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1.
Alternative splicing: a mechanism for phenotypic rescue of a common inherited defect. 总被引:11,自引:2,他引:9
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![点击此处可从《The Journal of clinical investigation》网站下载免费的PDF全文](/ch/ext_images/free.gif)
H Morisaki T Morisaki L K Newby E W Holmes 《The Journal of clinical investigation》1993,91(5):2275-2280
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QuickVue Influenza Test for Rapid Detection of Influenza A and B Viruses in a Pediatric Population
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Caroline Quach Diane Newby Ghislaine Daoust Earl Rubin Jane McDonald 《Clinical and Vaccine Immunology : CVI》2002,9(4):925-926
The performance of a lateral-flow immunoassay, the QuickVue Influenza Test, for detection of influenza A and B viruses in comparison with that of cell culture was evaluated by using nasopharyngeal aspirates, in viral transport medium, from children with respiratory tract infections. The sensitivity and specificity were 79.2 and 82.6%, respectively. 相似文献
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The synthesis and localization of alternatively spliced fibronectin EIIIB in resting and thrombin-treated megakaryocytes 总被引:1,自引:1,他引:0
There are several species of alternatively spliced fibronectin (FN). One of these, FN EIIIB, is primarily present in embryonic and in proliferating and migrating cells and is believed to be important for cell maturation. We have studied the synthesis, localization, and secretion of this FN isoform in isolated guinea pig megakaryocytes, nonmegakaryocytic bone marrow cells, and platelets. There was 7.5 times more general FN in megakaryocytes than in nonmegakaryocytic cells based on the analysis of equivalent amounts of protein. FN EIIIB was detected by Western blotting in megakaryocytes but not in nonmegakaryocytic cells present in bone marrow. Neither megakaryocytes nor platelets secreted FN EIIIB, while megakaryocytes secreted 25.3% +/- 4.6% general FN and platelets secreted about 61% general FN in response to thrombin. Analysis of immunostained cells by confocal microscopy revealed that FN EIIIB had been redistributed to the surface of megakaryocytes in response to thrombin. Synthesis was studied by metabolic labeling, and megakaryocytes were shown to synthesize FN and FN EIIIB. Thus, megakaryocytes and platelets are among a small number of adult cells and tissues that synthesize and contain FN EIIIB. The expression of FN EIIIB on the megakaryocyte surface may influence migration and maturation. 相似文献
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Altered polyvinylpyrrolidone clearance and immune responsiveness caused by small dietary changes.
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The introduction of 25 mg of a new protein antigen into the diets of mice produced transient increases in PVP clearance which reached a maximum 60 hr after the change of diet and had disappeared by 4 days. At the same time the immune responsiveness to unrelated antigens given parenterally was also raised transiently, reaching a peak 5 days following the change and disappearing by 7 days. In contrast the responsiveness to the fed antigen was diminished. 相似文献