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YOSHIAKI SONODA TAKAFUMI KIMURA YOSHIKAZU OHMIZONO HIDEAKI SAKABE SHIGEATSU TANIMUKAI SHOUHEI YOKOTA STEVEN C. CLARK & TATSUO ABE 《British journal of haematology》1997,96(4):781-789
We studied the interaction of interleukin (IL)-4 and other burst-promoting activity (BPA) factors, such as IL-3, granulocyte/macrophage colony-stimulating factor (GM-CSF), IL-9 and stem cell factor (SCF), on erythroid burst-forming unit (BFU-E) and erythrocyte-containing mixed (CFU-Mix) colony formation in serum-free culture. IL-4 alone did not support mixed colony formation in the presence of erythropoietin (Epo). However, IL-4 showed weak but significant BPA when peripheral blood (PB)-derived CD34+c-kitlow cells were used as the target population. The BPA of IL-4 was much weaker than that of IL-3, which exerted the most potent activity, as previously reported. When CD34+c-kithigh cells were used as the target, four factors known to have BPA, IL-3, GM-CSF, IL-9 and SCF, could express BPA. In contrast, IL-4 alone failed to support erythroid burst formation. Interestingly, IL-4 showed a remarkable enhancing effect with SCF in promoting the development of erythroid burst and erythrocyte-containing mixed colonies from CD34+c-kitlow and CD34+c-kithigh cells. Delayed addition of SCF + Epo or IL-4+Epo to the cultures initiated with either IL-4 or SCF alone clearly demonstrated that SCF was a survival factor for both BFU-E and CFU-Mix progenitors. In contrast, the survival effect of IL-4 was much weaker than that of SCF, and appeared to be more important for progenitors derived from CD34+c-kitlow cells than for those derived from CD34+c-kithigh cells. It was recently reported that CD34+c-kitlow cells represent a more primitive population than CD34+c-kithigh cells. Taken together, these results suggest that IL-4 helps to recruit primitive progenitor cells in the presence of SCF. 相似文献
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