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排序方式: 共有254条查询结果,搜索用时 15 毫秒
1.
Intracytoplasmic lumina (ICL) in human oviduct epithelium were investigated with transmission electron microscopy. ICL were found in 43 out of 60 cases examined. They were ultra-structurally characterized by microvilli lining the lumina, periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining-positive finely granular material in the lumina, and secretory vesicles in the cytoplasm surrounding the lumina. Although ICL were observed at various heights within the epithelium, they were mainly seen in basally located cells that did not face the oviduct lumen. Various stages of formation and development of ICL were observed in the basally located epithelial cells with secretory activities. Primary ICL were originated in the cytoplasm where the secretory granules were aggregated with smooth-surfaced tubular vesicles. Electron microscopic observations after PA-TCH-SP staining revealed that ICL were formed by fusion of the secretory granules with the tubular vesicles. ICL were enlarged into round profiles by further fusion of secretory granules and tubular vesicles, and subsequently opened to the oviduct lumen, or fused to each other to develop into large extracellular cysts within the epithelium. 相似文献
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Matsuda T Ihara M Inoguchi H Kwon IK Takamizawa K Kidoaki S 《Journal of biomedical materials research. Part A》2005,73(1):125-131
To fabricate a "mechano-active" tubular scaffold of nonwoven mesh-type small-diameter artificial graft made of the synthetic durable elastomer, segmented polyurethane, the fabrication technique of electrospinning on a mandrel under a high rotation speed and transverse movement was used. Emphasis was placed on how the rotation speed of the mandrel and the fusion or welding states of fibers at contact points affect the compliance (ease of intraluminal pressure-dependent circumferential inflation) and Young's modulus determined by uniaxial stretching in the longitudinal and circumferential directions. The results showed that a high rotation speed is attributed to exhibit isotropic mechanical properties in the entire range of applied strain but reduces the compliance, and a high fusion state, which is produced using a mixed solvent with a high content of high-boiling-point solvent, reduces the compliance but is expected to exhibit high durability in a continuously loaded pulsatile stress field in an arterial circulatory system. 相似文献
4.
Radioimmunoassay and immunohistochemical studies were performed on the occurrence and distribution of parvalbumin-like immunoreactivity (PA-LI) in developing rat cerebrum, cerebellum and retina. No PA-LI was detected in the nervous tissues of the newborn animals. In the cerebrum, the PA-LI appeared in non-pyramidal neurons at the 2nd postnatal week and increased linearly until the 8th week. In the cerebellum, a rapid increase in the PA-LI took place at the 2nd week, with an enrichment of the antigen to Purkinje neurons. In the retina, amacrine cells contained PA-LI, the levels of which increased from the 2nd to 4th week. Regulation of intracellular Ca2+ concentration may be one of the important factors for the maturation of the central nervous system. 相似文献
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A 42-year-old woman complicated with neurofibromatosis underwent both balloon percutaneous transluminal angioplasty (PTA) of the iliac artery and femoropopliteal (FP) bypass grafting for critical lower limb ischemia. Seven months after the initial intervention, a recurrence of stenosis in the iliac artery and at the anastomoses of the FP bypass necessitated both PTA and a repeat thrombectomy and finally resulted in the amputation of her left thigh. This is a rarely documented case of chronic arterial occlusion associated with neurofibromatosis, in which the prognosis of arterial reconstruction for such patients is suggested to be poor. 相似文献
7.
It has been shown that cigarette smoking increases blood pressure (BP) and heart rate (HR), and decreases muscle sympathetic nerve activity (MSNA) in healthy young smokers. The decrease in MSNA might be secondary to baroreflex responses to the pressor effect. We tested the hypothesis that cigarette smoking increases MSNA in smokers with impaired baroreflex function. The effects of cigarette smoking on BP, HR, forearm blood flow (FBF), forearm vascular resistance (FVR), and MSNA were examined in 14 patients with stable effort angina (59+/-3 years, group CAD) and 10 healthy smokers (23+/-1 years, group C). In group CAD, the arterial baroreflex sensitivity (BRS) was significantly lower than in group C (4.7+/-0.8 versus 15.1+/-2.2 msec/mmHg, P<0.01). In both groups, cigarette smoking increased the plasma concentration of nicotine, systolic and diastolic BP, HR, and FVR significantly (P<0.01), but decreased FBF significantly (P<0.01). After smoking, MSNA was decreased significantly in group C (from 35.2+/-3.5 to 23.5+/-3.2 bursts/100 beats, P<0.01), but increased significantly in group CAD (from 48.8+/-5.4 to 57.3+/-5.5 bursts/100 beats, P<0.01). There was significant correlation between BRS and changes in MSNA (r= -0.62, P<0.01). Cigarette smoking increased MSNA in smokers with impaired baroreflex function. This demonstrates that cigarette smoking stimulates sympathetic nerve activity by both a direct peripheral effect and a centrally mediated effect. 相似文献
8.
K+-activated para-nitrophenylphosphatase (K+-pNPPase) was characterized kinetically in isolated adult dog heart myocytes. The results show that: (i) the Km and Vmax for K+ activation were increased by increasing concentrations of Mg2+ and para-nitrophenylphosphate (pNPP); the highest Vmax/Km value was obtained at 5 m
Mg2+, 5 m
pNPP and 10 m
K+; (ii) the optimal molar ratio of Mg2+ to pNPP was 1 when the concentration of K+ was 10 m
and that of pNPP was higher than 1.25 m
; (iii) when the molar ratio of Mg2+ to pNPP was kept at 1 and the K+ concentration was 10 m
, increases in Mg2+ and pNPP concentrations increased the enzyme activity sigmoidally with a Hill coefficient of 1.7 and a S0.5 value of 2.3 m
; (iv) Na+ at low concentrations (< 10 m
) activated, while Na+ at high concentrations (> 10 m
) inhibited K+-pNPPase activity when K+ concentrations were greater than 1 m
; (v) Ca2+ at low concentrations (0.1 to 0.2 m
) inhibited K+-pNPPase activity by competing with K+, while Ca2+ at high concentrations (0.5 to 1.0 m
) inhibited K+-pNPPase activity by mixed (both competitive and non-competitive) competition with K+; (vi) ouabain inhibited K+-pNPPase activity with a Hill coefficient of 0.59 and a S0.5 value of 4.8 × 10−7
; the inhibitory effect of ouabain (< 10−6
) was decreased in the presence of Ca2+ (0.1–0.5 m
). These results demonstrate that isolated adult heart myocytes possess K+-pNPPase activity which can be used as a sensitive and specific probe for studies of the myocardial sodium pump ATPase enzyme system. 相似文献
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10.
Battsetseg Batchuluun Toyoshi Inoguchi Noriyuki Sonoda Shuji Sasaki Tomoaki Inoue Yoshinori Fujimura Daisuke Miura Ryoichi Takayanagi 《Atherosclerosis》2014