排序方式: 共有33条查询结果,搜索用时 31 毫秒
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Bray Emily E. Zheng Zihan Tolbert M. Katherine McCoy Brianah M. Kaeberlein Matt Kerr Kathleen F. 《Age (Dordrecht, Netherlands)》2022,44(3):1779-1790
GeroScience - A variety of diets have been studied for possible anti-aging effects. In particular, studies of intermittent fasting and time-restricted feeding in laboratory rodents have found... 相似文献
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Murakami CJ Burtner CR Kennedy BK Kaeberlein M 《The journals of gerontology. Series A, Biological sciences and medical sciences》2008,63(2):113-121
Chronological aging in yeast has been studied by maintaining cells in a quiescent-like stationary phase culture and monitoring cell survival over time. The composition of the growth medium can have a profound influence on chronological aging. For example, dietary restriction accomplished by lowering the glucose concentration of the medium significantly increases life span. Here we report a novel high-throughput method for measuring yeast chronological life span by monitoring outgrowth of aging cells using a Bioscreen C MBR machine. We show that this method provides survival data comparable to traditional methods, but with decreased variability. In addition to reducing the glucose concentration, we find that elevated amino acid levels or increased osmolarity of the growth medium is sufficient to increase chronological life span. We also report that life-span extension from dietary restriction does not require any of the five yeast sirtuins (Sir2, Hst1, Hst2, Hst3, or Hst4) either alone or in combination. 相似文献
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Pitt Jason N. Strait Nolan L. Vayndorf Elena M. Blue Benjamin W. Tran Christina H. Davis Brendon E. M. Huang Karen Johnson Brock J. Lim Keong Mu Liu Sophie Nikjoo Arash Vaid Anuj Wu Judy Z. Kaeberlein Matt 《Age (Dordrecht, Netherlands)》2019,41(6):961-973
GeroScience - Caenorhabditis elegans is a popular organism for aging research owing to its highly conserved molecular pathways, short lifespan, small size, and extensive genetic and reverse genetic... 相似文献
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Payel Sen Weiwei Dang Greg Donahue Junbiao Dai Jean Dorsey Xiaohua Cao Wei Liu Kajia Cao Rocco Perry Jun Yeop Lee Brian M. Wasko Daniel T. Carr Chong He Brett Robison John Wagner Brian D. Gregory Matt Kaeberlein Brian K. Kennedy Jef D. Boeke Shelley L. Berger 《Genes & development》2015,29(13):1362-1376
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Kaeberlein M Kirkland KT Fields S Kennedy BK 《Mechanisms of ageing and development》2005,126(4):491-504
Here we describe the replicative life spans of more than 50 congenic Saccharomyces cerevisiae strains, each carrying a mutation previously implicated in yeast aging. This analysis provides a direct comparison, in a single, long-lived strain background, of a majority of reported yeast aging genes. Of the eleven deletion mutations previously reported to increase yeast life span, we find that deletion of FOB1, deletion of SCH9, and deletion of GPA2, GPR1, or HXK2 (three genetic models of calorie restriction) significantly enhanced longevity. In addition, over-expression of SIR2 or growth on low glucose increased life span. These results define a limited number of genes likely to regulate replicative life span in a strain-independent manner, and create a basis for future epistasis analysis to determine genetic pathways of aging. 相似文献
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Siyoung Cho Miook Cho Juewon Kim Matt Kaeberlein Sang Jun Lee Yousin Suh 《Oncotarget》2015,6(1):43-55
Hypoxia-inducible factor 1 (HIF-1) is a master regulator of hypoxic response and has been a prime therapeutic target for ischemia/reperfusion (I/R)-derived myocardial dysfunction and tissue damage. There is also increasing evidence that HIF-1 plays a central role in regulating aging, both through interactions with key longevity factors including Sirtuins and mTOR, as well as by directly promoting longevity in Caenorhabditis elegans. We investigated a novel function and the underlying mechanism of syringaresinol, a lignan compound, in modulation of HIF-1 and protection against cellular damage and death in a cardiomyocyte model of I/R injury. Syringaresinol caused destabilization of HIF-1α following H/R and then protected against hypoxia/reoxygenation (H/R)-induced cellular damage, apoptosis, and mitochondrial dysfunction in a dose-dependent manner. Knock-down of FOXO3 by specific siRNAs completely abolished the ability of syringaresinol to inhibit HIF-1 stabilization and apoptosis caused by H/R. Syringaresinol stimulated the nuclear localization and activity of FOXO3 leading to increased expression of antioxidant genes and decreased levels of reactive oxygen species (ROS) following H/R. Our results provide a new mechanistic insight into a functional role of syringaresinol against H/R-induced cardiomyocyte injury and death. The degradation of HIF-1α through activation of FOXO3 is a potential therapeutic strategy for ischemia-related diseases. 相似文献