Effect of emissions from the Household Glassware Factory (HGF) "Irena" in Inowroc?aw on the levels of fluorine and lead in selected vegetables and fruit harvested in 1988]

Fluorine and lead were determined by spectroscopic methods in some vegetables harvested in 1988 in two groups of Employees' Gardens in Inowroc?aw ("Transportowiec" and "Irena", distant from the Household Glassworks "Irena" by ca. 500 and 2600 m, respectively). Employees' Gardens "Irena" were divided into 2 belts, in consideration of a vicinal traffic artery. It was found that 42% of the plants harvested in Employees' Gardens "Transportowiec" exhibited excessive contents of fluorine, and 91%--of lead; fluorine contamination of vegetables was, however, slight, except for parsley leaves containing 56.0 mg F per 1 kg dry weight. In the case of Employees' Gardens "Irena", in the I belt (30-40 m from the traffic artery) the level of fluorine was excessive in 8.3% of plants, and that of lead--in 63.6% of plants; in the II belt (80-100 m from the traffic artery) the respective values were 17% for fluorine and 66.6% for lead.     

Serum protein profiling to identify high-risk neuroblastoma: preclinical relevance of blood-based biomarkers

INTRODUCTION: Development of early detection assays for advanced stage neuroblastoma (NB) remains elusive. We have previously shown that serum protein profiling technologies can differentiate healthy from NB children. As various sources of patient related bias exist in serum proteins, we hypothesized a well controlled animal model may provide a better method to identify tumor blood-based markers during NB progression. METHODS: Tumors were induced in the left kidneys of nude mice by the injection of cultured human NB cells (10(6)). Sera were collected from control and tumor-bearing mice at 2, 4, and 6 wk. Albumin-depleted sera were subjected to comparative proteomic profiling using 2D gel electrophoresis. Paired samples at each time point were analyzed and differentially expressed serum proteins were identified by mass spectrometry. Additionally, sera proteomic analysis from children with Stage IV NB and healthy controls were performed. RESULTS: Overexpression of five mouse serum proteins [alpha(1)-acid glycoprotein, alpha(1)-antitrypsin, alpha(2)-macroglobulin, serum amyloid P-component, and serum amyloid A) were found only in NB-bearing mice. Changes in protein abundance were found to increase 2.5-fold (P < or = 0.05) between 2-, 4-, and 6-wk old mice. Underexpression of immunoglobulin kappa chain constant region was observed in the sera of tumor bearing mice compared with controls (2.5-fold, P < or = 0.05). Among NB patients, alpha(1)-acid glycoprotein, apolipoprotein A-IV, haptoglobin, and serum amyloid A were found to be up-regulated. CONCLUSIONS: We identified distinct acute phase proteins that show up-regulation in both an animal tumor model and high-risk NB patients. As these serum proteins have been recognized as markers of tumor progression and prognosis in human malignancies, the validation of these polypeptides may enable serum proteomic profiling to become a valuable tool for identifying high-risk NB.     

Unanticipated Cesarean Birth from the Father's Perspective

ABSTRACT: Despite the paucity of research on men's experiences of cesarean birth, fathers' attendance at cesareans has become well-established in some areas of the U.S. In this study, interviews were conducted with 46 fathers whose wives had an uncomplicated pregnancy culminating in an unanticipated cesarean birth with a healthy neonate and no major complications for mother and child. Interviews were tape recorded, transcribed and analyzed. Of these 46 fathers, 52 per cent attended the cesarean, and 48 per cent did not, primarily because hospital policy prohibited it. Fathers' predominant emotional reaction to the decision for cesarean was relief (52%); 27 per cent described their reactions as acceptance, 10 per cent expressed moderate disappointment, and 11 per cent were strongly disappointed or angry. Most negative reactions centered not on the cesarean itself, but on policies which excluded fathers from attendance arbitrarily, and on staff behaviors which reflected disregard for the fathers'need to feel included in the birth, whether they were permitted to attend the delivery or not. Seventy per cent of these fathers expressed some displeasure at physician or nurse behaviors, expressing disappointment and resentment about being excluded from discussions leading to the decision for the cesarean, having little previous contact with the obstetrician, the nursing staff failing to provide the father with adequate information and support during and immediately after the cesarean, and being denied access to the wife and infant after the cesarean for apparently arbitrary reasons.     

Study of fluorine and lead levels in selected vegetables and fruit grown within the range of emission of these elements by the household glass plant "Irena" in Inowroc?aw

The content of fluorine and lead was determined by spectroscopic methods in the vegetables, fruit and flowers gathered in 1987 from the "Transportowiec" Garden Plots situated in the range of emission of these elements by the plant. Fluorine was isolated from plant materials by microdiffusion and determined spectrophotometrically as a triple complex: alizarine complexon + lanthanum + fluorideion. Lead was determined by atomic absorption spectrophotometry (ASA). Vegetables and fruit were raised in the range of 500 m from the emission source of the "Irena" plant. In only several vegetables the content of fluorine calculated for dry mass exceeded the Polish permissible level. On the other hand, the lead content in vegetables exceeded in 18.5% the Polish normal value and the suggested normal value of the Council of Mutual Economic Aid countries in 55.5%. The obtained results of fluorine and lead determinations were compared with those in the years 1981-1983 stressing the health hazards caused by consumption of vegetables and fruit from the gardens and fields situated in the vicinity of glass plant emission sources.     

Memory complaints in epilepsy: An accurate reflection of memory impairment or an indicator of poor adjustment? A Review of the literature

People with epilepsy frequently complain of poor memory. Although organic memory impairment is one possible sequela of neuro-epilepsy variables, these complaints are not consistently supported by performance on objective measures. The current review has two objectives: first, to establish whether inconsistent results are an artifact of methodology and second, to collate existing published literature to identify possible explanations for inaccurate memory self-report in epilepsy. Review of the literature highlights many methodological limitations making it difficult to evaluate findings. However, it is apparent that in people with epilepsy, subjective memory demonstrates a greater relationship with anxiety and depression than with objective memory. We examine the hypothesis that memory complaints in epilepsy are a reflection of difficulties adjusting to, or coping with, the condition. Research has yet to identify any critical variables or pathways through which these factors influence perceptions of memory function. This review proposes a role for illness representations in understanding the nature of memory complaints in epilepsy.     

Rapid onset of seizure suppression with pregabalin adjunctive treatment in patients with partial seizures

Purpose:  To determine the time at which pregabalin demonstrates seizure-suppressing activity when given as adjunctive treatment to patients with refractory partial seizures.
Methods:  Data from four similar 12-week, randomized, double-blind, placebo-controlled, parallel-group trials in patients with refractory partial seizures were pooled to provide an adequate sample to compare the proportion of patients free of seizures on each study day between pregabalin (combined 150–600 mg/day groups) and placebo (combined groups). A generalized estimating equation (GEE) statistical model was used to perform pairwise comparisons on each study day. In several pregabalin dosage groups the dosage was escalated during days 1–7, whereas in others pregabalin was initiated at a fixed dosage without escalation.
Results:  The proportion of patients free of seizures on any treatment day was greater in the combined pregabalin groups compared with baseline. Differences were not observed between the placebo group and baseline. A significantly greater proportion of patients were free of seizures in the combined pregabalin 150–600 mg/day and the pregabalin 600 mg/day fixed-dosage groups compared with the placebo groups from treatment day 2 onward (p < 0.05). From day 8 (coinciding with completion of the 1-week dosage-escalation period in two studies) onward, the proportion of patients free of seizures per day in the pregabalin groups remained relatively constant.
Discussion:  This exploratory analysis of a refractory population using a rigorous endpoint demonstrates that pregabalin rapidly reduced the frequency of partial seizures. At the dosing schemes most commonly used in placebo-controlled trials, significant seizure-suppressing activity was observed after only 2 days of treatment.     

Pheochromocytoma: challenges in diagnosis and nursing care

Pheochromocytomas are catecholamine-secreting tumors arising from chromaffin cells of the sympathoadrenal system, which includes the adrenal medulla and sympathetic ganglionic tissue. The effects of catecholamine excess cause potentially fatal symptomologies and end-organ damage if not diagnosed and treated. If diagnosed and removed surgically, most of these patients can be cures. Pheochromocytomas are rare and affect from two to eight per million people. There are 800 deaths in the United States annually as a result of complications. Of patients who have pheochromocytomas diagnosed at autopsy, 75% died suddenly from myocardial infarction or cerebral vascular catastrophe. Challenges in diagnosis, tumor location, and treatment are considerable.     

Age and weight effects on motor nerve conduction time measurements in an asymptomatic industrial population

Motor conduction delay has been demonstrated in patients with carpal tunnel syndrome (CTS), however, there is also evidence that conduction slowing can be effected by other factors, e.g., the aging process. Recent development of the digital electroneurometer, a hand-held, battery-powered device for measuring motor nerve conduction time, has made this test available for CTS screening in industry. A cross-sectional study of 77 industrial workers was performed to determine the relationship between median motor nerve conduction time at the carpal tunnel and select personal factors. Motor nerve conduction time increased with age, length of employment, and weight. However, these variables accounted for only 20.3% of the variance in conduction time. The study indicates users of this device should be aware of these effects, and that other sources of variation must be identified and controlled if motor nerve conduction tests are to be used for CTS screening in industry.     

Proteomic analysis of neuroblastoma subtypes in response to mitogen-activated protein kinase inhibition: profiling multiple targets of cancer kinase signaling

INTRODUCTION: Survival for high-risk neuroblastoma (NB) remains poor despite aggressive therapy. Novel therapies are vital for improving prognosis. We previously showed differential NB subtype sensitivity to p42/44 mitogen-activated protein kinase (ERK/MAPK) pathway inhibition. In this study, we investigated proteomic changes associated with resistance or sensitivity to MAPK kinase (MEK) inhibition in NB subtypes. MATERIALS AND METHODS: SH-SY5Y (N-type), BE(2)-C (I-type), and SK-N-AS (S-type) were treated with MEK inhibitor U0126 (10 microM) for 1 and 24 h. Proteins were extracted from untreated and treated cells and analyzed for differential expression by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Selected polypeptides were extracted from the gel and identified by liquid chromatography-linked tandem mass spectrometry (LC-MS/MS). RESULTS: We identified 15 proteins that were decreased by 2.5-fold between untreated and 1 h treated cells and subsequently up-regulated 5-fold after 24 h drug treatment. N-type NB (MEK-resistant) showed the least altered proteomic profile whereas the I-type (MEK-sensitive) and S-type NB (MEK-intermediate) generated significant protein changes. The majority of proteins identified were induced by stress. CONCLUSIONS: Protein differences exist between MEK inhibitor-treated NB subtypes. Identified polypeptides all have roles in mediating cellular stress. These data suggest that inhibition of the ERK/MAPK in NB subtypes leads to an intracellular stress response. The most resistant NB cell line to MEK inhibitor treatment generated the least protective protein profile, whereas the intermediate and most sensitive NB cells produced the most stress response. These findings suggest stress related protein expression may be targeted in assessing a response to ERK/MAPK therapeutics.