紫外线照射后哺乳动物细胞DNA的期外合成

本实验用~3H-TdR掺入,液体闪烁测量法测定细胞DNA期外合成的方法,测定了小鼠和大鼠的肠淋巴结细胞、人和大鼠外周血淋巴细胞、S-180V肿瘤细胞等经紫外线照射后的UDS,观察到UDS掺入量随辐照度增加而增加。在同一辐照剂量下,以小鼠肠淋巴结细胞的UDS值最高,其次是人外周血淋巴细胞和S-180V肿瘤细胞,而大鼠外周血淋巴细胞和肠淋巴结细胞最低。     

阶段性健康教育对老年食管癌患者术后配合行为的影响

目的探讨阶段性健康教育对老年食管癌患者术后配合行为的影响。方法将51例患者按入院先后分为观察组（26例）和对照组（25例）。观察组实施阶段性健康教育，即住院当日、术前1～3d、术后回病房、术后第1天、拔除胃管当日、出院前1d，由管床护士实施不同内容的健康教育；对照组实施传统健康教育。术后1周评价两组患者术后的配合行为及术后并发症的发生率。结果观察组术后配合行为显著优于对照组（均P〈0．01）；并发症发生率显著低于对照组（P〈0．05）。结论对老年食管癌手术患者实施阶段性健康教育，可提高患者的术后配合行为，降低并发症的发生。     

Related factors of cognitive impairment after stroke

BACKGROUND: Cognitive impairment after stroke associates with various factors, such as age, educational years, etc. Besides concerning about the recovery of limb function after stroke, we should also focus on the rehabilitation of cognition. Moreover, we'd better pay attention to the control of all the risk factors of stroke, and improve the quality of life in stroke patients. OBJECTIVE: To analyze the factors that affect cognitive impairment after stroke. DESIGN: A related factors analysis. SETTINGS: Department of Neurology of Dalian Port Hospital and Dalian Second People's Hospital. PARTICIPANTS: Totally 148 stroke inpatients were selected from Department of Neurology, Dalian Port Hospital and Dalian Second People's Hospital from April 2004 to December 2005, including 100 males and 48 females, aging 45-75 years with an average age of (67±8) years; Their educational years ranged 2-10 years with an average of (6.1±3.7) years; The disease course ranged 15-30 days; All were right-handed. Inclusive criteria: All were accorded with the diagnostic standard set by the Fourth National Academic Meeting for Cerebrovascular Disease; Confirmed by CT or MRI; Informed consents were obtained from all the subjects. METHODS: After the disease conditions were stable, the patients were assessed with Wechsler memory scale (WMS) and Wisconsin card sorting test (WCST). WMS included forward and backward recitation of numbers and short-term memory (verbal memory, visual recognition). The number of times for correct and wrong classifications in WCST and the time to complete the trail making tests A and B were recorded. The focal volume, area and layer were recorded at 24 hours after admission. The general data of the patients were recorded, including name, sex, age, educational years, history of hypertension, history of diabetes mellitus. Electroencepalograph (EEG) was examined to record the wave shape, blood lipids were detected, and the cognition related indexes were analyzed with the Pearson correlation analysis. The correlation between cognitive indexes after stroke and the influencing factors were analyzed with multiple linear regression analysis. MAIN OUTCOME MEASURES: ① Correlation between cognitive indexes and imaging indexes; ② Influencing factors for cognitive indexes after stroke. RESULTS: All the 148 stroke patients were involved in the analysis of results. ① Correlation between cognitive indexes and CT indexes: There were obvious negative correlations between numerical symbol and focal layer (r =-0.234, P < 0.05), as well as between verbal memory and the focal area and volume (r =-0.363, -0.279, P < 0.05); Trail making test A had obvious positive correlation with focal area and volume (r =0.256, 0.256, P < 0.05). Results of multiple linear regression analysis: Correct classification was correlated with triglyceride (partial wave and θ wave (partial regression coefficient=0.231-0.908, P < 0.05); Verbal memory was correlated with EEG α wave, focal volume, sex, educational years and diabetes mellitus (partial regression coefficient=0.219-1.017, P < 0.05-0.01). Visual recognition had correlation with educational years and hypertension (partial regression coefficient=0.326, -1.163, P < 0.01). Trail making tests A and B were correlated with focal volume (partial regression coefficient=4.680, -18.561, P < 0.05). CONCLUSION: The factors that affect the cognitive function after stroke include sex, age, educational years, hypertension, diabetes mellitus, triglyceride, EEG wave shape, and the focal area, volume and layer     

病毒性肝炎60例临床与病理分析

对６０例病毒性肝炎患者进行肝穿刺活检，结果发现，临床与病理诊断的符合率为４６．６％，其中，急性肝炎的诊断符合率最低（２３．８％），慢性迁延型肝炎（慢迁肝）为４４．８％；ＨＢｓＡｇ阳性首次发病的患者中，急性乙型肝炎仅为１１．２％（２／１８）；临床诊断的慢性活动型肝炎（慢活肝）仅为病理检出的２５．７％（９／３５）；肝炎患者中脾肿大，凝血酶元时间延长、Ａ／Ｇ值＜１．２、抗ＨＢｃＩｇＭ阳性可作为慢活肝与慢迁肝鉴别的重要指标。这些对提高病毒性肝炎临床分型诊断的准确率及指导临床治疗有意义。     

浅谈药剂学的备课

由于长期从事药剂学的教学工作，在备教材、备学生这2个重要方面积累了一些经验：寻找教材中存在的问题，然后适当地处理教材；找准学生真实的学习起点，为学生设计自主学习的方式，以便于更好地实施教学。     

胆结石对肝脏细胞钙稳态影响与调整的实验研究

用低蛋白饮食方法建立豚鼠胆色素结石模型，共设对照、致石、维生素Ｃ修复、丹参修复和对照修复等５组，规定时间内处死动物，用放射免疫、固相酶联免疫、生物化学等方法检测肝细胞内环—磷酸腺甙（ｃＡＭＰ）、环—磷酸鸟嘌呤（ｃＧＭＰ）、钙调素（ＣａＭ）、钙，三磷酸腺甙酶（Ｃａ２＋－ＡＴＰａｓｅ）、磷酸化酶ａ等水平。致石组豚鼠肝脏细胞内ｃＡＭＰ和磷酸化酶ａ升高，而ｃＧＭＰ，ＣａＭ和Ｃａ２＋－ＡＴＰａｓｅ下降，表明肝细胞钙稳态呈失调状态。维生素Ｃ和丹参可调整肝细胞的上述改变，说明维生素Ｃ和丹参具有维持肝细胞钙稳态的作用。     

东莨菪碱对大鼠下丘脑室旁核精氨酸升压素的影响

目的 观察东莨菪碱对大鼠下丘脑室核精氨酸升压素的影响。方法 以不同浓度Ｓｃｏｐ推挽灌流大鼠下丘脑室旁核 结果 扣留出液中精氨酸升压素含量随推挽时间延长而增多。结论Ｓｃｏｐ可促进ＰＶＮ释放内源性ＡＶＰ，这对了解Ｓｃｏｐ抗休克的机理有重大的意义。     

RP—HPLC测定人血浆中洛伐他汀浓度及药动学研究

用ＲＰ－ＨＰＬＣ测定人血浆中洛伐他汀浓度。以甲醇－水（８３∶１７，Ｖ／Ｖ）为流动相，色谱柱采用ＨＹＰＥＲＳＩＬＢＤＳ－Ｃ１８（５μｍ）不锈钢柱，紫外２３７ｎｍ波长检测。血浆样品经环己烷－异丙醇（９５∶５）萃取浓缩后进样测定。洛伐他汀浓度在２．５～８０ｎｇ／ｍｌ范围内线性良好，ｒ＝０．９９６８。测定含洛伐他汀２０．０ｎｇ／ｍｌ的血浆样品，其日内（ｎ＝７）及日间（ｎ＝７）的ＲＳＤ分别为９．８％和８．５％。回收率平均为（１０１．３±５．５）％。测定了１０名健康志愿者单次ｐｏ洛伐他汀片剂８０ｍｇ后不同时间的血药浓度，计算了相应的药动学参数。     

支气管肺隔离症1例报道

男性,28岁,体检发现右下肺肿块1月余,无明显自觉症状.以往有肺结核病史.胸部X线平片(图1)显示右肺下叶后基底段一6 cm×4 cm×5 cm的团块影,边缘清晰,双肺上叶可见陈旧性结核灶.CT增强扫描(图2)显示右下肺肿块,中央CT值约40HU,增强后无强化,肿块内侧可见一粗大的血管断面伴随,血管与肿块关系密切.CT血管造影(CTA)MIP重建显示一较大的血管自膈下发出一直径约1cm的供血动脉,并可见引流的肺静脉影(图3).     

个性化护理干预对妇科恶性肿瘤手术后生活质量的影响

[目的]探讨个性化护理干预对妇科恶性肿瘤手术后生活质量的影响。[方法]将46例在我科因妇科恶性肿瘤手术的患者随机分为实验组和对照组，通过实施个性化护理干预，对两组患者进行SF-36量表和Olson婚姻质量问卷进行测评。[结果]对妇科恶性肿瘤手术后患者进行个性化护理干预后其生活质量明显提高，差异有显著性意义（P〈0．01）。[结论]个性化护理干预能提高妇科恶性肿瘤手术后生活质量。