全文获取类型
收费全文 | 836篇 |
免费 | 54篇 |
国内免费 | 1篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 45篇 |
妇产科学 | 9篇 |
基础医学 | 161篇 |
口腔科学 | 16篇 |
临床医学 | 84篇 |
内科学 | 159篇 |
皮肤病学 | 32篇 |
神经病学 | 76篇 |
特种医学 | 16篇 |
外科学 | 55篇 |
综合类 | 9篇 |
一般理论 | 3篇 |
预防医学 | 65篇 |
眼科学 | 35篇 |
药学 | 80篇 |
中国医学 | 1篇 |
肿瘤学 | 39篇 |
出版年
2024年 | 2篇 |
2023年 | 10篇 |
2022年 | 24篇 |
2021年 | 45篇 |
2020年 | 20篇 |
2019年 | 26篇 |
2018年 | 31篇 |
2017年 | 9篇 |
2016年 | 20篇 |
2015年 | 32篇 |
2014年 | 29篇 |
2013年 | 47篇 |
2012年 | 77篇 |
2011年 | 48篇 |
2010年 | 35篇 |
2009年 | 22篇 |
2008年 | 52篇 |
2007年 | 67篇 |
2006年 | 51篇 |
2005年 | 36篇 |
2004年 | 37篇 |
2003年 | 36篇 |
2002年 | 37篇 |
2001年 | 8篇 |
2000年 | 3篇 |
1999年 | 4篇 |
1998年 | 8篇 |
1997年 | 3篇 |
1996年 | 6篇 |
1995年 | 2篇 |
1994年 | 4篇 |
1993年 | 4篇 |
1989年 | 3篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1984年 | 4篇 |
1978年 | 2篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1935年 | 1篇 |
1933年 | 5篇 |
1931年 | 2篇 |
1929年 | 1篇 |
1928年 | 1篇 |
1927年 | 1篇 |
1925年 | 3篇 |
1922年 | 1篇 |
1912年 | 2篇 |
1911年 | 1篇 |
排序方式: 共有891条查询结果,搜索用时 15 毫秒
1.
Johan Siebert Klara M. Posfay-Barbe† Walid Habre‡ Claire-Anne Siegrist 《Paediatric anaesthesia》2007,17(12):1218-1220
2.
Carmem Lúcia Pessoa-Silva Sasi Dharan Stéphane Hugonnet Sylvie Touveneau Klara Posfay-Barbe Riccardo Pfister Didier Pittet 《Infection control and hospital epidemiology》2004,25(3):192-197
OBJECTIVE: To evaluate the dynamics of bacterial contamination of healthcare workers' (HCWs) hands during neonatal care. SETTING: The 20-bed neonatal unit of a large acute care teaching hospital in Geneva, Switzerland. METHODS: Structured observation sessions were conducted. A sequence of care began when the HCW performed hand hygiene and ended when the activity changed or hand hygiene was performed again. Alcohol-based handrub was the standard procedure for hand hygiene. An imprint of the five fingertips of the dominant hand was obtained before and after hand hygiene and at the end of a sequence of care. Regression methods were used to model the final bacterial count according to the type and duration of care and the use of gloves. RESULTS: One hundred forty-nine sequences of care were observed. Commensal skin flora comprised 72.4% of all culture-positive specimens (n = 360). Other microorganisms identified were Enterobacteriaceae (n = 55, 13.8%); Staphylococcus aureus (n = 10, 2.5%); and fungi (n = 7, 1.8%). Skin contact, respiratory care, and diaper change were independently associated with an increased bacterial count; the use of gloves did not fully protect HCWs' hands from bacterial contamination. CONCLUSIONS: These data confirm that hands become progressively contaminated with commensal flora and potential pathogens during neonatal care, and identify activities at higher risk for hand contamination. They also reinforce the need for hand hygiene after a sequence of care, before starting a different task, and after glove removal. 相似文献
3.
Angelo Pan Placido Mondello Klara Posfay-Barbe Patrizia Catenazzi Annise Grandi Silvia Lorenzotti Andrea Patroni Nadia Poli Laura Soavi Giuseppe Carnevale 《Infection control and hospital epidemiology》2007,28(9):1099-1102
In an Italian hospital, we observed that hand hygiene was performed in 638 (19.6%) of 3,253 opportunities, whereas gloves were worn in 538 (44.2%) 1,218 of opportunities. We observed an inverse correlation between the intensity of care and the rate of hand hygiene compliance (R2=0.057; P<.001), but no such association was observed for the rate of glove use compliance (R2=0.014; P=.078). Rates of compliance with hand hygiene and glove use recommendations follow different behavioral patterns. 相似文献
4.
5.
Meiosis was examined in pollen mother cells of F1 hybrids made from crosses between wheat (Triticum aestivum) and lymegrass (Leymus arenarius and L. mollis). Fluorescence genomic in situ hybridization detected pairing between wheat and lymegrass chromosomes during prophase I and metaphase I. Such pairing, when resulting in bivalent formation, was likely to yield correct disjunction, and hence intergenomic recombination could be incorporated into the gametes. Bivalents in these hybrids, however, were more frequently formed between chromosomes of the same parental origin. Univalents were common, whereas multivalents were not clearly detected. Meiotic behaviour in some cells was not totally aberrant, and this may have accounted for the presence of normal pollen. The results are discussed in relation to intergenomic pairing, meiotic behaviour in wide-hybrids and genome relationships, including the Leymus genome origin. 相似文献
6.
Wolenski M Cramer SO Ehrlich S Steeg C Grossschupff G Tenner-Racz K Racz P Fleischer B von Bonin A 《Medical microbiology and immunology》2003,192(4):189-192
CD83 is used as a marker for mature dendritic cells (DC) in man. We have developed a new monoclonal antibody (mAb), Michel-17, that specifically recognizes mouse CD83. We show that murine CD83 is expressed mainly on mature DC and on activated T cells. Histological analysis of serial spleen sections revealed a CD83 expression pattern resembling that of MIDC-8, a known murine DC marker molecule. In contrast to other costimulatory receptors, cross-linking of CD83 with the mAb Michel-17 on DC or T cells does not induce any activation signals. Our data describe for the first time the expression pattern of murine CD83, which is comparable to that of human CD83.The unique mAb Michel-17 will help to elucidate the biological functions of the CD83 molecule in more detail. 相似文献
7.
Frei K Szuhai K Lucas T Weipoltshammer K Schöfer C Ramsebner R Baumgartner WD Raap AK Bittner R Wachtler FJ Kirschhofer K 《European journal of human genetics : EJHG》2002,10(7):427-432
Mutations in the connexin 26 (Cx26) gene (GJB2) are associated with autosomal nonsyndromic sensorineural hearing loss. This study describes mutations in the Cx26 gene in cases of familial and sporadic hearing loss (HL) by gene sequencing and identifies the allelic frequency of the most common mutation leading to HL (35delG) in the population of eastern Austria. For this purpose we have developed and applied a molecular beacon based real-time mutation detection assay. Mutation frequencies in the Cx26 gene of individuals from affected families (14 out of 46) and sporadic cases (11 out of 40) were 30.4% and 27.5%, respectively. In addition to known disease related alterations, a novel mutation 262 G-->T (A88S) was also identified. 35delG accounted for almost 77% of all Cx26 mutations detected and displayed an allelic frequency in the normal hearing population of 1.7% (2 out of 120). The high prevalence of the 35delG mutation in eastern Austria would therefore allow screening of individuals and family members with Cx26 dependent deafness by a highly specific and semi-automated method. 相似文献
8.
Daniel Hork Franti eksvec Jaroslav Klal Arnold A. Adamyan Yurii D. Volynskii Olga S. Voronkova Leonid S. Kokov Klara Z. Gumargalieva 《Biomaterials》1986,7(6):467-470
In this study we report the results of clinical experiments, obtained with spherical particles made from poly(2-hydroxyethyl methacrylate) used in the embolization of arteriovenous anastomoses, in the suppression of pulmonary haemorrhage and haemoptysis and in the occlusion of some other arteries. So far we have used these particles in the treatment of 187 patients. It must be stressed that the advantage of spherical particles consists in the simplicity of their introduction into the blood vessel through a catheter, while in the blood vessel itself the particle swells in blood still more, when compared with the particle size in saline. This results in an immediate and permanent haemostatic effect. No revascularization occurs. 相似文献
9.
This study examined the interaction between working memory and language comprehension in children with specific language impairment (SLI), focusing on the function of the central executive component and its interaction with the phonological loop (A. D. Baddeley, 1986) in complex working memory tasks. Thirteen children with SLI and 13 age-matched (age range = 7;0 [years;months] to 10;0) children with typical language development participated. The tasks combined traditional nonword repetition tests and sentence comprehension by using sentences that differed in length and syntactic complexity. The children with SLI exhibited larger processing and attentional capacity limitations than their age-matched peers. Increased word length and syntactic complexity resulted in a large performance decrease in nonword repetition in both groups. There were some variations in the error pattern, which may indicate qualitative differences between the 2 groups. The performance of the children with SLI in nonword repetition, across the different tasks, indicated a limitation in simultaneous processing rather than difficulty in encoding and analyzing the phonological structure of the nonwords. Furthermore, syntactic complexity had a greater effect on performance accuracy than did sentence length. 相似文献
10.
Kathrin Mühlemann Christine Franzini Christoph Aebi Christoph Berger David Nadal Jody St?helin Hanspeter Gnehm Klara Posfay-Barbe Alain Gervaix Hugo Sax Ulrich Heininger Jan Bonhoeffer Gerhard Eich Christian Kind Christiane Petignat Pietro Scalfaro 《Infection control and hospital epidemiology》2004,25(9):765-771
OBJECTIVE: To acquire data on pediatric nosocomial infections (NIs), which are associated with substantial morbidity and mortality and for which data are scarce. DESIGN: Prevalence survey and evaluation of a new comorbidity index. SETTING: Seven Swiss pediatric hospitals. PATIENTS: Those hospitalized for at least 24 hours in a medical, surgical, intensive care, or intermediate care ward. RESULTS: Thirty-five NIs were observed among 520 patients (6.7%; range per hospital, 1.4% to 11.8%). Bacteremia was most frequent (2.5 per 100 patients), followed by urinary tract infection (1.3 per 100 patients) and surgical-site infection (1.1 per 100 patients; 3.2 per 100 patients undergoing surgery). The median duration until the onset of infection was 19 days. Independent risk factors for NI were age between 1 and 12 months, a comorbidity score of 2 or greater, and a urinary catheter. Among surgical patients, an American Society of Anesthesiologists (ASA) score of 2 or greater was associated with any type of NI (P = .03). Enterobacteriaceae were the most frequent cause of NI, followed by coagulase-negative staphylococci; viruses were rarely the cause. CONCLUSIONS: This national prevalence survey yielded valuable information about the rate and risk factors of pediatric NI. A new comorbidity score showed promising performance. ASA score may be a predictor of NI. The season in which a prevalence survey is conducted must be considered, as this determines whether seasonal viral infections are observed. Periodic prevalence surveys are a simple and cost-effective method for assessing NI and comparing rates among pediatric hospitals. 相似文献