全文获取类型
收费全文 | 2167篇 |
免费 | 238篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 53篇 |
儿科学 | 84篇 |
妇产科学 | 25篇 |
基础医学 | 340篇 |
口腔科学 | 22篇 |
临床医学 | 279篇 |
内科学 | 457篇 |
皮肤病学 | 47篇 |
神经病学 | 160篇 |
特种医学 | 90篇 |
外科学 | 363篇 |
综合类 | 104篇 |
预防医学 | 171篇 |
眼科学 | 40篇 |
药学 | 90篇 |
中国医学 | 1篇 |
肿瘤学 | 85篇 |
出版年
2023年 | 16篇 |
2022年 | 15篇 |
2021年 | 30篇 |
2020年 | 21篇 |
2019年 | 35篇 |
2018年 | 39篇 |
2017年 | 33篇 |
2016年 | 24篇 |
2015年 | 26篇 |
2014年 | 42篇 |
2013年 | 61篇 |
2012年 | 117篇 |
2011年 | 96篇 |
2010年 | 60篇 |
2009年 | 77篇 |
2008年 | 105篇 |
2007年 | 122篇 |
2006年 | 86篇 |
2005年 | 85篇 |
2004年 | 108篇 |
2003年 | 112篇 |
2002年 | 106篇 |
2001年 | 58篇 |
2000年 | 83篇 |
1999年 | 75篇 |
1998年 | 35篇 |
1997年 | 31篇 |
1996年 | 24篇 |
1995年 | 22篇 |
1994年 | 22篇 |
1993年 | 14篇 |
1992年 | 33篇 |
1991年 | 43篇 |
1990年 | 39篇 |
1989年 | 29篇 |
1988年 | 26篇 |
1987年 | 44篇 |
1986年 | 33篇 |
1985年 | 42篇 |
1984年 | 27篇 |
1983年 | 30篇 |
1982年 | 14篇 |
1979年 | 29篇 |
1978年 | 13篇 |
1977年 | 17篇 |
1974年 | 17篇 |
1972年 | 12篇 |
1970年 | 27篇 |
1969年 | 22篇 |
1968年 | 18篇 |
排序方式: 共有2411条查询结果,搜索用时 15 毫秒
1.
Although bladder function is thought to be unaffected in Duchenne muscular dystrophy, 46/88 boys interviewed had urinary problems. Nine underwent video urodynamics, showing in eight a small capacity, hyperreflexic bladder, and in the ninth (post spinal surgery) hyperreflexia and detrusor sphincter dyssynergia. Urinary dysfunction is a treatable feature of DMD. 相似文献
2.
3.
Bayrak S; Holmdahl R; Travers P; Lauster R; Hesse M; Dolling R; Mitchison NA 《International immunology》1997,9(11):1687-1699
Type II collagen (CII) is of immunological interest because of its
repetitive structure and properties as an autoantigen. The mouse gene has
recently been cloned, thus enabling T cell-defined epitopes to be
identified. Multiple novel epitopes on mouse CII are here detected in the
autoreactive T cell response. The major response is directed to an epitope
with residues 707-721 located on the CB10 fragment. Some 25 other epitopes
are also recognized, including the autologous homologue of the 256-270
epitope which dominates in the response to foreign collagen. The cells
reactive with mouse collagen peptides were of Th1 type, as judged by
release of IFN-gamma. No significant reactivity was detected to mouse CII
peptides during ongoing disease. Alignment of the mouse epitopes revealed a
sequence motif with characteristic side chains at residues P1, P4 and P7,
and to a lesser extent at P5, within a nonamer core sequence. Binding of
these epitopes was simulated in a computer model of the I-Aq molecule,
where peptides with anchor residues at P1, P4 and P7 were indeed found to
fit the binding groove best. The spacing of pockets and the fine structure
of the binding surface of the I-Aq molecule meshes with the repetitive
structure of the collagen (X-Y-Gly), thus providing a likely explanation
for the occurrence of multiple epitopes. Comparison with human DR binding
motifs showed that the I-Aq motif resembles most closely that of the DR4
subtypes which predispose for rheumatoid arthritis.
相似文献
4.
5.
The acromioclavicular joint is a potential source of pain in the shoulder. There are a variety of disorders that can affect this joint, including distal clavicle osteolysis, posttraumatic arthritis, osteoarthritis, and rheumatoid arthritis. Nonoperative treatment for this condition with nonsteroidal medication and activity modification can alleviate the pain. When conservative treatment is exhausted, surgical resection of the distal clavicle is often necessary. Arthroscopic resection of the distal clavicle preserves the acromioclavicular ligaments to prevent postoperative distal clavicle instability. The procedure is performed in either the beach chair or lateral position and requires the use of a shaver, electrocautery, and a burr for soft tissue and debridement and bone resection. 相似文献
6.
7.
Debra L Ellies Beth Viviano John McCarthy Jean-Philippe Rey Nobue Itasaki Scott Saunders Robb Krumlauf 《Journal of bone and mineral research》2006,21(11):1738-1749
We compared and contrasted the mechanism of action for the cysteine knot protein subfamily, Wise and Sost (Sclerostin). Our data suggest that functional interactions between Sost or Wise and LRP5/LRP6 have the potential to regulate bone deposition by modulating the Wnt pathway. INTRODUCTION: The human disease sclerosteosis exhibits an increase in bone mass thought to be caused by hyperactive osteoblasts. Sclerostin, SOST, the gene affected in this disease, has been postulated to exert its activity by functioning as a BMP antagonist. However, recent evidence indicates that SOST is highly related to Wise, which can also modulate the Wnt pathway by binding to LRP5 and LRP6. MATERIALS AND METHODS: For this study, we used cell culture to test the BMP and Wnt activity function of both Wise and Sost. In addition, we used Xenopus in vivo Wnt assays along with Xenopus in vitro Wnt assays to support our cell culture results. Epitope tagged cell supernatants containing either Sost or soluble mutant or wildtype LRP5/LRP6 were used for immunoprecipitation. Sost immunoprecipitation results were confirmed in vivo using cell culture. Finally, to support our in vitro data, we co-localized Sost, Wise, LRP5, and LRP6 in mouse long bone sections. Results: In this study, we report in vitro and in vivo evidence to show that Sost physically interacts with Lrp5 and Lrp6 and inhibits the canonical Wnt signaling pathway. Furthermore, using in vitro and in vivo assays, we showed that a variant of LRP5 (LRP5(G171V)) known to cause the human high bone mass (HBM) trait and a homologous change in LRP6 (LRP6(G158V)) abolished protein interactions with Sost. We used variants of Sost amino acids to further identify the contact points between Sost and LRP6. In Xenopus and mammalian cell culture assays, we showed that SOST is able to attenuate Wnt signaling and that this attenuation can be rescued by the addition of alpha-Sost antibodies or by the introduction of single amino acid substitution that alter its binding to LRP6. Sost differs from Wise in that it is unable to stimulate Wnt signaling. Using immunohistochemistry, we found that Sost and Wise are co-localized to osteoblasts, along with LRP5 and LRP6. CONCLUSIONS: Our data suggest that functional interactions between Sost or Wise and LRPs have the potential to regulate bone deposition by modulating Wnt signaling. 相似文献
8.
9.
Gil Bellis Marie-Hélène Cazes Alain Parant Maryse Gaimard Cécile Travers Evelyne Le Roux Sophie Ravilly Gilles Rault 《Journal of cystic fibrosis》2007,6(3):179-186
BACKGROUND: In 1992 France set up a national cystic fibrosis observatory (Observatoire national de la mucoviscidose, ONM) to monitor the state of health of patients on an annual basis. Using the ONM data, this study estimates the main indicators for life expectancy and assesses the total number of cystic fibrosis patients. METHODS: The data for the years 1994 to 2003 are divided into 3-year periods. Life tables are drawn up for these periods, from which mean and median lengths of life are determined. Using the most recent life table, the number of births in 2003 and the incidence of the disease, the total population of patients can be estimated, assuming a stationary population. RESULTS: In 2001-2003, life expectancy at birth of patients registered with the ONM was 39.1 years and median length of life was 36.4 years. These results, substantially better than those of 1994-1996, are linked to improved conditions of patient inclusion in the ONM database, to improvements in their healthcare, but also to the limitations of the life tables. Based on the 2003 data, the total theoretical number of patients is 6490, and coverage by the ONM database is thus 63.2%. CONCLUSIONS: These provisional results demonstrate the need to convert the ONM observatory into a registry providing exhaustive coverage of all patients. 相似文献
10.