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排序方式: 共有56条查询结果,搜索用时 15 毫秒
1.
Szcześniak Dorota Rymaszewska Joanna Zimny Anna Sąsiadek Marek Połtyn-Zaradna Katarzyna Smith Eric E. Zatońska Katarzyna Zatoński Tomasz Rangarajan Sumathy Yusuf Salim Szuba Andrzej 《Age (Dordrecht, Netherlands)》2021,43(1):279-295
GeroScience - A complex picture of factors influencing cognition is necessary to be drawn for a better understanding of the role of potentially modifiable factors in dementia. The aim was to assess... 相似文献
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3.
Puvanendiran S Stone S Yu W Johnson CR Abrahante J Jimenez LG Griggs T Haley C Wagner B Murtaugh MP 《Virus research》2011,157(1):92-98
Porcine circovirus (PCV) appeared in 1974 as an unidentified, innocuous viral inhabitant of cell cultures and pigs. Today PCV1 is a contaminant of some human vaccines, and PCV2 is a major pathogen of swine. PCV1 is reportedly ubiquitous in swine but nonpathogenic. Since the interplay of PCV1 and PCV2 in swine might explain variable disease results and shed light on the potential for human exposure, we analyzed in depth the prevalence of PCV1 and PCV2 infection and exposure in the U.S. finishing swine herd. Over 82% of sera from 185 farms were positive for PCV2 by PCR, whereas only 2.4% were positive for PCV1. More than 80% of PCV2 DNA-positive swine were also positive for anti-PCV2 antibodies. PCV1 was only rarely present. Exposure of swine, and therefore humans via pigs, to PCV1 is negligible. We conclude that PCV2 causes a persistent infection in pigs and that PCV1 is absent or rare in swine. 相似文献
4.
Eric E. Smith MD MPH Martin O'Donnell MD PhD Gilles Dagenais MD MSc Scott A. Lear PhD Andreas Wielgosz MD PhD Mukul Sharma MD MSc Paul Poirier MD PhD Grant Stotts MD Sandra E. Black MD Stephen Strother PhD Michael D. Noseworthy PhD PEng Oscar Benavente MD Jayesh Modi MD Mayank Goyal MD Saima Batool MD Karla Sanchez BSc Vanessa Hill Cheryl R. McCreary PhD Richard Frayne PhD Shofiqul Islam MSc Jane DeJesus BSc Sumathy Rangarajan MSc Koon Teo MD PhD Salim Yusuf MBBS DPhil 《Annals of neurology》2015,77(2):251-261
5.
Development of an antigen-capture ELISA for detection of H7 subtype avian influenza from experimentally infected chickens 总被引:4,自引:1,他引:3
Velumani S Du Q Fenner BJ Prabakaran M Wee LC Nuo LY Kwang J 《Journal of virological methods》2008,147(2):219-225
Emergence of highly pathogenic avian influenza H7N1 was due to mutation of low pathogenic avian influenza H7N1 strain, which caused outbreaks in Italy between 1999 and 2000, and resulted in complete mortality of infected poultry. This outbreak places increased importance on the early detection of H7N1 AIV. Here we describe the development of a detection method for H7N1 virus from infected chickens using a specific antigen-capture-ELISA (AC-ELISA). A panel of mAbs was developed against the surface antigen HA of H7N1 AIV strain A/chicken/Singapore/94. The mAbs were screened by immunofluorescence assays, ELISA and immunoblotting. Selected mAbs 5E5 and 8F10 were of isotypes IgM and IgG and were conformation- or linear epitope-specific, respectively. These mAbs were used as capture antibodies for AC-ELISA development. The detection limit was as little as 10(2)-10(3) TCID(50) units of virus derived from tissue culture supernatants. Virus from the tracheal swab samples of experimentally infected chickens was detected from days 3 to 7 post-infection using the AC-ELISA, with results being confirmed by RT-PCR. AIV subtypes H4N1, H5N3 H9N2 and H10N5 did not react in the AC-ELISA but were RT-PCR positive, indicating that this AC-ELISA is specific for H7N1 strains. 相似文献
6.
Mohan S Reddick RL Musi N Horn DA Yan B Prihoda TJ Natarajan M Abboud-Werner SL 《Laboratory investigation; a journal of technical methods and pathology》2008,88(5):515-528
Functional consequences of impaired endothelial nitric oxide synthase (eNOS) activity causing organ-specific abnormalities on a diabetic setting are not completely understood. In this study, we extensively characterized a diabetic mouse model (lepr(db/db)) in which eNOS expression is genetically disrupted (eNOS-/-). The eNOS-/-/ lepr(db/db) double-knockout (DKO) mice developed obesity, hyperglycemia, hyperinsulinemia and hypertension. Analysis of tissues from DKO mice showed large islets in the pancreas and fat droplets in hepatocytes. Interestingly, the aorta was normal and atherogenic lesions were not observed. Abnormalities in the aorta including poor re-endothelialization and increased medial wall thickness were evident only in response to deliberate injury. In contrast, significant glomerular capillary damage in the kidney was identified, with DKO mice demonstrating a robust diabetic nephropathy similar to human disease. The vascular and renal impairments in DKO mice were pronounced despite lower fasting plasma glucose levels compared to lepr(db/db) mice, indicating that eNOS is a critical determinant of hyperglycemia-induced organ-specific complications and their severity in diabetes. Results provide the first evidence that absence of eNOS in diabetes has a greater deleterious effect on the renal microvasculature than on the larger aortic vessel. The DKO model may suggest novel therapeutic strategies to prevent both vascular and renal complications of diabetes. 相似文献
7.
Suresh S Sumathy G Banu MR Kamakshi K Prakash S 《Surgical and radiologic anatomy : SRA》2012,34(7):609-617
Purpose
The purpose of this study is to demonstrate the role of aging\edentulousness on the maxillary arch, the size of the alveolar process, the shape and thickness of the hard palate in the South Indian dry skulls to customize more appropriate treatment of elderly edentulous patients.Methods
One hundred dry skulls were divided into dentate and edentulous groups and were subgrouped into male and female. They were subjected to various morphological and morphometrical analyses.Results
The data have revealed a more significant reduction in the depth and width (p?0.001) of the arch corresponding to the reduction in the size and thickness of the alveolar process (p?0.001) in the edentulous skull. Further, a significant reduction in the size and thickness (p?0.001) of the hard palate was observed in the edentulous skull. The data clearly indicate the effect of tooth loss in the horizontal regression of the maxilla. Furthermore, it may enhance the bone resorption that can ultimately result in the marked reduction of the width and depth of the alveolar arch concomitant with the alteration of the hard palate. These changes may subsequently alter the facial appearance in the elderly population.Conclusion
The observed data from specific group of population may provide relevant data for their comparative analysis between different populations for a better understanding of their regional differences with respect to environmental and social influence. Moreover, the data can provide a better idea to evaluate a promising treatment strategy in prosthodontics and orthodontics in South India. 相似文献8.
9.
Mohan Natarajan Samy L. Habib Robert L. Reddick Caroline R. Delma Krishnan Manickam Thomas J. Prihoda Sherry L. Werner Sumathy Mohan 《Journal of diabetes and its complications》2019,33(1):23-32
Previous studies demonstrated that global deficiency of eNOS in diabetic mice exacerbated renal lesions and that overexpression of eNOS may protect against tissue injury. Our study revealed for the first time overexpression of eNOS leads to disease progression rather than protection. Transgenic mice selectively expressing eNOS in endothelial cells (eNOSTg) were cross bred with Ins2Akita type-1 (AK) diabetic mice to generate eNOS overexpressing eNOSTg/AK mice. Wild type, eNOSTg, AK and eNOSTg/AK mice were assessed for kidney function and blood glucose levels. Remarkably, overexpressing eNOSTg mice showed evidence of unpredicted glomerular injury with segmental mesangiolysis and occasional microaneurysms. Notably, in eNOSTg/AK mice overexpression of eNOS led to increased glomerular/endothelial injury that was associated with increased superoxide levels and renal dysfunction. Results indicate for the first time that overexpressing eNOS in endothelial cells cannot ameliorate diabetic lesions, but paradoxically leads to progression of nephropathy likely due to eNOS uncoupling and superoxide upsurge. This novel finding has a significant impact on current therapeutic strategies to improve endothelial function and prevent progression of diabetic renal disease. Further, the eNOSTg/AK model developed in this study has significant translational potentials for elucidating the underlying mechanism implicated in the deflected function of eNOS in diabetic nephropathy. 相似文献
10.
Babitha S Shin JH Nguyen DH Park SJ Reyes GA Caburian A Kim EK 《Archives of dermatological research》2011,303(3):211-216
In vitiligo, the active melanocytes in the epidermis are totally missing, whereas melanoblast cells in the outer root sheath
of hair follicles are not affected. In an attempt to find potent repigmenting agents for vitiligo therapy, pod extracts of
Cassia occidentalis was found to be effective in inducing differentiation and migration of mouse melanoblast cell line. Methanolic extract redissolved
in DMSO at 12.5 μg/ml was found to cause 3.5- to 3.8-fold melanin induction in melb-a melanoblast cells after 4 days in treatment
medium. In addition it induced the tyrosinase activity and altered melb-a cell morphology. Transwell migration assay showed
the potential of this herbal candidate to induce direct migration of treated cells. To the best of our knowledge, this is
the first report investigating the effect of Cassia occidentalis on the differentiation and migration of melanoblast cells. The findings of present study are significant in designing preclinical
and clinical studies on the efficacy of C. occidentalis as a stimulant for skin repigmentation in vitiligo. 相似文献