全文获取类型
收费全文 | 911篇 |
免费 | 45篇 |
国内免费 | 10篇 |
专业分类
耳鼻咽喉 | 9篇 |
儿科学 | 30篇 |
妇产科学 | 25篇 |
基础医学 | 96篇 |
口腔科学 | 7篇 |
临床医学 | 57篇 |
内科学 | 152篇 |
皮肤病学 | 18篇 |
神经病学 | 47篇 |
特种医学 | 26篇 |
外科学 | 208篇 |
综合类 | 18篇 |
一般理论 | 1篇 |
预防医学 | 46篇 |
眼科学 | 13篇 |
药学 | 83篇 |
中国医学 | 5篇 |
肿瘤学 | 125篇 |
出版年
2023年 | 5篇 |
2022年 | 8篇 |
2021年 | 20篇 |
2020年 | 19篇 |
2019年 | 25篇 |
2018年 | 25篇 |
2017年 | 19篇 |
2016年 | 23篇 |
2015年 | 29篇 |
2014年 | 30篇 |
2013年 | 40篇 |
2012年 | 58篇 |
2011年 | 78篇 |
2010年 | 43篇 |
2009年 | 33篇 |
2008年 | 62篇 |
2007年 | 50篇 |
2006年 | 50篇 |
2005年 | 39篇 |
2004年 | 32篇 |
2003年 | 29篇 |
2002年 | 37篇 |
2001年 | 28篇 |
2000年 | 25篇 |
1999年 | 27篇 |
1998年 | 17篇 |
1997年 | 5篇 |
1996年 | 5篇 |
1995年 | 4篇 |
1994年 | 7篇 |
1993年 | 3篇 |
1992年 | 8篇 |
1991年 | 13篇 |
1990年 | 3篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1987年 | 5篇 |
1986年 | 2篇 |
1985年 | 5篇 |
1984年 | 3篇 |
1980年 | 4篇 |
1979年 | 4篇 |
1978年 | 2篇 |
1977年 | 5篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1971年 | 4篇 |
排序方式: 共有966条查询结果,搜索用时 15 毫秒
1.
2.
3.
Antibody to a 145-kilodalton outer membrane protein has bactericidal activity and protective activity against experimental bacteremia caused by a Brazilian purpuric fever isolate of Haemophilus influenzae biogroup aegyptius. The Brazilian Purpuric Fever Study Group. 总被引:2,自引:2,他引:0 下载免费PDF全文
The immunologic basis for protection against Brazilian purpuric fever, a septicemic infection associated with Haemophilus influenzae biogroup aegyptius bacteremia, is unknown. Passive immunization of infant rats with antiserum to whole bacterial cells of the homologous strain protects them from experimental bacteremia following bacterial challenge. In immunoblotting, antibody to a 145-kDa protein (P145) was present in protective antisera but not in nonprotective antisera. As judged by analysis of the antibodies eluted from whole bacterial cells and the agglutination of bacteria by antisera to P145, this protein is surface exposed. We prepared monospecific rat antisera to this protein by three methods: (i) immunization with whole bacterial cells and absorption with a Brazilian purpuric fever strain not expressing P145, (ii) immunization with gel-purified P145, and (iii) immunization with a P145-expressing transformant of a laboratory H. influenzae strain expressing this protein and absorption of the antiserum with the laboratory H. influenzae strain. These antisera had low antilipooligosaccharide antibody titers, were reactive only with P145, and had bactericidal activity in vitro. Following passive immunization, these antisera partially protected infant rats from bacteremia resulting from intraperitoneal challenge with bacteria. As assessed by immunoblotting, pooled adult human sera contained antibodies reactive with P145. Antibody to P145 may contribute to protection against Brazilian purpuric fever. 相似文献
4.
EMILIO SIENDONES YOLANDA JIMÉNEZ-GÓMEZ JOSÉ LUÍS MONTERO CONSUELO GÓMEZ-DÍAZ JOSÉ MANUEL VILLALBA JORDI MUNTANÉ 《Journal of gastroenterology and hepatology》2006,20(1):108-116
Background and Aim: PGE1 reduces in vivo and in vitro D-galactosamine (D-GalN)-induced cell death in hepatocytes. The present study was undertaken to elucidate the intracellular pathway by which D-GalN induces cell death in cultured hepatocytes. In addition, we evaluated if PGE1 was able to modulate different parameters related to D-GalN-induced apoptosis in cultured rat hepatocytes.
Methods: Hepatocytes were isolated from male Wistar rats (225–275 g) by the classical collagenase procedure. PGE1 (1 µM) was administered 2 h before D-GalN (5 mM) in primary culture of rat hepatocytes. Apoptosis was determined by DNA fragmentation and caspase-3, -6, -8 and -9 activation in hepatocytes. Caspase activation was evaluated by the detection of the related cleaved product and its associated activity. Cell necrosis was determined by the measurement of lactate dehydrogenase (LDH) activity in culture medium. To elucidate the role of mitochondria, we measured neutral (nSMase) and acid (aSMase) sphingomyelinase, as well as the expression of cytochrome c in mitochondria and cytoplasm fractions from D-GalN treated hepatocytes.
Results: D-GalN induced caspase-3 activation and DNA fragmentation in hepatocytes. This apoptotic response was not associated with the activation of caspase-6, -8 or -9. The use of specific inhibitors confirmed that only caspase-3 was involved in D-GalN-induced apoptosis. D-GalN did not modify nSMase and aSMase activities, nor mitochondrial cytochrome c release in hepatocytes.
Conclusions: D-GalN induced apoptosis through caspase-3 activation but without modification of the activity of caspase-6, -8, -9, SMases or cytochrome c release. PGE1 appears to prevent D-GalN-induced apoptosis by a mitochondria-independent mechanism. 相似文献
Methods: Hepatocytes were isolated from male Wistar rats (225–275 g) by the classical collagenase procedure. PGE
Results: D-GalN induced caspase-3 activation and DNA fragmentation in hepatocytes. This apoptotic response was not associated with the activation of caspase-6, -8 or -9. The use of specific inhibitors confirmed that only caspase-3 was involved in D-GalN-induced apoptosis. D-GalN did not modify nSMase and aSMase activities, nor mitochondrial cytochrome c release in hepatocytes.
Conclusions: D-GalN induced apoptosis through caspase-3 activation but without modification of the activity of caspase-6, -8, -9, SMases or cytochrome c release. PGE
5.
J. N. Banks S. J. Cox B. J. Northway R. H. Rizvi 《Food and Agricultural Immunology》1994,6(3):321-327
Monoclonal antibodies (MAbs) were raised against Penicillium aurantiogriseum var. melanoconidium. Cross‐reactivity studies were carried out against 12 ‘field’ and 27 ‘storage’ fungi. Two MAbs (MAbs 32 and 37) reacted with all of the fungi tested and a third (MAb 38) with 38 out of the 39 fungi (although weakly with some). Monoclonal antibodies 32 and 37, but not MAb 38, were found to react to a degree with ‘clean’ barley. Enzyme‐linked immunosorbent assay (ELISA) using MAb 38 and extracts of barley inoculated with the homologous antigen showed a clear relationship between absorbance and amount of fungal growth. It is suggested that these MAbs could be used in a broad‐spectrum assay to detect fungi of significance to the quality of foods and feeds. 相似文献
6.
7.
Conclusion From the above consideration we conclude that the stature of the boys and girls in all the age groups grew more rapidly than
the other body segments under consideration. On the other hand, the arm girth grew very slowly. The mean values were progressively
higher while the rate of growth and growth velocities were different in different age groups. The mean values of the lower
extremity and biacromial indices were nearly constant in all the age groups which showed relatively equal growth of the stature
in relation to lower extremity and shoulder breadth.
From the Department of Anthropology, Lucknow University, Lucknow-226001. 相似文献
8.
Muhammad Jamshaid Samia Yousuf Nadeem Irfan Bukhari Amir Ali Rizvi 《European journal of drug metabolism and pharmacokinetics》2003,28(1):1-6
Three commercially available brands of amikacin were investigated in a parallel study design for the assessment of comparative pharmacokinetics in pediatric oncology patients with chemotherapy-induced neutropenic febrile episode. Amikacin concentration in serum samples was determined by fluorescence polarization immunoassay method using Abbott TDx system. Computer software, PK II was used for computation of pharmacokinetic parameters of amikacin. The serum concentration of all brands nonsignificantly (p > 0.05) varied at all time points, except at 1 and 2 hrs post dosing. At 1 hr post dosing, the serum concentration of brand II varied from rest of two brands. Whereas at 2 hr following I/V infusion, brands II and I were statistically different. Highest serum concentration of 38.69 +/- 1.45 microg/ml was observed in case of brand III while brands I and II showed lower but not significantly different serum concentration values, i.e., 36.30 +/- 1.65 and 37.89 +/- 1.32 microg/ml, respectively when compared with brand I. The other pharmacokinetic parameters of 3 brands found to have non-significant difference (P < 0.05) except, t(1/2)alpha and Cl of brands I and II that deviated statistically significant (p < 0.01). The relative bioavailability of brand II and III as compared with brand I, considered as standard 86.17 and 96.86%, respectively falls within the accepted limits of +/- 20% required for the bioequivalence of any two brands. Based upon findings of the present study, all these brands may be used interchangeably in oncology patients. Further studies, however are needed to determine whether the statistically elevated Cl value in brand II is of any clinical significance. 相似文献
9.
10.