全文获取类型
收费全文 | 39篇 |
免费 | 6篇 |
国内免费 | 1篇 |
专业分类
妇产科学 | 1篇 |
基础医学 | 3篇 |
临床医学 | 5篇 |
内科学 | 16篇 |
皮肤病学 | 1篇 |
神经病学 | 4篇 |
特种医学 | 1篇 |
外科学 | 2篇 |
预防医学 | 4篇 |
眼科学 | 1篇 |
药学 | 3篇 |
肿瘤学 | 5篇 |
出版年
2022年 | 1篇 |
2021年 | 1篇 |
2019年 | 3篇 |
2018年 | 1篇 |
2017年 | 1篇 |
2016年 | 1篇 |
2015年 | 2篇 |
2014年 | 3篇 |
2013年 | 2篇 |
2012年 | 4篇 |
2011年 | 4篇 |
2010年 | 1篇 |
2008年 | 4篇 |
2007年 | 1篇 |
2006年 | 2篇 |
2005年 | 4篇 |
2004年 | 3篇 |
2003年 | 4篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1995年 | 1篇 |
排序方式: 共有46条查询结果,搜索用时 15 毫秒
1.
Cloning of the cDNA encoding the urotensin II precursor in frog and human reveals intense expression of the urotensin II gene in motoneurons of the spinal cord 总被引:14,自引:0,他引:14
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yolaine Coulouarn Isabelle Lihrmann Sylvie Jegou Youssef Anouar Herve Tostivint Jean Claude Beauvillain J. Michael Conlon Howard A. Bern Hubert Vaudry 《Proceedings of the National Academy of Sciences of the United States of America》1998,95(26):15803-15808
Urotensin II (UII) is a cyclic peptide initially isolated from the caudal neurosecretory system of teleost fish. Subsequently, UII has been characterized from a frog brain extract, indicating that a gene encoding a UII precursor is also present in the genome of a tetrapod. Here, we report the characterization of the cDNAs encoding frog and human UII precursors and the localization of the corresponding mRNAs. In both frog and human, the UII sequence is located at the C-terminal position of the precursor. Human UII is composed of only 11 amino acid residues, while fish and frog UII possess 12 and 13 amino acid residues, respectively. The cyclic region of UII, which is responsible for the biological activity of the peptide, has been fully conserved from fish to human. Northern blot and dot blot analysis revealed that UII precursor mRNAs are found predominantly in the frog and human spinal cord. In situ hybridization studies showed that the UII precursor gene is actively expressed in motoneurons. The present study demonstrates that UII, which has long been regarded as a peptide exclusively produced by the urophysis of teleost fish, is actually present in the brain of amphibians and mammals. The fact that evolutionary pressure has acted to conserve fully the biologically active sequence of UII suggests that the peptide may exert important physiological functions in humans. 相似文献
2.
Laurent Sulpice Michel Rayar Bruno Turlin Eveline Boucher Pascale Bellaud Mireille Desille Bernard Meunier Bruno Clément Karim Boudjema Cédric Coulouarn 《The Journal of surgical research》2014
Background
Recently, we identified a gene signature of intrahepatic cholangiocarcinoma (ICC) stroma and demonstrated its clinical relevance for prognosis. The most upregulated genes included epithelial cell adhesion molecule (EpCAM), a biomarker of cancer stem cells (CSC). We hypothesized that CSC biomarkers could predict recurrence of resected ICC.Methods
Both functional analysis of the stroma signature previously obtained and immunohistochemistry of 40 resected ICC were performed. The relationships between the expression of CSC markers and clinicopathologic factors including survival were assessed by univariate and multivariable analyzes.Results
Gene expression profile of the stroma of ICC highlighted embryonic stem cells signature. Immunohistochemistry on tissue microarray showed at a protein level the increased expression of CSC biomarkers in the stroma of ICC compared with nontumor fibrous liver tissue. The overexpression of EpCAM in the stroma of ICC is an independent risk factor for overall (hazard ratio = 2.6; 95% confidence interval, 1.3–5.1; P = 0.005) and disease-free survival (hazard ratio = 2.2; 95% confidence interval, 1.2–4.2; P = 0.012). In addition, the overexpression of EpCAM in nontumor fibrous liver tissue is closely correlated with a worst disease-free survival (P = 0.035).Conclusions
Our findings provide new arguments for a potential role of CSC on ICC progression supporting the idea that targeting CSC biomarkers might represent a promise personalized treatment. 相似文献3.
4.
5.
Marie Soressi Shannon P. McPherron Michel Lenoir Tamara Dogand?i? Paul Goldberg Zenobia Jacobs Yolaine Maigrot Naomi L. Martisius Christopher E. Miller William Rendu Michael Richards Matthew M. Skinner Teresa E. Steele Sahra Talamo Jean-Pierre Texier 《Proceedings of the National Academy of Sciences of the United States of America》2013,110(35):14186-14190
Modern humans replaced Neandertals ∼40,000 y ago. Close to the time of replacement, Neandertals show behaviors similar to those of the modern humans arriving into Europe, including the use of specialized bone tools, body ornaments, and small blades. It is highly debated whether these modern behaviors developed before or as a result of contact with modern humans. Here we report the identification of a type of specialized bone tool, lissoir, previously only associated with modern humans. The microwear preserved on one of these lissoir is consistent with the use of lissoir in modern times to obtain supple, lustrous, and more impermeable hides. These tools are from a Neandertal context proceeding the replacement period and are the oldest specialized bone tools in Europe. As such, they are either a demonstration of independent invention by Neandertals or an indication that modern humans started influencing European Neandertals much earlier than previously believed. Because these finds clearly predate the oldest known age for the use of similar objects in Europe by anatomically modern humans, they could also be evidence for cultural diffusion from Neandertals to modern humans. 相似文献
6.
Ricard JD Eveillard M Martin Y Barnaud G Branger C Dreyfuss D 《American journal of infection control》2011,39(7):605-607
The impact of tracheal suctioning with an open or a closed system on equipment and health care workers contamination with multidrug-resistant pathogens was compared. Only the closed system reduced hand and equipment contamination during tracheal suctioning. This equipment could be systematically used to reduce risk of cross contamination in the intensive care unit. 相似文献
7.
The release of pro‐inflammatory cytokines is mediated via mitogen‐activated protein kinases rather than by the inflammasome signalling pathway in keratinocytes
下载免费PDF全文
![点击此处可从《Clinical and experimental pharmacology & physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
8.
9.
10.
Autophagy is an evolutionarily conserved cellular degradation pathway involving the digestion of intracellular components via the lysosomal pathway. The autophagic pathway constitutively maintains cellular homeostasis by recycling cytoplasmic organelles and proteins, but it is also stimulated by environmental stress conditions, such as starvation, oxidative stress, and the accumulation of misfolded proteins. It also acts as a cellular defense mechanism against microorganisms by contributing to both the innate and adaptive immunity, and by eliminating intracellular pathogens (xenophagy). There is growing evidence that host cells try to control Herpesvirus infections by activating the autophagic machinery. However, it is well-known that Herpesviruses are smart pathogens and several, such as HSV-1, HCMV and HHV-8, are known to have developed numerous defense strategies for evading the host's immune response. Inhibition of the antiviral autophagic mechanism has also been reported. Autophagy has also been shown to enhance the major histocompatibility complex presentation of at least two viral proteins, the EBV-encoded EBNA-1 and the HSV-1 encoded gB. In this review, we present an overview of recent advances in our understanding of the complex interplay between autophagy and Herpesviruses. 相似文献