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排序方式: 共有468条查询结果,搜索用时 15 毫秒
1.
Neutrophil Oxygen Radical Production by Dialysis Membranes 总被引:1,自引:0,他引:1
The ability of different dialysis membranes to activate polymorphonuclearneutrophil oxygen radical production was investigated with chemiluminescence.All the six membranes, namely cuprophan, cellulose acetate,polycarbonate, polysulphone, polyacrilonitrile and polymethylmethacrylatewere able to interact with neutrophils and stimulate their oxygenradical production, the highest responses being seen with polyacrilonitrile,polymethylmethacrylate and polycarbonate. To analyse the roleof complement in this interaction, fresh plasma, heat inactivatedand zymosan-activated plasma were added: with fresh plasma oxygenradical production was stimulated on cuprophan, cellulose acetateand polysulphone, not modified on polycarbonate, and decreasedon polyacrilonitrile and polymethylmethacrylate. With heat-inactivatedplasma, the responses were decreased or abrogated on all themembranes except polycarbonate and polymethylmethacrylate, whereaswith zymosanactivated plasma similar responses to fresh plasmawere observed. In addition, when plasma was used to precoatthe membrane, cuprophan, cellulose acetate and polysulphonedisclosed an enhanced neutrophil oxidative burst, while precoatedpolyacrilonitrile and polymethylmethacrylate were less stimulatorythan uncoated membranes. In contrast the precoating of polycarbonatedid not modify oxygen radical production. These data suggestthat neutrophil activation occurs by direct membrane neutrophilinteraction. Plasmatic factors modulate this interaction butcomplement seems involved on cellulosic and polysulphone membranesonly. Therefore, it appears that oxygen radicals produced fromcontact of neutrophils with the dialysis membrane might playan initial and/or additional role in the events occurring atthe initiation of haemodialysis. 相似文献
2.
3.
Yersin C; Bovet P; Wauters J; Schorderet D; Pescia G; Paccaud F 《Nephrology, dialysis, transplantation》1997,12(10):2069-2074
Background: As little such data is available in
African populations, we investigated the prevalence of ADPKD and the impact
of the disease in the Seychelles islands, where approximately 75% of the
population is of African descent and 30% of Caucasian or mixed descent.
Method: Prevalent cases were identified over a 3-year
period by requesting all the doctors in the country (most of them are
employed within a national health system) to refer all presumed or
confirmed cases and by systematically examining the family members of all
confirmed cases. The diagnosis was based on standard criteria including
ultrasonograhic findings and family history. Results:
Forty-two cases were identified in this population of 74 331 inhabitants, a
total prevalence (per 100 000 total population) of 57 (95% CI, 41-76). All
but one of the cases were of Caucasian descent so that the prevalence rates
of the disease in the populations of Black and Caucasian descents were
respectively 2 (0-11) and 184 (132-249). The prevalence rates of the
gene(s) carriers were estimated to be 75 (45-117) in the total population
respectively 6 (0-33) and 236 (140-372) in the Black and Caucasian
populations. Haplotype analysis in 58 cases from three families showed a
common DNA fragment in all affected individuals. Cases had significantly
higher blood pressure compared to the general population and 21% had serum
creatinine higher than 120 &mgr;mol/l. Among the established pedigrees,
mean age of death between 1960 and 1995 (haemodialysis was introduced in
1992) was younger in subjects with than those without ADPKD (50.5 vs 67.7
years; P<0.001). Conclusions: In the
Seychelles, ADPKD clusters in the Caucasian population (possibly a founder
effect), is rare in individuals of black descent, and is associated with
substantial clinical and survival impact. 相似文献
4.
C Alonso-Vega N Wauters D Vermeylen M F Muller E Serruys 《Journal of clinical microbiology》1997,35(1):286-287
We report the case of a 20-day-old full-term baby, born to a mother who had had an uncomplicated pregnancy and delivery, who died 13 days after the onset of meningitis. Mycoplasma hominis was the sole agent repeatedly recovered from cerebrospinal fluid and from postmortem brain tissue. 相似文献
5.
Distribution of nocardia species in clinical samples and their routine rapid identification in the laboratory 总被引:1,自引:0,他引:1
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Wauters G Avesani V Charlier J Janssens M Vaneechoutte M Delmée M 《Journal of clinical microbiology》2005,43(6):2624-2628
Eighty-six Nocardia strains isolated from clinical samples in Belgium were identified by 16S rRNA gene sequencing. Eighty-three (96%) strains belonged to only six Nocardia species: N. farcinica (38 [44%]), N. nova (19 [22%]), N. cyriacigeorgica (13 [15%]), N. brasiliensis (6 [6.9%]), N. abscessus (5 [5.8%]), and N. paucivorans (2 [2.3%]). A gallery of nine conventional and enzymatic tests was developed for the rapid identification of the most common species isolated during this survey. Pyrrolidonyl aminopeptidase, γ-glutamyl aminopeptidase, α-mannosidase, and α-glucosidase were found to be highly discriminating and could be used to develop an identification scheme. 相似文献
6.
De Baere T Muylaert A Everaert E Wauters G Claeys G Verschraegen G Vaneechoutte M 《Journal of clinical microbiology》2002,40(7):2693-2695
A gram-negative alkaline phosphatase- and pyrrolidone peptidase-positive rod-shaped bacterium (CCUG 45702) was isolated from two aerobic blood cultures from a female cancer patient. No identification could be reached using phenotypic techniques. Amplification of the tRNA intergenic spacers revealed fragments with lengths of 116, 133, and 270 bp, but no such pattern was present in our reference library. Sequencing of the 16S rRNA gene revealed its identity as Moraxella atlantae, a species isolated only rarely and published only once as causing infection. In retrospect, the phenotypic characteristics fit the identification as M. atlantae (formerly known as CDC group M-3). Comparative 16S rRNA sequence analysis indicates that M. atlantae, M. lincolnii, and M. osloensis might constitute three separate genera within the MORAXELLACEAE: After treatment with amoxicillin-clavulanic acid for 2 days, fever subsided and the patient was dismissed. 相似文献
7.
Nucleotide sequence of yst, the Yersinia enterocolitica gene encoding the heat-stable enterotoxin, and prevalence of the gene among pathogenic and nonpathogenic yersiniae. 总被引:16,自引:8,他引:16
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The gene encoding the heat-stable enterotoxin (yst) was cloned from the chromosome of Yersinia enterocolitica W1024 (serotype O:9), and the nucleotide sequence was determined. The yst gene encodes a 71-amino-acid polypeptide. The C-terminal 30 amino acids of the predicted protein exactly correspond to the amino acid sequence of the toxin extracted from culture supernatants (T. Takao, N. Tominaga, and Y. Shimonishi, Biochem. Biophys. Res. Commun. 125:845-851, 1984). The N-terminal 18 amino acids have the properties of a signal sequence. The central 22 residues are removed during or after the secretion process. This organization in three domains (Pre, Pro, and mature Yst) resembles that of the enterotoxin STa of Escherichia coli. The degree of conservation between the E. coli and Y. enterocolitica toxins is much lower in the Pre and the Pro domains than in the mature proteins. The mature toxin of Y. enterocolitica is much larger than that of E. coli, but the active domain appears to be highly conserved. The yst gene of Y. enterocolitica introduced in E. coli K-12 directed the secretion of an active toxin. The cloned yst gene was used as an epidemiological probe among a collection of 174 strains representative of all Yersinia species except Yersinia pestis and numerous Y. enterocolitica subgroups. In Y. enterocolitica, there was a clear-cut difference between pathogenic and nonpathogenic strains: 89 of 89 pathogenic and none of 51 nonpathogenic strains contained yst-homologous DNA, suggesting that Yst is involved in pathogenesis. Among the other Yersinia species, only four strains of Yersinia kristensenii had DNA homologous to yst. 相似文献
8.
Detection of pYV+ Yersinia enterocolitica isolates by P1 slide agglutination. 总被引:4,自引:2,他引:4
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M P Sory J Tollenaere C Laszlo T Biot G R Cornelis G Wauters 《Journal of clinical microbiology》1990,28(11):2403-2408
Rabbit polyclonal antisera were raised against the pYV-encoded outer membrane protein P1 of five Yersinia enterocolitica strains belonging to serogroups O:3, O:5,27, O:8, and O:9. Analysis of these strains with the sera showed that P1 presented at least six different antigenic factors. Two of the serum specimens were chosen to test the P1 agglutinability of 797 strains isolated from various sources. This technique appeared to be more reliable than autoagglutination and Ca2+ dependency to monitor the presence of the pYV plasmid. Hence, we propose this P1-mediated agglutination as a new and easy virulence test. 相似文献
9.
Bacteremia due to a novel Microbacterium species in a patient with leukemia and description of Microbacterium paraoxydans sp. nov
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Laffineur K Avesani V Cornu G Charlier J Janssens M Wauters G Delmée M 《Journal of clinical microbiology》2003,41(5):2242-2246
A yellow-pigmented coryneform rod was isolated from the blood of a child with acute lymphoblastic leukemia who was perfused with a central venous catheter. The culture bottles were positive twice, at a 2-month interval. The isolate was identified as a Microbacterium sp. and studied along with five other similar strains. Phenotypic, chemotaxonomic, and genetic characteristics indicated that they are closely related to Microbacterium oxydans but that they belong to a distinct species, for which the name Microbacterium paraoxydans sp. nov. is proposed. The type strain of M. paraoxydans is CF36(T) = DSM 15019(T). The G+C content of its DNA is 69.9 mol%. 相似文献
10.
Wuyts W Roland D Lüdecke HJ Wauters J Foulon M Van Hul W Van Maldergem L 《American journal of medical genetics》2002,113(4):326-332
Multiple exostoses represent a genetically heterogeneous disorder that may occur isolated or as part of a complex contiguous gene syndrome such as Langer-Giedion syndrome on chromosome 8 and the proximal 11p deletion syndrome on chromosome 11. Here we describe a boy with multiple exostoses, hypertrichosis, mental retardation, and epilepsy due to a de novo deletion on chromosome 8q24. Molecular analysis revealed that the deletion interval overlaps with the Langer-Giedion syndrome and involves the EXT1 gene and additional genes located distal to EXT1, but probably not encompassing the TRPS1 gene located proximal to EXT1. 相似文献