Cross-protection conferred by immunization with an rOmpH-based intranasal fowl cholera vaccine

A previous study demonstrated that a recombinant outer membrane protein H (rOmpH)-based intranasal fowl cholera vaccine elicited efficient homologous protection against the Pasteurella multocida strain X-73 (A:1) in chickens. The present study aimed to determine the cross-protectivity against heterologous P. multocida strains. The rOmpH was purified via electroelution and formulated with two kinds of adjuvants. The vaccine formulations in a total volume of 100?µl were 100?µg rOmpH with 3?µg of Escherichia coli enterotoxin B or 10?µg of CpG ODN2007. Chickens were assigned to three experimental groups depending on bacterial strain challenge exposure as well as three control groups. The chickens were immunized intranasally three times at three-week intervals. Challenge exposures were conducted by inoculation with homologous strain X-73 or heterologous strains P-1059 (A:3) or P-1662 (A:4) at four weeks after the final immunization. The specific antibody against rOmpH was produced in vaccinated birds. Sera IgY and secretory IgA antibody titres were significantly increased (P?P?P. multocida strains ranged from 70% to 100%. There was no significant difference in protection between two kinds of adjuvants in vaccine formulations. Statistical analysis indicated no significant differences in protection among avian P. multocida strains challenge exposure. We conclude that an in-house rOmpH-based intranasal fowl cholera vaccine produced efficient cross-protectivity against heterologous strains of P. multocida.     

Activity of quinones from teak (Tectona grandis) on fungal cell wall stress

Teak ( Tectona grandis L.f., Verbenaceae) sawdust extract inhibited the growth of Aspergillus niger. Centrifugal partition chromatography was used to isolate the active compounds. By (1)H-NMR the active compounds were identified as deoxylapachol and tectoquinone. Two A. niger transgenic strains which show induction of 1,3 -alpha-D-glucan synthase were used as a cell wall damage model. The result showed that deoxylapachol from T. grandis extract induced fungal cell wall stress.     

The retrospective identification and molecular epidemiology of porcine circovirus type 3 (PCV3) in swine in Thailand from 2006 to 2017

Porcine circovirus type 3 (PCV3) has recently been detected in pigs worldwide, with similar clinical manifestations to porcine circovirus‐associated disease (PCVAD) from porcine circovirus type 2 (PCV2) infection. Here, we report the identification and molecular epidemiology of PCV3 in swine in Thailand from clinical samples retrieved from 2006 to 2017. The epidemiological data revealed co‐infection with PCV2, PRRSV, and PCV2/PRRSV was common in our samples. Circulating PCV3 from this study shared a high similarity of nucleotide and deduced amino acid sequences of the partial capsid gene (96.7%–100% and 96.7%–100% respectively), indicated the genetic stability of PCV3 in Thailand. Phylogenetic analysis based on the capsid gene revealed scatter clustering with current PCV3 having no relation to the geographical origin of the virus strains. In this retrospective study, results have demonstrated that PCV3 has spread extensively within Thai swine from as early as 2006 and may also be involved in PRDC and PCVAD.     

Chinese-like Strain of Porcine Epidemic Diarrhea Virus, Thailand

Since late 2007, several outbreaks of porcine epidemic diarrhea virus (PEDV) infection have emerged in Thailand. Phylogenetic analysis places all Thai PEDV isolates during the outbreaks in the same clade as the Chinese strain JS-2004-2. This new genotype PEDV is prevailing and currently causing sporadic outbreaks in Thailand.     

Looking beyond the audiogram in ototoxicity associated with platinum-based chemotherapy

Cancer Chemotherapy and Pharmacology - Ototoxicity associated with platinum-based chemotherapy is highly prevalent and can cause detrimental consequences among cancer survivors. In this article, we...     

Experimental Investigation of Additive Manufacturing Using a Hot-Wire Plasma Welding Process on Titanium Parts

In this paper, we propose hot-wire plasma welding, a combination of the plasma welding (PAW) process and the hot-wire process in the additive manufacturing (AM) process. Generally, in plasma welding for AM processes, the deposit grain size increases, and the hardness decreases as the wall height increases. The coarse microstructure, along with the large grain size, corresponds to an increase in deposit temperature, which leads to poorer mechanical properties. At the same time, the hot-wire laser process seems to contain an overly high interstitial amount of oxygen and nitrogen. With an increasing emphasis on sustainability, the hot-wire plasma welding process offers significant advantages: deeper and narrow penetration than the cold-wire plasma welding, improved design flexibility, large deposition rates, and low dilution percentages. Thus, the hot-wire plasma welding process was investigated in this work. The wire used in the welding process was a titanium American Welding Society (AMS) 4951F (Grade 2) welding wire (diameter 1.6 mm), in which the welding was recorded in real time with a charge-coupled device camera (CCD camera). We studied three parameters of the hot-wire plasma welding process: (1) the welding speed, (2) wire current, and (3) wire feeding speed. The mechanical and physical properties (porosity, Vickers hardness, microstructure, and tensile strength) were examined. It was found that the number of layers, the length and width of the molten pool, and the width of the deposited bead increased, while the height of the layer increased, and the hot-wire current played an important role in the deposition. In addition, these results were benchmarked against specimens created by a hot-wire plasma welding/wire-based additive manufacturing process with an intention to develop the hot-wire PAW process as a potential alternative in the additive manufacturing industry.     

Simplify pulpectomy in primary molars with a single-file reciprocating system: a randomized controlled clinical trial

To compare the clinical use of a single-file NiTi reciprocal system (sNiTi) to that of stainless steel hand file (SSH) instrumentation in primary tooth pul