Citrus hallabong [(Citrus unshiu × C. sinensis) × C. reticulata)] exerts potent anti-inflammatory properties in murine splenocytes and TPA-induced murine ear oedema model

Context: Hallabong [(Citrus unshiu?×?C. sinensis) X C. reticulata)] (Rutaceae) is a hybrid citrus cultivated in temperate regions of South Korea. Its fruit is well-known for pharmacological properties.

Objective: This study examined the anti-inflammatory effect of 80% ethanol extract of Hallabong (HE) on concanavalin A (Con A)-stimulated splenocytes and mouse oedema model induced by 12-O-tetradecanoylphorbal acetate (TPA).

Materials and methods: Murine splenocytes treated with HE were stimulated with Con A (10?μg/mL, for 24?h) were evaluated for T-cell population and production of inflammatory cytokines IL-2, IL-4 and IFN-γ. Anti-inflammatory effect of topically applied HE (100?μg/20?μL) on TPA (4?μg/20?μL/ear)-induced ear oedema was investigated in mouse model.

Results: HE-treated Con A-stimulated murine splenocytes showed a marked decrease in CD44/CD62L⁺ memory T-cell population, an important marker for anti-inflammatory activity, and a significant inhibition in the production of IL-2 and IFN-γ. HE treatment had reduced the mouse skin oedema (47%) and myeloperoxidase (MPO) activity significantly (40%) in TPA-challenged tissues. More importantly, immunohistochemical localization revealed the suppressed (p?<?0.05) expression of inducible nitric oxide (iNOS), cyclooxygenase-2 (COX2). HE decreased the infiltration of CD3⁺ T cells and F4/80⁺ macrophages to the site of inflammation and a topical application of HE significantly suppressed the expression of TNF-α (20.2%).

Discussion and conclusion: A topical application of HE can exert a potential anti-inflammatory effect and HE can be explored further as a putative alternative therapeutic agent for inflammatory oedema.     

Sasa quelpaertensis leaves ameliorate alcohol-induced liver injury by attenuating oxidative stress in HepG2 cells and mice

Oxidative stress plays a crucial role in the progression of alcoholic liver diseases and substances of antioxidant property are of special interest for therapeutic purposes. We investigated the hepatoprotective effect of leaf extracts of Sasa quelpaertensis, an edible bamboo mainly cultivated in Jeju Island, South Korea. We examined the cytotoxicity of different extracts (distilled water, 20–80% EtOH) of S. quelpaertensis on HepG2 cells and their hepatoprotective effect on HepG2 cells stimulated by ethanol (800?mM, 24?h). Furthermore, we measured reactive oxygen species (ROS) production, ethanol toxicity induced cell death, and the activity of antioxidant enzymes. In in vivo experiments, liver damage was induced by oral administration of 5?g/kg ethanol with or without potent ethanol extract of S. quelpaertensis (10 or 100?mg/kg) 12?h interval for a total of 3 doses. Only 80% ethanol extract of S. quelpaertensis (SQEE80) exhibited cytoprotective effect on HepG2 cells against alcohol-induced toxicity. SQEE80 treatment (250, 500?μg/mL) in ethanol exposed HepG2 cells showed significant attenuation of ROS production and ethanol toxicity induced cell death. Furthermore, SQEE80 markedly increased the activity of antioxidant enzyme glutathione peroxidase 1 in ethanol exposed HepG2 cells compared to ethanol stimulated cells. In in vivo experiments, SQEE80 treatment evidently suppressed the alcohol-induced histopathological changes in liver, serum ethanol content, and expression of cytochrome P450 2E1. Furthermore, SQEE80 significantly reversed the reduction of glutathione level in the ethanol challenged liver. Taken together, we suggest the possibility of developing SQEE80 as a natural hepatoprotective substance in attenuating alcohol-induced oxidative stress.     

Recent breakthroughs in nanostructured antiviral coating and filtration materials: a brief review

COVID-19 persists as the most challenging pandemic of the 21^st century with a high rate of transmission. The main pathway of SARS-CoV-2 transmission is aerosol-mediated infection transfer through virus-laden droplets that are expelled by infected people, whereas indirect transmission occurs when contact is made with a contaminated surface. This mini review delivers an overview of the current state of knowledge, research directions, and applications by examining the most recent developments in antiviral surface coatings and filters and analyzing their efficiencies. Reusable masks and other personal protective devices with antiviral properties and self-decontamination could be valuable tools in the fight against viral spread. Moreover, antiviral surface coatings that repel pathogens by preventing adhesion or neutralize pathogens with self-sanitizing ability are assumed to be the most desirable for terminating indirect transmission of viruses. Although many nanomaterials have shown high antiviral capacities, additional research is unquestionably required to develop next-generation antiviral agents with unique characteristics to face future viral outbreaks.

Types of antiviral nanofilters and coatings and their applications.