全文获取类型
收费全文 | 750篇 |
免费 | 54篇 |
国内免费 | 14篇 |
专业分类
耳鼻咽喉 | 1篇 |
儿科学 | 35篇 |
妇产科学 | 8篇 |
基础医学 | 86篇 |
口腔科学 | 11篇 |
临床医学 | 52篇 |
内科学 | 183篇 |
皮肤病学 | 21篇 |
神经病学 | 24篇 |
特种医学 | 145篇 |
外科学 | 68篇 |
综合类 | 22篇 |
一般理论 | 1篇 |
预防医学 | 31篇 |
眼科学 | 9篇 |
药学 | 88篇 |
肿瘤学 | 33篇 |
出版年
2022年 | 2篇 |
2021年 | 8篇 |
2020年 | 3篇 |
2019年 | 6篇 |
2018年 | 8篇 |
2017年 | 2篇 |
2016年 | 10篇 |
2015年 | 19篇 |
2014年 | 15篇 |
2013年 | 17篇 |
2012年 | 21篇 |
2011年 | 37篇 |
2010年 | 28篇 |
2009年 | 35篇 |
2008年 | 27篇 |
2007年 | 40篇 |
2006年 | 34篇 |
2005年 | 39篇 |
2004年 | 35篇 |
2003年 | 25篇 |
2002年 | 26篇 |
2001年 | 25篇 |
2000年 | 18篇 |
1999年 | 15篇 |
1998年 | 25篇 |
1997年 | 41篇 |
1996年 | 26篇 |
1995年 | 26篇 |
1994年 | 20篇 |
1993年 | 6篇 |
1992年 | 11篇 |
1991年 | 5篇 |
1990年 | 15篇 |
1989年 | 15篇 |
1988年 | 9篇 |
1987年 | 15篇 |
1986年 | 10篇 |
1985年 | 11篇 |
1984年 | 7篇 |
1983年 | 10篇 |
1982年 | 8篇 |
1981年 | 10篇 |
1980年 | 12篇 |
1979年 | 4篇 |
1978年 | 7篇 |
1977年 | 8篇 |
1976年 | 9篇 |
1975年 | 6篇 |
1974年 | 3篇 |
1971年 | 2篇 |
排序方式: 共有818条查询结果,搜索用时 562 毫秒
1.
L. L. Morselli L. Manetti C. Cosci C. Sardella F. Bogazzi C. Faldini E. Martino M. Gasperi 《Journal of orthopaedics and traumatology》2006,7(4):169-175
Growth hormone (GH) is fundamental for the maintenance of bone mass and metabolism both during childhood and in adulthood.
This effect is due to a complex interaction between circulating GH and IGF-I produced peripherally. In vitro data and experimental
animal models have clarified many of the regulatory mechanisms underlying the characteristic skeletal changes occurring in
acromegaly. This review focuses on the effects of GH excess on bone metabolism and mass in acromegalic patients and, in particular,
on the influence of factors such as hypogonadism, gender, age and therapy on bone metabolism and arthropathy. 相似文献
2.
3.
4.
AG Nettetal 《MedR Medizinrecht》2007,25(11):664-666
Abstrakt 1. Nimmt ein Patient einen ihm von seinem (Zahn-)Arzt einger?umten Exklusiv-Termin nicht wahr, obwohl er auf dessen Eigenschaft
ausdrücklich hingewiesen wurde, so hat er dem (Zahn-)Arzt den Behandlungsausfall abzüglich eines angemessenen Eigenanteils
des (Zahn-)Arztes zu ersetzen.
2. Die Ersatzpflicht tritt auch dann ein, wenn der Patient den Termin nicht in der in dem Behandlungsvertrag vorgesehenen
Frist absagt. Eine hierfür seitens des (Zahn-)Arztes bestimmte Frist von zwei Tagen vor Behandlungsbeginn stellt sich für
den Patienten grunds?tzlich auch nicht als unangemessene Benachteiligung i.S. des § 307 BGB dar.
3. Ein Anspruch des Arztes entf?llt auch bei nur mündlicher Vereinbarung nicht unter dem Gesichtspunkt des § 4 Abs. 5b BMV-Z,
denn diese Vorschrift ist teleologisch dahin zu reduzieren, dass nur zahn?rztliche Honoraransprüche aus erfolgten Behandlungen
schriftlich vereinbart werden müssen. Soweit es jedoch um einen vertraglichen Anspruch wegen einer Leistungsst?rung geht,
vermag das Schriftformerfordernis des § 4 Abs. 5b BMV-Z grunds?tzlich nicht einzugreifen. (Leits?tze des Bearbeiters) 相似文献
5.
Helicobacter pylori cytotoxin: importance of native conformation for induction of neutralizing antibodies. 总被引:22,自引:3,他引:19 下载免费PDF全文
R Manetti P Massari D Burroni M de Bernard A Marchini R Olivieri E Papini C Montecucco R Rappuoli J L Telford 《Infection and immunity》1995,63(11):4476-4480
We have attempted to express the Helicobacter pylori vacuolating cytotoxin in Escherichia coli. Although the 95-kDa VacA polypeptide was expressed abundantly, it completely lacked any biological activity. In addition, this material failed to induce neutralizing antibodies after immunization of rabbits. In contrast, highly purified high-molecular-mass cytotoxin from the supernatant of H. pylori cultures was active in a HeLa cell assay and effectively induced a neutralizing response in rabbits. Neutralizing sera were shown to contain a high proportion of antibodies which recognized conformational epitopes found only on the native toxin. The data indicate that toxin-neutralizing epitopes are conformational and that potential vaccines based on the cytotoxin may benefit from the use of the intact molecule. 相似文献
6.
7.
Early during rat thymus ontogeny, an important proportion of thymocytes
expresses IL-2R and contains IL-2 mRNA. To investigate the role of the
IL-2-IL-2R complex in rat T cell maturation, we supplied either recombinant
rat IL-2 or blocking anti-CD25 mAb to rat fetal thymus organ cultures
(FTOC) under several experimental conditions. The IL-2 treatment initially
stimulated the growth of thymocytes and, as a result, induced T cell
differentiation, but the continuous addition of IL-2 to rat FTOC, as well
as the anti-CD25 administration, resulted in cell number decrease and
inhibition of thymocyte maturation. These results indicate that immature
rat thymocytes bear functional high- affinity IL-2R and that IL-2 promotes
T cell differentiation as a consequence of its capacity to stimulate cell
proliferation. Modifications in TCR alpha beta repertoire and increased
numbers of NKR- P1+ cells, largely NK cells, were also observed in
IL-2-treated FTOC. Furthermore, IL-2-responsiveness of different thymocyte
subsets changed throughout thymic ontogeny. Immature CD4-CD8-cells
responded to IL-2 in two stages, early in thymus development and around
birth, in correlation with the maturation of two distinct waves of thymic
cell progenitors. Mature CD8+ thymocytes maximally responded to IL-2 around
birth, supporting a role for IL-2 in the increased proliferation of mature
thymocytes observed in vivo in the perinatal period. Taken together, these
findings support a role for IL-2 in rat T cell development.
相似文献
8.
Ghosh D; Stewart DR; Nayak NR; Lasley BL; Overstreet JW; Hendrickx AG; Sengupta J 《Human reproduction (Oxford, England)》1997,12(5):914-920
The present study was undertaken to assess the temporal association between
the profiles of serum concentrations of oestradiol-17beta, progesterone,
chorionic gonadotrophin (CG) and relaxin in pregnancies established
naturally, and after embryo transfer, as well as in failed pregnancies in
rhesus monkeys. In naturally mated cycles (group 1) a conception rate of
75% was obtained. In group 1, the mean day of CG detection in serum was
11.5 +/- 1.9 day post-ovulation, and for relaxin, 9.0 +/- 2.5 day
post-ovulation. In group 2, embryo transfer to synchronous, non-mated
surrogate recipients was performed; seven embryo transfer cycles yielded
three pregnancies which were allowed to continue to term and normal infants
were delivered. In embryo transfer cycles the mean day of CG detection was
14.8 +/- 1.8 day post- ovulation, and for relaxin, 11.4 +/- 2.6 day
post-ovulation. A delay of about 3 days was observed in the appearance in
circulation of CG (P < 0.05) and also of relaxin (P < 0.05) between
natural mated and embryo transfer conception cycles. Significant
differences (P < 0.05 for progesterone and P < 0.03 for oestradiol)
were obtained for the areas under the curves for progesterone and
oestradiol between days 12 and 16 in conception cycles compared with failed
pregnancies. These data provide the first observation of the normal
hormonal signals associated with maternal recognition of transferred
embryos during the peri- implantation period, and suggest that the use of
such an experimental primate embryo transfer model may help to elucidate
components of maternal and embryonic signal-response mechanisms during
embryo implantation.
相似文献
9.
A rapid microagglutination test for the diagnosis of Legionella pneumophila (serogroup 1) infection 总被引:4,自引:3,他引:4 下载免费PDF全文
A rapid microagglutination test has been developed which can be performed in 30 minutes. Ninety-seven percent of 96 patients diagnosed as having Legionella pneumophila (serogroup 1) infection by indirect immunofluorescence were also detected by the rapid microagglutination test. 相似文献
10.
Characterization of genetically inactivated pertussis toxin mutants: candidates for a new vaccine against whooping cough. 总被引:4,自引:16,他引:4 下载免费PDF全文
L Nencioni M Pizza M Bugnoli T De Magistris A Di Tommaso F Giovannoni R Manetti I Marsili G Matteucci D Nucci et al. 《Infection and immunity》1990,58(5):1308-1315
the introduction of two amino acid substitutions within the enzymatically active subunit S1 of pertussis toxin (PT) abolishes its ADP-ribosyltransferase activity and toxicity on CHO cells (Pizza et al., Science 246:497-500, 1989). These genetically inactivated molecules are also devoid of other in vivo adverse reactions typical of PT, such as induction of leukocytosis, potentiation of anaphylaxis, stimulation of insulin secretion, and histamine sensitivity. However, the mutant PT molecules are indistinguishable from wild-type PT in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and maintain all the physical and chemical properties of PT, including affinity for toxin-neutralizing poly- and monoclonal antibodies. Either alone or stabilized with formaldehyde, PT mutants are able to induce high levels of neutralizing antibodies and to protect mice in a dose-dependent fashion against intracerebral challenge with virulent B. pertussis. These results clearly show that these genetically inactivated PT molecules are nontoxic but still immunogenic and justify their development as a component of a new, safer acellular vaccine against whooping cough. 相似文献