全文获取类型
收费全文 | 760篇 |
免费 | 48篇 |
国内免费 | 17篇 |
专业分类
儿科学 | 31篇 |
妇产科学 | 5篇 |
基础医学 | 121篇 |
口腔科学 | 18篇 |
临床医学 | 81篇 |
内科学 | 163篇 |
皮肤病学 | 10篇 |
神经病学 | 46篇 |
特种医学 | 110篇 |
外科学 | 53篇 |
综合类 | 20篇 |
预防医学 | 90篇 |
眼科学 | 7篇 |
药学 | 34篇 |
中国医学 | 1篇 |
肿瘤学 | 35篇 |
出版年
2021年 | 10篇 |
2020年 | 8篇 |
2019年 | 6篇 |
2018年 | 11篇 |
2016年 | 9篇 |
2015年 | 7篇 |
2014年 | 25篇 |
2013年 | 23篇 |
2012年 | 32篇 |
2011年 | 32篇 |
2010年 | 26篇 |
2009年 | 21篇 |
2008年 | 22篇 |
2007年 | 31篇 |
2006年 | 33篇 |
2005年 | 21篇 |
2004年 | 21篇 |
2003年 | 16篇 |
2002年 | 18篇 |
2001年 | 26篇 |
2000年 | 16篇 |
1999年 | 21篇 |
1998年 | 27篇 |
1997年 | 20篇 |
1996年 | 32篇 |
1995年 | 24篇 |
1994年 | 11篇 |
1993年 | 8篇 |
1992年 | 15篇 |
1991年 | 21篇 |
1990年 | 13篇 |
1989年 | 22篇 |
1988年 | 29篇 |
1987年 | 9篇 |
1986年 | 15篇 |
1985年 | 20篇 |
1984年 | 13篇 |
1983年 | 11篇 |
1982年 | 8篇 |
1981年 | 8篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1978年 | 6篇 |
1977年 | 13篇 |
1976年 | 10篇 |
1975年 | 4篇 |
1974年 | 5篇 |
1972年 | 5篇 |
1968年 | 3篇 |
1935年 | 3篇 |
排序方式: 共有825条查询结果,搜索用时 468 毫秒
1.
Rebecca Carman Lesley Andrew Amanda Devine Jacques Oosthuizen 《Australian and New Zealand journal of public health》2019,43(6):563-569
Objective: To identify behavioural barriers of service provision within general practice that may be impacting the vaccination coverage rates of Aboriginal children in Perth, Western Australia (WA). Methods: A purposive developed survey was distributed to 316 general practices across Perth and three key informant interviews were conducted using a mixed‐methods approach. Results: Of the surveyed participants (n=101), 67.4% were unaware of the low vaccination coverage in Aboriginal children; 64.8% had not received cultural sensitivity training in their workplace and 46.8% reported having inadequate time to follow up overdue child vaccinations. Opportunistic vaccination was not routinely performed by 30.8% of participants. Key themes identified in the interviews were awareness, inclusion and cultural safety. Conclusion: Inadequate awareness of the current rates, in association with a lack of cultural safety training, follow‐up and opportunistic practice, may be preventing greater vaccination uptake in Aboriginal children in Perth. Cultural safety is a critical component of the acceptability and accessibility of services; lack of awareness may restrict the development of strategies designed to equitably address low coverage. Implications: The findings of this study provide an opportunity to raise awareness among clinicians in general practice and inform future strategies to equitably deliver targeted vaccination services to Aboriginal children. 相似文献
2.
A new range of stand magnifiers has been released by the COIL company in the United Kingdom. Examination of these magnifiers reveals that they fail to deliver the rated magnifications labelled prominently on the appliances, as a result of the manufacturer's conformance with the requirements of the German DIN standard and the use of back vertex power (F'v) rather than equivalent dioptric power (Fm) of the magnifier. In this study we provide information on the optometric parameters of these new stand magnifiers that will assist the more accurate specification of improvements in vision expected from their use. 相似文献
3.
4.
Pheochromocytoma and paraganglioma: comparison of MR imaging with CT and I-131 MIBG scintigraphy 总被引:6,自引:0,他引:6
To ascertain the magnetic resonance (MR) imaging characteristics of pheochromocytomas and paragangliomas and to compare MR with computed tomography (CT) and iodine-131 metaiodobenzylguanidine (I-131 MIBG), 19 patients (18 with pheochromocytomas, one with a paraganglioma) were studied. The 18 patients with pheochromocytomas had had positive findings with I-131 MIBG scintigraphy. Abdominal pheochromocytomas were generally hypointense compared with normal liver on T1-weighted MR images and extremely hyperintense on T2-weighted MR images. MR imaging was preferable to CT in the evaluation of primary pheochromocytomas due to superior tissue characterization, particularly in the patient with hypertension and borderline catecholamine levels. For patients with recurrent or metastatic disease, the data suggest that I-131 MIBG scintigraphy is the examination of choice. 相似文献
5.
6.
7.
8.
Chaperonin-mediated assembly of wild-type and mutant subunits of human propionyl-CoA carboxylase expressed in Escherichia coli 总被引:1,自引:0,他引:1
We developed a bacterial expression system for the human alpha and beta
cDNAs of propionyl-CoA carboxylase (PCC). These cDNAs (less the putative
mitochondrial matrix targeting presequences) were co-expressed in
Escherichia coli on one plasmid vector with each cDNA having its own
IPTG-inducible promoter. Only negligible amounts of active PCC were
measured despite the presence of both alpha and beta subunits as indicated
by Western blot analysis and the almost complete biotinylation of the alpha
subunit. Co-expression of this plasmid with a second plasmid vector
over-expressing the E. coli chaperonin proteins, groES and groEL, resulted
in a several hundred-fold increase in PCC specific activity, to a level
comparable with that found in crude human liver extracts. PCC was partially
purified on monomeric avidin affinity resin and the presence of both alpha
and beta subunits was demonstrated, thereby confirming the assembly of both
subunits into an active enzyme. Deficiency of either alpha PCC or beta PCC
results in propionic acidemia, an autosomal recessive disorder. We used
this expression system to characterize one missense mutation previously
described in five Japanese alleles, namely C1283T (Thr428lle) in beta PCC.
This mutation, when expressed in E.coli under the same conditions as that
of wild-type PCC, had null activity, despite the presence of assembled
alpha PCC and beta PCC subunits. This bacterial expression system can be
useful for analysis of either alpha PCC or beta PCC mutations. Our findings
indicated that the groES and groEL chaperonin proteins were essential for
folding and assembly of the human PCC heteromeric subunits.
相似文献
9.
Jolivet-Reynaud C Lésenéchal M O'Donnell B Becquart L Foussadier A Forge F Battail-Poirot N Lacoux X Carman W Jolivet M 《Journal of medical virology》2001,65(2):241-249
Small hepatitis B surface antigen (HBsAg) is considered to be the best marker for the diagnosis of Hepatitis B virus infection. However, HBsAg variants with mutations within the "a" determinant may be poorly or not detected by diagnostic assays. Three anti-HBsAg monoclonal antibodies (6H6B6, 27E7F10, and 2G2G10), directed against conformational epitopes, were tested for their ability to detect the wild-type HBsAg as well as variant forms and their respective epitopes were localised on the HBsAg sequence by using the phage-displayed peptide library technology. Whereas 6H6B6 did not detect mutations T123N, S143L, D144A and G145R, 27E7F10 binding was affected by mutations P120T and G145R. In contrast, 2G2G10 reacted strongly with all tested variants including variant with the G145R mutation. Part of the 6H6B6 epitope was located in the major hydrophilic region (MHR) at residues 101-105, the 27E7F10 epitope (residues 214-219) was located near the C-terminal end of the antigen and the 2G2G10 epitope at residues 199-208, within the theoretical fourth transmembrane helix. The 2G2G10 epitope localisation brings information about the HBsAg structure and the validity of established topological models. Finally, 2G2G10 is a valuable tool for HBsAg variant detection that is used as capture phase in a new bioMérieux diagnostic assay, which is currently in development. 相似文献
10.