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排序方式: 共有292条查询结果,搜索用时 31 毫秒
1.
Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphylococcus aureus. 总被引:2,自引:0,他引:2
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M Shimaoka M Yoh A Segawa Y Takarada K Yamamoto T Honda 《Journal of clinical microbiology》1994,32(8):1866-1869
A DNA hybridization method with an enzyme-labeled oligonucleotide probe (mecA-ELONP) was developed to detect the methicillin-resistant gene (mecA) in methicillin-resistant Staphylococcus aureus. For rapid identification, bacterial colonies were transferred from agar plates directly onto nylon membranes. Lysis of cells, denaturation of DNA, and hybridization were performed on the membranes. These procedures required only 3 h for completion. The results obtained by this test closely corresponded with those obtained by determining the MICs of oxacillin against S. aureus. The results of the mecA-ELONP also correlated well with those of a commercially available PCR test. Thus, mecA-ELONP proved to be a reliable and convenient method for the rapid identification of methicillin-resistant S. aureus, which could be useful in clinical microbiology laboratories. 相似文献
2.
The effects of resistance exercise combined with vascular occlusion on muscle function were investigated in highly trained
athletes. Elite rugby players (n=17) took part in an 8 week study of exercise training of the knee extensor muscles, in which low-intensity [about 50% of
one repetition maximum] exercise combined with an occlusion pressure of about 200 mmHg (LIO, n=6), low-intensity exercise without the occlusion (LI, n=6), and no exercise training (untrained control, n=5) were included. The exercise in the LI group was of the same intensity and amount as in the LIO group. The LIO group showed
a significantly larger increase in isokinetic knee extension torque than that in the other two groups (P<0.05) at all the velocities studied. On the other hand, no significant difference was seen between LI and the control group.
In the LIO group, the cross-sectional area of knee extensors increased significantly (P<0.01), suggesting that the increase in knee extension strength was mainly caused by muscle hypertrophy. The dynamic endurance
of knee extensors estimated from the decreases in mechanical work production and peak force after 50 repeated concentric contractions
was also improved after LIO, whereas no significant change was observed in the LI and control groups. The results indicated
that low-intensity resistance exercise causes, in almost fully trained athletes, increases in muscle size, strength and endurance,
when combined with vascular occlusion.
Electronic Publication 相似文献
3.
Gastric Acidity following Pancreaticogastrostomy with Pylorus-preserving Pancreaticoduodenectomy 总被引:4,自引:0,他引:4
Pancreaticogastrostomy (PG) has been reintroduced and employed occasionally as a useful alternative to pancreaticojejunostomy
(PJ) after Whipple resection or pylorus-preserving pancreaticoduodenectomy (PPPD). Although the physiologic alteration in
the stomach is important for the correlation between gastric and pancreatic functions, the actual intragastric pH profile
after PG is still unclear. This study was conducted to investigate the physiologic changes in gastric pH and serum gastrin
and secretin levels before and after PPPD reconstructed with PG (PPPD-PG) in humans. Twenty-four hour continuous intragastric
pH and serum gastrin and secretin levels in the fasting state were examined in 25 patients who had undergone PPPD-PG. No peptic
ulcer was detected after the operation. After PG, serum gastrin and secretin levels were unchanged. Twenty-four hour gastric
pH monitoring revealed two distinct patterns during the nocturnal period before the operation: patients with acid-type secretion
(n= 11) exhibited a persistent acid pH, whereas those with alkaline-type secretion (n= 14) had cyclic variations between an acid and an alkaline pH value. After PG, in both acid- and alkaline-type patients,
median pH and percentages of time that the gastric pH was less than 4 (% pH < 4) and more than 6 (% pH > 6) did not change,
and circadian pH patterns also remained unchanged. These results suggest that PPPD-PG has little influence on gastric acidity,
and the neurohumoral relation between the stomach, duodenum, and pancreas is preserved after PG. Therefore, physiologically,
PG can be recommended as a reconstructive procedure after PPPD. 相似文献
4.
Satoshi Daitoku Toshinori Yuasa Hiroshi Tsunenari Shigeho Maenohara Kazuharu Mine Yuichi Tamatsu Kazuyuki Shimada Chihaya Koriyama Mitsuru Ohishi Masahisa Horiuchi 《Environmental health and preventive medicine》2015,20(3):216-223
Objectives
Although carotid artery structural variations have been detected by ultrasound, their clinical significance is not fully understood. The objective of this study was to determine whether the angle between the common carotid artery (CCA) and the internal carotid artery (ICA), designated angle α, an ultrasound-detectable carotid artery structural variation, is related to carotid artery intima-media thickness (IMT), a surrogate marker for carotid atherosclerosis.Methods
As a cross-sectional study, we measured angle α in routine carotid artery ultrasounds from 176 subjects (130 men) with atherosclerotic disease/risk factors that attended Kouseiren Hospital in Kagoshima City, Japan between August 2007 and April 2009. We evaluated the correlation between the angle α and CCA- or ICA-IMT.Results
Angle α was weakly correlated with age but significantly correlated with ICA-IMT. The correlation was stronger in subjects with an ICA-IMT ≥ 0.5 mm than in those with an ICA-IMT < 0.5 mm (Right side r = 0.475 vs. 0.246, Left side r = 0.498 vs. 0.301, respectively). Upon multivariate logistic regression analysis, angle α and serum low-density lipoprotein cholesterol were independent explanatory variables for ICA-IMT.Conclusion
Angle α is related to ICA-IMT in subjects with atherosclerotic disease or risk factors in this study.Electronic supplementary material
The online version of this article (doi:10.1007/s12199-015-0453-7) contains supplementary material, which is available to authorized users. 相似文献5.
Chikao Iwai Hozuka Akita Nobuyuki Shiga Eiji Takai Yoshitomo Miyamoto Masakatsu Shimizu Hiroya Kawai Akira Takarada Teishi Kajiya Mitsuhiro Yokoyama 《Circulation journal》2002,66(8):723-728
Beta-1-adrenergic receptor (beta1-AR) blockers reduce both the incidence of sudden death and the ventricular volume in heart failure. In vitro, the Gly389 variant of beta1-AR mediates less adenylyl cyclase activities than the Arg389 variant, so Arg389Gly polymorphism was investigated with regard to the genesis, progression, or arrhythmogenesis of dilated cardiomyopathy (DCM). Allele and genotype frequencies of the Arg389Gly polymorphism were determined in 163 DCM patients and 157 age- and sex-matched controls. There were no differences in genotype and allele frequencies between patients and controls. Echocardiograms, left ventriculograms and 24h-Holter electrocardiograms were evaluated in the DCM patients and none of the clinical indices, other than ventricular tachycardia (VT), differed among the 3 genotypes. The Gly389 allele was more frequent in the VT(-) group than in the VT(+) group (0.46 vs 0.24, p=0.001). In univariate analysis, the odds ratio for VT in patients carrying 1 or 2 copies of the Gly389 allele was 0.29 ([95% confidence interval, 0.13-0.64], p=0.002), when compared with the Arg389 homozygotes. The Gly389 variant supressed the occurrence of VT in DCM, suggesting that this allele confers a decreased risk of sudden death. 相似文献
6.
Tomoki Tokuda Ryo Tsuruda Takuya Hara Haruki Kobayashi Katsufumi Tanaka Wataru Takarada Takeshi Kikutani Juan P. Hinestroza Joselito M. Razal Midori Takasaki 《Materials》2020,13(24)
Melt-electrospinning is an eco-friendly method for producing ultra-fine fibers without using any solvent. We prepared webs of poly(ethylene terephthalate) (PET) through melt-electrospinning using CO2 laser irradiation for heating. The PET webs comprised ultra-fine fibers of uniform diameter (average fiber diameter = 1.66 μm, coefficient of variation = 19%). The co-existence of fibers with high and low molecular orientation was confirmed through birefringence measurements. Although the level of high orientation corresponded to that of commercial highly oriented yarn, crystalline diffraction was not observed in the wide-angle X-ray diffraction (WAXD) analysis of the webs. The crystallinity of the webs was estimated using differential scanning calorimetry (DSC). The fibers with higher birefringence did not exhibit any cold crystallization peak. After annealing the web at 116 °C for 5 min, a further increase in the birefringence of the fibers with higher orientation was observed. The WAXD results revealed that the annealed webs showed crystalline diffraction peaks with the orientation of the c-axis along the fiber axis. In summary, the formation of fibers with a unique non-crystalline structure with extremely high orientation was confirmed. 相似文献
7.
8.
Yasushi Imamura Hirofumi Uto Yasunari Hiramine Kaori Hosoyamada Sho Ijuin Shiro Yoshifuku Hironori Miyahara Shigeho Maenohara Makoto Oketani Akio Ido Hirohito Tsubouchi 《Journal of gastroenterology》2014,49(10):1406-1413
Background
The prevalence of diabetes mellitus (DM) has been increasing. The present study was carried out to examine the relationship between this increase and fatty liver.Methods
Japanese participants who underwent regular health examinations in 1991, 1996, 2001, 2006, and 2011 were enrolled. Fatty liver was diagnosed using ultrasonography. DM was defined as requiring the use of medication for DM, having a fasting blood glucose level ≥126 mg/dl, or hemoglobin A1c level ≥6.5 %.Results
Logistic regression analysis on data from 11,235 participants (6,882 men and 4,271 women) in 2011 revealed that the association between fatty liver and DM was independent of age, body composition, and other confounders [odds ratio (OR) 1.97, 95 % confidence interval (95 % CI) 1.66–2.32 in men, and OR, 3.12; 95 % CI, 2.29–4.26 in women]. In 2006, 5,318 participants did not have DM and were able to be followed up in 2011. Fatty liver in 2006 was an independent predictor of DM in 2011 [OR 1.73 (95 % CI 1.20–2.50) in men, 4.13 (2.16–8.10) in women]. The prevalence of DM increased significantly during the 20-year period examined among both men (6.0, 8.9, 10.0, 10.8, 12.0 %, P < 0.001) and women (3.3, 4.5, 4.2, 4.1, 5.1 %, P = 0.004), accompanied with an increased prevalence of fatty liver among both men (10.8, 26.3, 33.8, 36.7, and 38.0 %, P < 0.001) and women (6.5, 16.7, 22.2, 21.3, and 20.8 %, P < 0.001).Conclusion
Fatty liver independently predicts both present and future DM. Fatty liver may play an important role in the recent increases in the prevalence of DM. 相似文献9.
Mana Hatanaka Yoichi Hatamoto Eri Tajiri Naoyuki Matsumoto Shigeho Tanaka Eiichi Yoshimura 《Nutrients》2022,14(2)
Recent studies have reported that meal timing may play an important role in weight regulation, however it is unknown whether the timing of meals is related to the amount of weight loss. This study aimed to examine the relationship between indices of meal timing and weight loss during weight loss intervention in adults. A 12-week weight loss support program was conducted for 97 adults (age: 47.6 ± 8.3 years, BMI: 25.4 ± 3.7 kg/m2). After the program, body weight decreased by −3.0 ± 2.7%. Only the start of the eating window was positively correlated with the weight change rate in both sexes (men: r = 0.321, p = 0.022; women: r = 0.360, p = 0.014). The participants were divided into two groups based on the start of the eating window as follows: the early group (6:48 ± 0:21 AM) and the late group (8:11 ± 1:05 AM). The weight loss rate in the early group was significantly higher (−3.8 ± 2.7%) than that in the late group (−2.2 ± 2.5%). The present results showed that the start of the early eating window was associated with weight loss and suggested paying attention to meal timing when doing weight loss. 相似文献
10.