全文获取类型
收费全文 | 2330篇 |
免费 | 79篇 |
国内免费 | 9篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 26篇 |
妇产科学 | 45篇 |
基础医学 | 324篇 |
口腔科学 | 36篇 |
临床医学 | 104篇 |
内科学 | 532篇 |
皮肤病学 | 10篇 |
神经病学 | 98篇 |
特种医学 | 86篇 |
外科学 | 434篇 |
综合类 | 7篇 |
预防医学 | 156篇 |
眼科学 | 7篇 |
药学 | 81篇 |
中国医学 | 1篇 |
肿瘤学 | 458篇 |
出版年
2023年 | 12篇 |
2022年 | 33篇 |
2021年 | 63篇 |
2020年 | 24篇 |
2019年 | 43篇 |
2018年 | 47篇 |
2017年 | 41篇 |
2016年 | 45篇 |
2015年 | 39篇 |
2014年 | 58篇 |
2013年 | 74篇 |
2012年 | 147篇 |
2011年 | 159篇 |
2010年 | 90篇 |
2009年 | 78篇 |
2008年 | 132篇 |
2007年 | 152篇 |
2006年 | 161篇 |
2005年 | 162篇 |
2004年 | 186篇 |
2003年 | 152篇 |
2002年 | 168篇 |
2001年 | 28篇 |
2000年 | 30篇 |
1999年 | 28篇 |
1998年 | 42篇 |
1997年 | 28篇 |
1996年 | 23篇 |
1995年 | 17篇 |
1994年 | 17篇 |
1993年 | 16篇 |
1992年 | 14篇 |
1991年 | 13篇 |
1990年 | 12篇 |
1989年 | 9篇 |
1988年 | 11篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1984年 | 14篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 2篇 |
1980年 | 4篇 |
1978年 | 2篇 |
1977年 | 3篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有2418条查询结果,搜索用时 15 毫秒
1.
Shinichi Toyooka Hideaki Mori Katsuyuki Kiura Hiroshi Date 《European journal of cardio-thoracic surgery》2008,33(2):315-316
We present a case of a 58-year-old man with diagnosis of lung adenocarcinoma invading the left atrium. He was treated with induction chemoradiotherapy for T4N1M0 disease, showing objective response. Then, a left upper lobectomy with a partial resection of the left atrium was performed without cardiopulmonary bypass. No residual tumor cells existed in the resected specimens, showing pathological complete response. Our case suggests that induction chemoradiotherapy prior to surgery can be an appropriate strategy among carefully selected patients with non-small cell lung cancer invading the left atrium. 相似文献
2.
Eiji Fukuyama Susumu Omura Kiyohide Fujita Kunimichi Soma Katsuyuki Torikai 《The Cleft palate-craniofacial journal》2006,43(6):673-677
OBJECTIVE: This article reports a case of bilateral cleft lip and alveolus (BCLA) for which excessive rapid palatal expansion with a Latham appliance was performed for preoperative alignment of the protruded premaxilla. Postoperative changes of maxillary width were investigated with serial plaster casts. PATIENT AND RESULTS: A 3-month-old girl presented with complete BCLA in which the premaxilla was markedly protruded. Preoperative alignment of the protruded premaxilla with a Latham appliance was planned to facilitate primary lip repair. The appliance was placed when the patient was 4.5 months old. The necessary palatal expansion was estimated to be 7.0 mm in order to move the premaxilla backward into the ideal position. After palatal expansion and posterior repositioning of the protruded premaxilla, the primary operation, including cheiloplasty and gingivoperiosteoplasty, was performed when the patient was 7 months old. Excessive maxillary expansion might be a cause of transverse maxillomandibular discrepancy. Measurement with serial plaster casts demonstrated that maxillary widths increased from 42.3 mm pretreatment to 49.0 mm after orthopedic treatment but relapsed markedly to 43.5 mm at 3 months after the primary operation. Therefore, the net change of maxillary widths was only 1.2 mm. After alignment of the protruded premaxilla, tension-free soft tissue repairs were performed, and a harmonious alveolar arch was obtained without change in maxillary width. CONCLUSION: These results indicate that this method is useful for preoperative management of BCLA with protruded premaxilla. 相似文献
3.
Yoichi Kikuchi Taku Sakurada Masato Suzuki Tomoyasu Hirano Katsuyuki Kusajima 《The Japanese Journal of Thoracic and Cardiovascular Surgery》2004,52(8):375-378
Off-pump coronary artery bypass grafting has become an attractive surgical alternative for myocar-dial revascularization because
of the advantage of myocardial protection and other benefits of patients. However, it is still regarded as a controversial
treatment for the coronary artery disease accompanied by atrial fibrillation (AF). A significant number of patients in need
of coronary revascularization have chronic AF. Although the Cox-Maze III procedure is the gold standard for the surgical treatment
of AF, few of these patients undergo AF operations at the time of their coronary bypass grafting. We report herein a case
of the pulmonary vein isolation to eliminate the AF by means of epicardial radiofrequency ablation combined with 2 vessels
coronary artery bypass grafting on the beating heart with the aid of cardiopulmonary bypass. 相似文献
4.
A case of small cell carcinoma of the prostate without a primary lesion in the lung was reported. The cancer was diagnosed after the patient complained of lumbago caused by bone metastasis. The tumor was 5.9 times 5.0 times 4.6cm. The patient was treated with 4 courses of chemotherapy using cisplatin and etoposide. The tumor diminished to 4.0 times 4.0 times 3.5 cm after completion of the 4 courses of treatment. Prostatic antigen levels were less than 1.0ng/mL during the therapy. Neuron-specific enolase levels were 35.9ng/mL at the beginning of therapy, and decreased to 7.4 ng/mL after completion of 4 courses of treatment. The patient died 3 months after the completion of treatment. This regimen had some value for inhibiting the growth of small cell carcinoma. 相似文献
5.
Park JW Taube C Swasey C Kodama T Joetham A Balhorn A Takeda K Miyahara N Allen CB Dakhama A Kim SH Dinarello CA Gelfand EW 《American journal of respiratory cell and molecular biology》2004,30(6):830-836
The role of an interleukin (IL)-1 receptor antagonist (IL-1Ra) on the development of airway hyperresponsiveness (AHR) and airway inflammation following acute O(3) exposure in mice was investigated. Exposure of C57/BL6 mice to O(3) at a concentration of 2.0 ppm or filtered air for 3 h resulted in increases in airway responsiveness to inhaled methacholine (MCh) 8 and 16 h after the exposure, and an increase in neutrophils in the bronchoalveolar lavage (BAL) fluid. IL-1beta expression, assessed by gene microarray, was increased 2-fold 4 h after O(3) exposure, and returned to baseline levels by 24 h. Levels of IL-1beta in lung homogenates were also increased 8 h after O(3) exposure. Administration of (human) IL-1Ra before and after O(3) exposure prevented development of AHR and decreased BAL fluid neutrophilia. Increases in chemokine levels in lung homogenates, tumor necrosis factor-alpha, MIP-2, and keratinocyte chemoattractant following O(3) exposure were prevented by IL-1Ra. Inhalation of dexamethasone, an inhibitor of IL-1 production, blocked the development of AHR, BAL fluid neutrophilia, and decreased levels of IL-1 following O(3) exposure. In summary, acute exposure to O(3) induces AHR, neutrophilic inflammation, epithelial damage, and IL-1. An IL-1Ra effectively prevents the development of altered airway function, inflammation, and structural damage. 相似文献
6.
Fujita K Shimazaki N Ohta Y Kubota T Ibe S Toji S Tamai K Fujisaki S Hayano T Koiwai O 《Genes to cells : devoted to molecular & cellular mechanisms》2003,8(6):559-571
BACKGROUND: Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase that enhances the Ig and TcR gene diversity in the N region at the junctions of variable (V), diversity (D) and joining (J) segments in B- and T-cells. TdT synthesizes the N region in concert with many proteins including DNA-PKcs, Ku70 and Ku86. To elucidate the molecular mechanism of the N region synthesis, we first attempted to isolate the genes with products that directly interact with TdT. RESULTS: Using a yeast two-hybrid system, we isolated a cDNA clone encoding a novel nuclear protein that interacts with TdT. This protein was designated as TdT interacting factor 2 (TdIF2). The confined region of the C-terminal in TdIF2 is involved in specific interaction with the entire C-terminal in TdT. TdIF2 contains an acidic region comprised of 42 residues. TdIF2 was shown to bind specifically to a core histone by pull down assay using specific antibodies against TdIF2. When a TdT/TdIF2 complex was applied on to a DNA-cellulose column, only TdT bound to the column while TdIF2 passed through. TdIF2 reduces the TdT activity to 46% of its maximum value in vitro assay system using activated DNA as primer. CONCLUSIONS: TdIF2 binds directly to TdT and core histone. Furthermore, TdT, TdIF2 and core histone form a ternary complex. TdIF2 liberates H2A/H2B from a core histone in correlation with PCNA. The enzymatic consequence of the TdIF2/TdT complex is the reduction of TdT activity in vitro. TdIF2 would function as a chromatin remodeling protein at the N region synthesis. 相似文献
7.
Yoshimura R Matsuyama M Hase T Tsuchida K Kuratsukuri K Kawahito Y Sano H Segawa Y Nakatani T 《International journal of molecular medicine》2003,12(6):861-865
Peroxisome proliferator activator-receptor (PPAR)-gamma ligand induces growth arrest of cancer cells through apoptosis. In this study, we examined the effects of PPAR-gamma inhibitors on cell proliferation in renal cell carcinoma (RCC), bladder tumor (BT), and prostatic carcinoma (PC) cell lines. We investigated the inhibitory effect of PPAR-gamma ligands, troglitazone and 15-deoxy-Delta12,14-prostaglandin J2 (15dPGJ2) on RCC, BT and PC-derived cell lines using MTT assay and Hoechst staining. PPAR-gamma ligands (troglitazone and 15dPGJ2) induced the reduction of cell viability with the half-maximal concentration of growth inhibition of RCC, BT, and PC cell lines. Furthermore, counting cells at days 1, 2 and 3, clearly showed marked inhibition of cell proliferation using troglitazone and 15dPGJ2. All PPAR-gamma inhibitors stopped the growth of all RCC, BT and PC cells. Cells treated with PPAR-gamma inhibitors showed chromatin condensation, cellular shrinkage, small membrane-bound bodies (apoptotic bodies), and cytoplasmic condensation. These cellular changes were typically redundant characteristics of apoptosis. PPAR-gamma ligands may mediate potent antiproliferative effects against RCC, BT and PC cells through differentiation. Thus, PPAR-gamma may become a new target in treatment of urological tumors. 相似文献
8.
9.
Kawada K Yonei T Ueoka H Kiura K Tabata M Takigawa N Harada M Tanimoto M 《Acta medica Okayama》2002,56(3):129-134
When the development of chemotherapeutic agents reaches the clinical trial stage, it is necessary to perform drug sensitivity tests quickly in order to select the most promising agents for the treatment of cancer. In order to assess the possibility of using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay as a substitute for the human tumor clonogenic assay (HTCA), we evaluated the correlation between the results obtained by these 2 assays in 5 human lung cancer cell lines. The correlation coefficient between the results of the HTCA and the MTT assay was 0.673, indicating a relatively good correlation. The correlation was most prominent in platinum analogues (r = 0.939) and good in anthracyclines/anthracenedione (r = 0.611). However, no significant correlation was observed in vinca alkaloids, etoposide, irinotecan, SN-38 (an active metabolite of irinotecan), and rhizoxin. The results of the MTT assay showed a high degree of correlation with those of the HTCA in predicting the sensitivity of cancer cell lines to platinum analogues, and anthracyclines/anthracenedione. These results suggest that the MTT assay may be more convenient and quickly performed than the HTCA and can replace HTCA in evaluating the effects of anticancer agents, especially the platinum analogues and anthracyclines/anthracenedione. 相似文献
10.
Nakamichi I Takakuwa T Tanio Y Iuchi K Aozasa K 《Virchows Archiv : an international journal of pathology》2005,447(5):888-891
Pyothorax-associated lymphoma (PAL) is a B-cell lymphoma which develops in the pleural cavity of patients with an over-20-year
history of pyothorax. Aberrant expression of surface antigens is occassional in PAL, although genotype is not fully investigated.
We report here a PAL with dual genotype, i.e., simultaneous immunoglobin (Ig) and T-cell receptor (TcR) gene rearrangement.
An 82-year-old woman with pain on the left side of the chest was admitted. She had been suffering from pyothorax after artificial
pneumothorax for treatment of tuberculosis of the pulmonary when she was 18 years old. The mass that was confined to the left
pleural cavity affected by pyothorax was biopsied and histologically diagnosed as diffuse large cell lymphoma. The tumor cells
were positive for CD20, CD16, and TIA-1 but negative for CD79a, CD45RO, CD43, CD3, and CD56. Surface antigen expression was
further investigated in cultured cells, showing that the cultured cells did not express representative B-cell markers, except
for CD20, as well as T-cell markers, but were positive for CD16, CD30, and CD103. Southern blotting revealed the monoclonally
rearranged bands of both Ig heavy chain and TcR gene. The patients died of tumors 14 months after admission. Aberrant genotype
and immunophenotype of PAL cells is discussed in reviewing the pertinent literature. 相似文献