞光老化中对端粒缩短机制的影响

Objective To investigate the mechanism of telomere shortening through 8-methoxypsoralen(8-MOP)and subsequent ultraviolet A(UVA)irradiation-induced photoaging model in human dermal fibroblasts(HDFs).Methotis Photoaging model was established by 8-MOP+UVA in skin HDFs.Flow cytometer.enzyme eytochemistry,immunofluorescence,Westem blot and Real-time PCR were employed.Results The percentage of G1 blockage of 8-MOP+UVA group were higher than that of control group at 24、48、72 h and 7 d(61.4%±1.5% vs 32.8%±1.5%.69.5%±2.2% vs 44.9% ±2.3%.88.2%±1.6% vs 59.8%±1.4%,90.7%±2.5% vs 68.5%±2.6%.all P＜0.01).The expression of SA-β-Gal of 8-MOP+UVA group were higher than that of control group at 24、48、72 h and 7 d(34.87%±0.59% vs 7.11%±0.78%,59.38%±0.46% vs 10.57%±0.47%.72.46%±0.98% vs 11.67%±0.87%,94.33%±0.13% vs 12.04%±0.12%,all P＜0.01).8-MOP+UVA treatment could significantly aggravate the oxidative DNA damages,the percentage of 8-oxo-dG positive cell of 8-MOP+UVA group(95.78%±0.14%)were significantly higher than that of control group(7.69%±0.09%,P＜0.01),8-MOP group(9.76%±0.11%,P＜0.01)and UVA group(35.29%±0.14%,P＜0.05).8-MOP+UVA treatment could accelerate the telomere shortening.the relative length of telomere of 8-MOP +UVA group were 2.57±0.05 lower than that of control group(6.63±0.12.P＜0.01).The levels of P53,P21WAF-1 and P16INK-4a of 8-MOP+UVA group were higher than that of control group(3.00±0.88 vs 0.54±0.10,2.50±0.51 vs 0.42±0.06,2.21±0.34 vs 0.38±0.05,all P＜0.01).Conclusion 8-MOP+UVA-induced photoaging of HDFs can be mediated though the regulation of telomere and subsequent P53-dependent signaling pathways.