Mise au point sur l'analyse par chromatographie par exclusion stérique en milieu aqueux de télomères de l'acide acrylique

In the case of water soluble polymers, the use of size exclusion chromatography (SEC) for the determination of the molecular weight involves numerous difficulties. In order to analyse and determine the molecular weight of acrylic acid telomers we have first tried to obtain a satisfactory and reproducible separation. In this particular case, low-molecular-weight standards are not commercially available. Therefore, we decided to prepare standards based on acrylic acid, either by telomerization with a fluorinated telogen or by polymerization with an initiator bearing a fluorinated group. A calibration curve was obtained from the standards. Telomers of acrylic acid with thioglycolic acid were analysed. This is a general method for determination of DP _n by SEC when there is no standard for the polymers. It can be used in a wide range of DP _n from 1 to 700.     

Multidisciplinary approach to depression. Preliminary results

In the present study, clinical interview, Visual Evoked Potentials, psychological tests, TRH test, plasmatic MHPG dosage were investigated in 30 depressed patients with major depressive disorders. All the explorations were realised before and after 28 days of an antidepressant treatment. Thanks to analysis we found out the value of psychological tests. The study of evoked potentials brings to light the existence of electrophysiological variables, subordinate to the clinical state. On the contrary, "TRH Test" does not give us any useful indication, and plasmatic MOPEG dosage, although it confirms the existence of two groups of depressed patients, does not suggest any relationship with evolutive types.     

Kyste hydatique du foie ouvert dans la vésicule biliaire : à propos d’un cas

The opening in the intra hepatic bile ducts is the most common complication of hepatic hydatid cyst. The opening in the gallbladder is unusual.We report the case of a 65-year-old woman with complaints of right upper quadrant pain and fever. Various explorations have found a hydatid acute cholecystitis due to an opening of a hydatid cyst of the liver in the gallbladder. Treatment was surgical.     

Immunohistochemical analysis of c-erbB-2, Bcl-2, p53, p21WAF1/Cip1, p63 and Ki-67 expression in hydatidiform moles

Hydatidiform moles (HM) are characterized by an abnormal proliferating trophoblast with a potential for a malignant transformation. Similar to other human tumors, trophoblastic pathogenesis is likely a multistep process involving several molecular and genetic alterations. The study was performed to investigate the expression patterns of c-erbB-2 and Bcl-2 oncoproteins, p53, p21^WAF1/CIP1 and p63 tumor suppressor proteins and Ki-67 cell proliferation marker in HM.We conducted a retrospective study of 220 gestational products, including 39 hydropic abortions (HA), 41 partial HM (PHM) and 140 complete HM (CHM). The expression of c-erbB-2, Bcl-2, p53, p21^WAF1/CIP1, p63 and Ki-67 was investigated by immunohistochemistry on archival tissues. c-erbB-2 expression was observed in three PHM and 10 CHM. Bcl-2 immunostaining was significantly higher in PHM (61%) and CHM (70.7%) compared with HA (7.7%, p?=? 0.001 and p?<? 0.0001, respectively). p53 expression was stronger in CHM (73.6%) compared with PHM (24.4%, p < 0.0001) and HA (12.8%, p < 0.0001). p21^WAF1/CIP1 staining was observed as well in molar and non-molar gestations (p?>? 0.05). p63 immunoexpression was significantly described in CHM (85.7%) and PHM (78%) compared with HA (10.2%, p < 0.0001 and p = 0.0001, respectively). Ki-67 was significantly expressed in CHM (72.1%) compared with HA (46.2%, p = 0.005).Altered expression of Bcl-2, p53, p63 and Ki-67 reflects the HM pathological development. Immunohistochemical analysis is beneficial to recognize the HM molecular and pathogenic mechanisms. Furthermore, it could serve as a useful adjunct to conventional methods for refining HM diagnosis.