全文获取类型
收费全文 | 567篇 |
免费 | 48篇 |
国内免费 | 32篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 28篇 |
妇产科学 | 9篇 |
基础医学 | 58篇 |
口腔科学 | 13篇 |
临床医学 | 92篇 |
内科学 | 193篇 |
皮肤病学 | 4篇 |
神经病学 | 39篇 |
特种医学 | 64篇 |
外科学 | 21篇 |
综合类 | 23篇 |
预防医学 | 24篇 |
眼科学 | 9篇 |
药学 | 51篇 |
中国医学 | 4篇 |
肿瘤学 | 14篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 7篇 |
2020年 | 3篇 |
2019年 | 9篇 |
2018年 | 10篇 |
2017年 | 9篇 |
2016年 | 8篇 |
2015年 | 7篇 |
2014年 | 19篇 |
2013年 | 21篇 |
2012年 | 12篇 |
2011年 | 23篇 |
2010年 | 26篇 |
2009年 | 23篇 |
2008年 | 29篇 |
2007年 | 42篇 |
2006年 | 29篇 |
2005年 | 19篇 |
2004年 | 14篇 |
2003年 | 7篇 |
2002年 | 6篇 |
2001年 | 9篇 |
2000年 | 4篇 |
1999年 | 17篇 |
1998年 | 14篇 |
1997年 | 18篇 |
1996年 | 18篇 |
1995年 | 14篇 |
1994年 | 21篇 |
1993年 | 15篇 |
1992年 | 15篇 |
1991年 | 16篇 |
1990年 | 14篇 |
1989年 | 15篇 |
1988年 | 13篇 |
1987年 | 18篇 |
1986年 | 11篇 |
1985年 | 13篇 |
1984年 | 11篇 |
1983年 | 6篇 |
1982年 | 3篇 |
1981年 | 12篇 |
1980年 | 3篇 |
1979年 | 10篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1975年 | 8篇 |
1974年 | 2篇 |
排序方式: 共有647条查询结果,搜索用时 15 毫秒
1.
Neuroimaging in Pineal Tumors 总被引:4,自引:0,他引:4
F Reis MD AV Faria MD PhD VA Zanardi MD PhD JR Menezes MD F Cendes MD PhD LS Queiroz MD PhD 《Journal of neuroimaging》2006,16(1):52-58
BACKGROUND AND PURPOSE: The authors report radiological findings in 11 tumors in the pineal region, which were histologically diagnosed as germinomas, pineocytomas pineoblastomas, ependymomas, teratomas, and astrocytomas. METHODS: Computed tomography (CT) was performed in seven patients and magnetic resonance imaging (MRI) was performed in all patients. RESULTS: CT showed a solid or solid/cystic mass with variable contrast enhancement. MRI showed a heterogeneous mass, with hypointense signal on T1 and iso/hyperintense signal on T2-weighted images (WI) and gadolinium enhancement. Extension to adjacent structures occurred in five patients and spread through the cerebral spinal fluid (CSF) in two. CONCLUSIONS: Pineal region tumors have no pathognomonic imaging pattern. MRI and CT are complementary in diagnosis and are important to determine localization, extension, and meningeal spread. 相似文献
2.
3.
4.
5.
Jiang WZ Jin NY Li ZJ Zhang LS Wang HW Zhang YJ Han WY 《第二军医大学学报》2006,27(4):434-434
To express the core protein of HIV-1 of Chinese prevalent strain (HIV-1 (CN)) in Pichia pastoris, the fulllength gag gene was inserted into the secretory expression vector pHILS1. Linearized recombinant plasmid pHILGAG by Sail was electrotransformed into the yeast strain GS115, and the yeast transformants were identified by PCR. To induce the interest protein to be expressed, the PCR positive transformants were inoculated in the medium of BMGY and BMMY, mRNA of the strain was detected by RT-PCR, and the expressed protein was analyzed by SDS-PAGE, Western blotting and thin layer scanning. mRNA (1.3 kb) was amplified by RT-PCR. SDS-PAGE and Western blotting analysis showed that the molecular mass of the expressed protein was 55 kD, which was similar to the expected value, and the expressed protein could react with McAb to HIV-1 p24. Thin layer scanning analysis demonstrated that the whole amount of the expressed protein was approximately 13 % of the soluble protein in the supernatant. The recombinant yeast had good genetic stability. The optimal expression conditions of the engineering yeast were as follows: BMMY medium, 80-90% of dissolved oxygen, 1% methanol, and 3-day-cultivation course. Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography. The purity of the interest protein was up to 85 %. After the purified proteins were inoculated into BALB/c mice, the anti-HIV-1 antibodies in the immunized mice could be detected by Western blotting. 相似文献
6.
Alice E. Collette Kristen Wann Meredith L. Nevin Karen Rique Grant Tarrant Lorraine A. Hickey 《Journal of interprofessional care》2017,31(4):470-478
Interprofessional collaboration is a key element in providing safe, holistic patient care in the acute care setting. Trended data at a community hospital indicated opportunities for improvement in collaboration on micro, meso, and macro levels. The aim of this survey study was to assess the current state of collaboration between frontline nurses and physicians at a non-academic acute care hospital. A convenience sample of participants was recruited with a final respondent sample of 355 nurses and 82 physicians. The results indicated that physicians generally perceived greater collaboration than nurses. Physician ratings did not vary by primary practice area, whereas nurse ratings varied by clinical practice area. Nurse ratings were the lowest in the operating room and the highest in the emergency department. Text-based responses to an open-ended question were analysed by role and coded by two independent research teams. Emergent themes emphasised the importance of rounding, roles, respect, and communication. Despite recognition of the need for improved collaboration and relational behaviours, strategies to improve collaborative practice must be fostered at the meso level by organisational leaders and customised to address micro-level values. At the study site, findings have been used to address and improve collaboration towards the goal of becoming a high reliability organisation. 相似文献
7.
8.
9.
10.