全文获取类型
收费全文 | 135篇 |
免费 | 24篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 8篇 |
妇产科学 | 2篇 |
基础医学 | 21篇 |
口腔科学 | 9篇 |
临床医学 | 13篇 |
内科学 | 50篇 |
皮肤病学 | 1篇 |
神经病学 | 2篇 |
特种医学 | 3篇 |
外科学 | 20篇 |
综合类 | 2篇 |
预防医学 | 3篇 |
药学 | 17篇 |
肿瘤学 | 8篇 |
出版年
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 11篇 |
2020年 | 6篇 |
2019年 | 12篇 |
2018年 | 3篇 |
2017年 | 10篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 8篇 |
2013年 | 7篇 |
2012年 | 13篇 |
2011年 | 12篇 |
2010年 | 4篇 |
2009年 | 8篇 |
2008年 | 3篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 1篇 |
2004年 | 2篇 |
2003年 | 7篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 7篇 |
1999年 | 6篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有160条查询结果,搜索用时 218 毫秒
1.
2.
beta-glucuronidase, cathepsin D, acid and alkaline phosphatases were studied in rhesus monkey endometrium during the menstrual cycle (day -6 to day +10) and pre-implantation stages (day +3 to day +6) of gestation, with day 0 considered as the day of ovulation. Acid hydrolases exhibited low levels in proliferative phase endometria followed by their gradual rise in the secretory phase of the menstrual cycle. Despite no shifts in the levels of serum progesterone and estradiol-17 beta, the pre-implantation period was, however, associated with distinct changes in enzyme profiles characterized by lower absolute levels (P less than 0.05) of acid phosphatase and beta-glucuronidase on days 3 to 6 of gestation, whereas cathepsin D activity declined significantly (P less than 0.05) on days 5 and 6. Alkaline phosphatase showed a characteristic rise during the pre-ovulatory period with a gradual lowering of its level in post-ovulatory phase endometria of a non-fertile cycle; in contrast, during early gestation, alkaline phosphatase activity showed a marked elevation (P less than 0.05) on days 5 and 6 of gestation. The significance of these findings is discussed. 相似文献
3.
Fereshteh Shafiei Paria Dehghanian Mahtab Memarpour Mohammad Sabeti 《Clinical oral investigations》2020,24(2):711-718
To evaluate the effect of Er:YAG etching on topography, microstructure, compressive strength, and shear bond strength (SBS) of All-Bond Universal adhesive 相似文献
4.
5.
Residual soil phosphorus as the missing piece in the global phosphorus crisis puzzle 总被引:10,自引:0,他引:10
Sattari SZ Bouwman AF Giller KE van Ittersum MK 《Proceedings of the National Academy of Sciences of the United States of America》2012,109(16):6348-6353
Phosphorus (P) is a finite and dwindling resource. Debate focuses on current production and use of phosphate rock rather than on the amounts of P required in the future to feed the world. We applied a two-pool soil P model to reproduce historical continental crop P uptake as a function of P inputs from fertilizer and manure and to estimate P requirements for crop production in 2050. The key feature is the consideration of the role of residual soil P in crop production. Model simulations closely fit historical P uptake for all continents. Cumulative inputs of P fertilizer and manure for the period 1965-2007 in Europe (1,115 kg . ha(-1) of cropland) grossly exceeded the cumulative P uptake by crops (360 kg ha(-1)). Since the 1980s in much of Europe, P application rates have been reduced, and uptake continues to increase due to the supply of plant-available P from residual soil P pool. We estimate that between 2008 and 2050 a global cumulative P application of 700-790 kg . ha(-1) of cropland (in total 1,070-1,200 teragrams P) is required to achieve crop production according to the various Millennium Ecosystem Assessment scenarios [Alcamo J, Van Vuuren D, Cramer W (2006) Ecosystems and Human Well-Being: Scenarios, Vol 2, pp 279-354]. We estimate that average global P fertilizer use must change from the current 17.8 to 16.8-20.8 teragrams per year in 2050, which is up to 50% less than other estimates in the literature that ignore the role of residual soil P. 相似文献
6.
Spear GT Kersh E Guenthner P Vishwanathan SA Gilbert D Zariffard MR Mirmonsef P Landay A Zheng L Gillevet P 《AIDS research and human retroviruses》2012,28(10):1244-1249
Abstract Vaginal bacterial communities play an important role in human health and have been shown to influence HIV infection. Pigtailed macaques (Macaca nemestrina) are used as an animal model of HIV vaginal infection of women. Since the bacterial microbiota could influence retrovirus infection of pigtailed macaques, the genital microbiota in 10 cycling macaques was determined by pyrosequencing. The microbiota of all macaques was polymicrobial with a median of 13 distinct genera. Strikingly, the genera Sneathia and Fusobacterium, both in the phylum Fusobacteria, accounted for 18.9% and 13.3% of sequences while the next most frequent were Prevotella (5.6%), Porphyromonas (4.1%), Atopobium (3.6%), and Parvimonas (2.6%). Sequences corresponding to Lactobacillus comprised only 2.2% of sequences on average and were essentially all L. amylovorus. Longitudinal sampling of the 10 macaques over an 8-week period, which spanned at least one full ovulatory cycle, showed a generally stable presence of the major types of bacteria with some exceptions. These studies show that the microbiota of the pigtailed macaques is substantially dissimilar to that found in most healthy humans, where the genital microbiota is usually dominated by Lactobacillus sp. The polymicrobial makeup of the macaque bacterial populations, the paucity of lactobacilli, and the specific types of bacteria present suggest that the pigtailed macaque microbiota could influence vaginal retrovirus infection. 相似文献
7.
Estrogenic responses in estrogen receptor-α deficient mice reveal a distinct estrogen signaling pathway
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
8.
Preimplantation embryo development in vitro: cooperative interactions among embryos and role of growth factors. 总被引:11,自引:5,他引:11
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
B C Paria S K Dey 《Proceedings of the National Academy of Sciences of the United States of America》1990,87(12):4756-4760
We have established a model that shows cooperative interaction among preimplantation embryos and the role of growth factors on their development and growth. Two-cell mouse embryos cultured singly in 25-microliters microdrops had inferior development to blastocysts and lower cell numbers per blastocyst compared with those cultured in groups of 5 or 10. The inferior development of singly cultured embryos was markedly improved by addition of epidermal growth factor (EGF) or transforming growth factor alpha or beta 1 (TGF-alpha or TGF-beta 1) to the culture medium. The stage of embryonic development, primarily affected by these treatments, was between eight-cell/morula and blastocyst. Furthermore, blastocysts developed from eight-cell embryos cultured in groups or singly in the presence of EGF showed a higher incidence of zona hatching compared with those cultured singly in the absence of EGF. Detection of EGF receptors on the embryonic cell surface at eight-cell/morula and blastocyst stages suggests beneficial effects of EGF or TGF-alpha on preimplantation embryo development and blastocyst functions. Insulin-like growth factor I (IGF-I) had no influence on embryo development. To further document the cooperative interactions among embryos, the volume of the culture medium was doubled to 50 microliters. This increase in culture volume was even more detrimental to the development of singly cultured embryos. However, this detrimental effect was significantly reversed by EGF and reversed even more markedly by a combination of EGF and TGF-beta 1 but not by TGF-beta 1 alone. Although TGF-beta 1 plus IGF-I caused a modest improvement of embryo development, the response was not as great as shown by EGF alone. Furthermore, IGF-I had no additive effect on EGF-induced embryonic development. The study presents clear evidence that specific growth factors of embryonic and/or reproductive tract origin participate in preimplantation embryo development and blastocyst functions in an autocrine/paracrine manner. 相似文献
9.
Differential G protein-coupled cannabinoid receptor signaling by anandamide directs blastocyst activation for implantation 总被引:7,自引:0,他引:7
下载免费PDF全文
![点击此处可从《Proceedings of the National Academy of Sciences of the United States of America》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Wang H Matsumoto H Guo Y Paria BC Roberts RL Dey SK 《Proceedings of the National Academy of Sciences of the United States of America》2003,100(25):14914-14919
Mammalian fertility absolutely depends on synchronized development of the blastocyst to the stage when it is competent to implant, and the uterus to the stage when it is receptive to implantation. However, the molecular basis for the reciprocal interaction between the embryo and the uterus remains largely unexplored. One potentially important mechanism involves signaling between an evolutionarily conserved G protein-coupled protein cannabinoid receptor, CB1, that is expressed at high levels on the surface of the trophectoderm and anandamide (N-arachi-donoylethanolamine), an endocannabinoid ligand found to be produced at higher levels by the uterus before implantation and then down-regulated at the time of implantation. Using genetic, pharmacological, and physiological approaches, we show here that anandamide within a very narrow range regulates blastocyst function and implantation by differentially modulating mitogen-activated protein kinase signaling and Ca2+ channel activity via CB1 receptors. Anandamide at a low concentration (7 nM) induces extracellular regulated kinase phosphorylation and nuclear translocation in trophectoderm cells without influencing Ca2+ channels, and renders the blastocyst competent for implantation in the receptive uterus. In contrast, anandamide at a higher concentration (28 nM) inhibits Ca2+ channel activity and blastocyst competency for implantation without influencing mitogen-activated protein kinase signaling. Besides uncovering a potentially important regulatory mechanism for synchronizing blastocyst and uterine competency to implantation, this observation has high clinical relevance, because elevated levels of anandamide induce spontaneous pregnancy loss in women. 相似文献
10.
Foroogh sadat Mansoori Amir Sattari Reza Kheirandish Marziyeh Asli 《Comparative clinical pathology》2014,23(1):125-128
The rabbit fish is an economically valuable teleost species which lives in shallow coastal waters amongst aquatic plants. Two species of rabbit fish, Siganus sutore and Siganus javus, have been identified in the sea south of Iran. In this study, in order to investigate the histology of the outer layer of the S. javus eye, the eyes of 12 healthy specimens of S. javus were extracted and histologic sections were prepared. The sections were studied under a light microscope after staining with hematoxylin and eosin and Masson's trichrome stains. The outer layer was composed of the cornea cranially and the sclera caudally. The sclera contained an episclera zone and two cartilaginous segments with connective tissue correlation. The cornea included dermal components (stratified cuboidal epithelium, Bowman's membrane, and dermal stroma with occasional flattened cells); the scleral part consisted of two main layers (irregular fibers of connective tissue overlaying the second scleral stroma layer). The fibers of the scleral stroma were the only component present at the posterior part of the cornea. These results reveal that the eye of S. javus does not possess Descemet's membrane or endothelium in the cornea. 相似文献