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排序方式: 共有326条查询结果,搜索用时 671 毫秒
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Yuji Okuyama Mitsuhiko Yamada Chikako Kondo Eisaku Satoh Shojiro Isomoto Takashi Shindo Yoshiyuki Horio Masafumi Kitakaze Masatsugu Hori Y. Kurachi 《Pflügers Archiv : European journal of physiology》1998,435(5):595-603
The effects of potassium channel opening drugs and intracellular nucleotides on the ATP-sensitive K+ (KATP) channel composed of SUR2A and Kir6.2 in HEK293T cells were examined using the patch-clamp technique. The SUR2A/Kir6.2 channel
was activated effectively by pinacidil, marginally by nicorandil but not by diazoxide. The pinacidil-activated channel currents
were inhibited by glibenclamide with a K
i value of 160 nM. Upon formation of inside-out (I-O) patches, spontaneous openings of the channels appeared, which were inhibited
by intracellular ATP (ATPi) equipotently in the presence and in the absence of intracellular Mg2+ (Mg2+
i). The channel activity ran-down gradually in I-O patches. The run-down channels could be reactivated by ATPi only in the presence of Mg2+
i. Uridine 5’-diphosphate (UDP) antagonized the ATPi-mediated inhibition of the channel activity before run-down. After run-down, UDP activated the channel without antagonizing
ATPi-mediated channel inhibition. Thus, the SUR2A/Kir6.2 reproduced the major properties of the native cardiac KATP channel well in terms of nucleotide regulation and pharmacology, and therefore can be a useful tool with which to elucidate
the molecular mechanisms characterizing the KATP channel.
Received: 24 October 1997 / Received after revision and accepted: 4 December 1997 相似文献
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Effects of nocturnal bright light on saliva melatonin, core body temperature and sleep propensity rhythms in human subjects 总被引:1,自引:0,他引:1
Kubota T Uchiyama M Suzuki H Shibui K Kim K Tan X Tagaya H Okawa M Inoue S 《Neuroscience research》2002,42(2):115-122
Nine healthy male volunteers (mean age of 24) participated in two experimental sessions of random crossover design: a bright light (5000 lux for 5 h from 00:00 to 05:00 h) session and a dim light (10 lux for 5 h from 00:00 to 05:00 h) session. Subsequently participants entered an ultra-short sleep-wake schedule for 26 h, in which a sleep-wake cycle consisting of 10-min sleep EEG recording on a bed and 20-min resting awake on a semi-upright chair were repeated. Saliva melatonin level and core body temperature was measured throughout the experiment. Bright light significantly delayed rhythms of melatonin secretion (01:58 h), core body temperature (01:12 h) and sleep propensity (02:00 h), compared as dim light session. Significant positive correlation was found between bright light-induced phase change in core body temperature and that in sleep propensity rhythm. Light-induced melatonin suppression significantly positively correlated with the phase change in core body temperature and that in sleep propensity rhythm. Assuming that light-induced melatonin suppression represents an acute impact of light on the circadian pacemaker, our results suggest that such an impact may be directly reflected in phase changes of sleep propensity and core body temperature rhythms rather than in melatonin rhythm. 相似文献
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Protein-Losing Enteropathy in Systemic Lupus Erythematosus 总被引:3,自引:0,他引:3
Shojiro Takagi M.D. Kazuo Oshimi M.D. Morito Sumiya M.D. Nobuyuki Gonda M.D. Shogo Kano M.D. Fumimaro Takaku M.D. 《The American journal of gastroenterology》1983,78(3):152-154
Although protein-losing enteropathy can be associated with a variety of disorders, only three cases have been described in association with systemic lupus erythematosus. In the case described herein, protein-losing enteropathy was the only clinical manifestation of systemic lupus erythematosus. Small intestinal biopsy revealed edema and mild mononuclear cell infiltration in lamina propria mucosae and no evidence of lymphangiectasia. X-ray studies of the gastrointestinal tract were normal. Protein-losing enteropathy responded to high-dose corticosteroid therapy. Protein-losing enteropathy should be suspected as a possible cause of unexplained hypoalbuminemia in systemic lupus erythematosus. 相似文献
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Nishi Takeki Fukui Kenji Matumoto Sari Takasu Shojiro Iwadate Kimiharu 《International journal of legal medicine》2022,136(2):513-518
International Journal of Legal Medicine - X-chromosomal short tandem repeats (X-STRs) are useful for the identification of absent single parents and complex blood relations. In the present study,... 相似文献
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An optimal therapeutic expression level is crucial for suicide gene therapy for hepatic metastatic cancer in mice 总被引:5,自引:0,他引:5
Terazaki Y Yano S Yuge K Nagano S Fukunaga M Guo ZS Komiya S Shirouzu K Kosai K 《Hepatology (Baltimore, Md.)》2003,37(1):155-163
The most serious problem in current gene therapy is discrepancies between experimental data and actual clinical outcomes, which may be due to insufficient analyses and/or inappropriate animal models. We have explored suicide gene therapy by using various clinically relevant animal models and doubt the clinical use of maximal suicide gene expression, which has been generally recommended. To explore this subject further, we studied what expression level of suicide gene and what promoter led to the maximal clinical benefit in the case of hepatic metastatic cancer in mice. Therapeutic and adverse side effects of 4 adenoviral vectors that express herpes simplex virus thymidine kinase (HSV-tk) under different promoters were scrupulously investigated in 2 mouse models of hepatic metastasis of gastric cancer that possess clinical characteristics. Surprisingly, increases in HSV-tk expression beyond a certain point, achieved by the Rous sarcoma virus long terminal repeat promoter, not only enhanced the adverse side effects of lethal hepatotoxicity and ganciclovir-independent cytotoxicity but also failed to further increase therapeutic potential. Moreover, the carcinoembryonic antigen (CEA) tumor-specific promoter, the therapeutic potential of which had been underestimated, was much more useful-even in the case of low CEA-producing cancer-than had been previously reported. In conclusion, the optimal therapeutic expression level of a suicide gene is a novel concept and a crucial factor for successful cancer gene therapy. The present results, which contradict those of previous studies, alert researchers about possible problems with ongoing and future clinical trials that lack this concept. 相似文献
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